레몬그라스 에탄올 추출물의 3T3-L1 지방세포 분화 억제효과
Inhibitory Effect of Cymbopogon Citratus Ethanol Extracts on Adipogenesis in 3T3-L1 Preadipocytes
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초록
Cymbopogon citratus, commonly know as lemongrass, prossesses strong antioxidant, anti-tumor and anti-inflammatory properties. Howerver, its anti‑obesity activity remains to be elucidated. This study investigated the effect of ethanol extract of Cymbopogon citratus on adipogenesis, and its underlying mechanism, in 3T3-L1 preadipocytes. The results demonstrated that ethanol extracts of Cymbopogon citratus effectively suppressed intercellular lipid accumulation at non-toxic concentrations, and was associated with the down-regulation of adipocyte-specific transcription factors, including C/EBPα and PPARγ, and phosphorylation of AMPKα. Furthermore, ethanol extracts of Cymbopogon citratus increased p21 and p21 expression, while the expression of CDK2, cyclin A and cyclin B1 was reduced. As a result, ethanol extracts of Cymbopogon citratus seems to induce G0/G1 cell cycle arrest of 3T3-L1 cells. On the other hand, ERK and Akt signaling pathways were not involved in anti-adipogenesis by ethanol extracts of Cymbopogon citratus. Taken together, theses results suggest that ethanol extracts of Cymbopogon citratus inhibits adipocyte differentiation in 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.
Abstract
Cymbopogon citratus, commonly know as lemongrass, prossesses strong antioxidant, anti-tumor and anti-inflammatory properties. Howerver, its anti‑obesity activity remains to be elucidated. This study investigated the effect of ethanol extract of Cymbopogon citratus on adipogenesis, and its underlying mechanism, in 3T3-L1 preadipocytes. The results demonstrated that ethanol extracts of Cymbopogon citratus effectively suppressed intercellular lipid accumulation at non-toxic concentrations, and was associated with the down-regulation of adipocyte-specific transcription factors, including C/EBPα and PPARγ, and phosphorylation of AMPKα. Furthermore, ethanol extracts of Cymbopogon citratus increased p21 and p21 expression, while the expression of CDK2, cyclin A and cyclin B1 was reduced. As a result, ethanol extracts of Cymbopogon citratus seems to induce G0/G1 cell cycle arrest of 3T3-L1 cells. On the other hand, ERK and Akt signaling pathways were not involved in anti-adipogenesis by ethanol extracts of Cymbopogon citratus. Taken together, theses results suggest that ethanol extracts of Cymbopogon citratus inhibits adipocyte differentiation in 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.
참고문헌 (39)
- Peng Y, "MicroRNAs : Emerging roles in adipogenesis and obesity", Cell Signal, 26(9), pp.1888~1896 (2014)
- Jo J, "Hypertrophy and", or hyperplasia: dynamics of adipose tissue growth, 2009, pp.5(3), e1000324~
- 오정숙, "한국 중년 여성의 비만과 관련된 우울군과 정상군 간의 식습관 및 생활 행태 비교", 아시안뷰티화장품학술지, 11(2), pp.231~238 (2013), 한국피부과학연구원
- 정미영, "체질량지수와 주관적 체형 인식에 따른 비만관련 생활행태 비교", 아시안뷰티화장품학술지, 11(6), pp.1109~1115 (2013), 한국피부과학연구원
- Gwon SY, "Shikonin suppresses ERK 1", 2 phosphorylation during the early stages of adipocyte differentiation in 3T3-L1 cells, 2013, pp.13, 207~
- Cornelius P, "Regulation of adipocyte development", Annu Rev Nutr, 14, pp.99~129 (1994)
- Prusty D, "Activation of MEK", ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma(PPARgamma)and C, J Biol Chem, pp.2002, 277(48)[DOI]
- Xu J, "Protein kinase B", AKT 1 plays a pivotal role in insulin-like growth factor-1 receptor signaling induced 3T3-L1 adipocyte differentiation, 2004, pp.279(34), 35914~35922
- Rosen ED, "PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro", Mol Cell, 4(4), pp.611~617 (1999)
- Wu Z, "Cross-regulation of C", EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity, 1999, pp.3(2), 151~158
- 손명수, "3T3-L1 세포에서 Adipogenesis 억제를 통한 Kaki-tannin의 항비만 활성", 아시안뷰티화장품학술지, 12(4), pp.539~546 (2014), 한국피부과학연구원
- Gupta R, "Lipase assays for conventional and molecular screening: an overview", Biotechnol Appl Biochem, 37(Pt 1), pp.63~71 (2003)
- 김소혜, "비만성인의 영양소 섭취량 및 식사 다양성 평가", 대한지역사회영양학회지, 12(5), pp.583~591 (2007), 대한지역사회영양학회
- 이미란, "레몬그라스 식초의 초산발효 특성과 항산화 활성", Food Science and Preservation, 24(5), pp.680~687 (2017), 한국식품저장유통학회
- Bhattacharya AK, "Effect of Prolonged Storage on the Quality of Lemongrass (Cymbopogon flexuosus (Nees ex Steud.) Wats.) Essential Oil", J Essent Oil Bear Pl, 1(2-3), pp.104~109 (1998)
- Sforcin JM, "Lemongrass effects on IL-1beta and IL-6 production by macrophages", Nat Prod Res, 23(12), pp.1151~1159 (2009)
- Francisco V, "Chemical characterization and anti-inflammatory activity of luteolin glycosides isolated lemongrass", J Funct Foods, 10, pp.436~443 (2014)
- Silva CB, "Antifungal activity of the lemongrass oil and citral against Candida spp", Braz J Infect Dis, 12(1), pp.63~66 (2008)
- Sri Devi S, "Citral, a Monoterpene Inhibits Adipogenesis Through Modulation of Adipogenic Transcription Factors in 3T3-L1 Cells", Indian J Clin Biochem, 33(4), pp.414~421 (2018)
- Modak T, "Effects of citral, a naturally occurring antiadipogenic molecule, on an energy-intense diet model of obesity", Indian J Pharmacol, 43(3), pp.300~305 (2011)
- Cheel J, "Free radical scavengers and antioxidants from Lemongrass (Cymbopogon citratus (DC.) Stapf.)", J Agric Food Chem, 53(7), pp.2511~2517 (2005)
- Lee MS, "Ethanol extract of Pinus koraiensis leaves containing lambertianic acid exerts anti-obesity and hypolipidemic effects by activating adenosine monophosphate-activated protein kinase(AMPK)", BMC Complement Altern Med, 16, pp.51~ (2016)
- Tang QQ, "Mitotic clonal expansion : a synchronous process required for adipogenesis", Proc Natl Acad Sci USA, 100(1), pp.44~49 (2003)
- 손명수, "3T3-L1 세포에서 울금(Curcuma longa L.) 추출물의 항비만 및 항산화 활성", 아시안뷰티화장품학술지, 12(2), pp.169~175 (2014), 한국피부과학연구원
- Farmer SR, "Transcriptional control of adipocyte formation", Cell Metab, 4(4), pp.263~263 (2006)
- Tontonoz P, "Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor", Cell, 79(7), pp.1147~1156 (1994)
- Carling D, "A common bicyclic protein kinase cascade inactivates the regulatory enzymes of fatty acid and cholesterol biosynthesis", FEBS Lett, 223(2), pp.217~222 (1987)
- Hardie DG, "Regulation of lipid metabolism by the AMP-activated protein kinase", Biochem Soc Trans, 25(4), pp.1229~1231 (1997)
- Hardie DG, "Regulation of fatty acid synthesis and oxidation by the AMP-activated protein kinase", Biochem Soc Trans, 30(Pt 6), pp.1064~1070 (2002)
- Chen S, "Resveratrol inhibits cell differentiation in 3T3-L1 adipocytes via activation of AMPK", Can J Physiol Pharmacol, 89(11), pp.793~799 (2011)
- Lee YK, "Curcumin exerts antidifferentiation effect through AMPKalpha-PPAR-gamma in 3T3-L1 adipocytes and antiproliferatory effect through AMPKalpha-COX-2 in cancer cells", J Agric Food Chem, 57(1), pp.305~310 (2009)
- Rosen ED, "PPARgamma : a nuclear regulator of metabolism, differentiation, and cell growth", J Biol Chem, 276(41), pp.37731~37734 (2001)
- Rosen ED, "Adipocyte differentiation from the inside out", Nat Rev Mol Cell Biol, 7(12), pp.885~896 (2006)
- Smith PJ, "Insulin‑like growth factor‑I is an essential regulator of the differentiation of 3T3‑L1 adipocytes", J Biol Chem, 263(19), pp.9402~9408 (1988)
- Bost F, "The role of MAPKs in adipocyte differentiation and obesity", Biochimie, 87(1), pp.51~56 (2005)
- Magun R, "Expression of a constitutively activated form of protein kinase B(c‑Akt)in 3T3‑L1 preadipose cells causes spontaneous differentiation", Endocrinology, 137(8), pp.3590~3593 (1996)
- Peng XD, "Dwarfism, impaired skin development, skeletal muscle atrophy, delayed bone development and impeded adipogenesis in mice lacking Akt1 and Akt2", Genes Dev, 17(11), pp.1352~1365 (2003)
- Kim SH, "Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells", Biochem Biophys Res Commun, 372(1), pp.108~113 (2008)
- Jang YJ, "Theobromine inhibits differentiation of 3T3-L1 cells during the early stage of adipogenesis via AMPK and MAPK signaling pathways", Food Funct, 6(7), pp.2365~2374 (2015)
