LPS로 유도된 RAW 264.7 cell의 염증반응에서 淸肺瀉肝湯의 항염증 효과
Effect of Cheongpyesagan-tang on LPS Induced Inflammation in RAW 264.7 Cells
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
Cheongpyesagan-tang (CP) is one of the traditional medicinal prescription to treat Taeumin (太陰人)`s disease. It has been commonly used for the treatment of stroke, arthritis, diabetes and obesity. In this study, we investigated an anti-inflammatory potential of CP water extract. We examined the effects of CP on the lipopolysarccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2). We also examined the levels of protein or mRNA of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and proinflammatory cytokines in RAW 264.7 cells. CP inhibited NO and PGE2 production in a dose dependent manner and decreased the protein and mRNA expression of iNOS and COX-2. Also, CP decreased the mRNA expression of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α). These results suggest that CP has potential as anti-inflammatory therapeutic medicine.
Abstract
Cheongpyesagan-tang (CP) is one of the traditional medicinal prescription to treat Taeumin (太陰人)`s disease. It has been commonly used for the treatment of stroke, arthritis, diabetes and obesity. In this study, we investigated an anti-inflammatory potential of CP water extract. We examined the effects of CP on the lipopolysarccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2). We also examined the levels of protein or mRNA of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and proinflammatory cytokines in RAW 264.7 cells. CP inhibited NO and PGE2 production in a dose dependent manner and decreased the protein and mRNA expression of iNOS and COX-2. Also, CP decreased the mRNA expression of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α). These results suggest that CP has potential as anti-inflammatory therapeutic medicine.
참고문헌 (35)
- Kindt TJ, "Innate immunity : Tenney S. Kuby Immunology", pp.52~73 (2007), Freeman press
- Jou IL, "Chronic Inflammatory Disease Research Center", pp.122~ (2012), Ajou University
- 윤현정, "마우스 대식세포인 RAW 264.7 세포에서 茵陳蒿湯의 항염증 효과", 대한본초학회지, 23(2), pp.169~178 (2008), 대한본초학회
- Textbook Compilation Committee of Pathology in Korean Medicine, "Pathology in Korean Medicine", pp.54~57 (2017), Hanuimunhwasa
- National College of Korean Medicine Collaborative Textbook Compilation Committee, "Herbology", pp.127~131 (2000), Younglim Publishing Inc
- Lee JM, "Dongeuisusebowon", pp.7~13 (1986), Henglim Publishing Inc
- 황지호, "熱多寒少湯과 淸肺瀉肝湯의 활용에 대한 임상적 연구", 한국한의학연구원논문집, 14(2), pp.101~106 (2008), 한국한의학연구원
- 최동준, "급성기 중풍 환자에 대한 청폐사간탕의 임상적 효능", 대한한의학회지, 23(4), pp.659~664 (2002), 대한한의학회
- 정춘근, "淸肺瀉肝湯이 糖尿誘發 흰쥐의 腦虛血損傷에 미치는 影響", 대한한의학회지, 26(2), pp.217~230 (2005), 대한한의학회
- 김정열, "淸肺瀉肝湯이 CCl4로 誘發된 휜쥐의 肝損傷에 미치는 影響", 사상체질의학회지, 17(1), pp.130~141 (2005), 사상체질의학회
- 안훈모, "淸肺瀉肝湯과 YKK012의 항암제 CPT-11과 병용투여 시 대장암 성장억제에 미치는 효과", 대한본초학회지, 30(1), pp.33~42 (2015), 대한본초학회
- 박영주, "청폐사간탕(淸肺瀉肝湯)의 혈소판 응집억제 작용에 대한 in vitro 연구", 대한한방내과학회지, 31(4), pp.714~721 (2010), 대한한방내과학회
- Hong SG, "Protective effects of Chungpesagan-tang against ischemia", reperfusion induced cell injury, 2000, pp.54(1), 111~117
- Kim DL, "An experimental study of the effect by Chongshimyonjatang and Chongpyesagantang on immune response and anti-allergic reaction to rats and mice", J KyungHee Orient Med Coll, 14, pp.131~160 (1991)
- Kim JH, "The Effect of Chungpyesagantang on Lipopolysaccharide induced Arthritis in Mice", J Sasang Const Med, 14(3), pp.114~131 (2002)
- Koo JS, "Effects of Chungpyesagantang on the diabetic rats induced by streptozotocin", J Korean Orient Pediatr, 11(1), pp.227~248 (1997)
- Song MY, "The clinical study about the effect of Chugpaesagan-tang and Taeumjoui-tang on Taeumin obesity after fasting therapy", J Orient Rehab Med, 8(1), pp.34~56 (1998)
- 김은영, "태음인 청폐사간탕이 lipase 활성저해 및 db", db mouse의 체중감량, 혈장지질, UCP 1, 2 발현에 미치는 영향, 2007, pp.19(1), 171~185[DOI]
- Bae JH, "Effect of Cheongpesagan-tang on the changes of the weight, plasma and UCP mRNA expression in obese SD rats induced high fat feed", J Orient Rehabil Med, 12(1), pp.133~156 (2002)
- Huh SY, "Experimental study on the effect of Chungpesagantang in obese rats", J Orient Rehabil Med, 8(2), pp.106~118 (1998)
- 김범회, "LPS로 유도된 RAW264.7 염증모델에서 MAPK 조절에 의한 羊乳의 항염증효과", 동의생리병리학회지, 24(1), pp.80~84 (2010), 한의병리학회
- Dawson TM, "A novel neuronal messenger molecule in brain : The free radical, nitric oxide", Ann Neurol, 32, pp.297~311 (1992)
- Wang MJ, "Silymarin protects dopaminergic neurons against lipopolysaccharide-induced neurotoxicity by inhibiting microglia activation", Eur J Neurosci, 16(11), pp.2103~2112 (2002)
- Hanisch UK, "Microglia as a source and target of cytokines", Glia, 40(2), pp.140~155 (2002)
- Chen W, "Role of p38 MAP kinases and ERK in mediating ultraviolet-B induced cyclooxygenase-2 gene expression in human keratinocytes", Oncogene, 20(29), pp.3921~3926 (2001)
- Marina L, "Regulation of Cyclooxygenase 2 mRNA Stability by the Mitogen-Activated Protein Kinase p38 Signaling Cascade", Mol Cell Biol, 20(12), pp.4265~4274 (2000)
- Mitchell JA, "Cyclooxygenase-2 : regulation and relevance in inflammation", Biochemical Pharmacology, 50(10), pp.1535~1542 (1995)
- Crofford LJ, "Basic biology and clinical application of specific cyclooxygenase-2 inhibitors", Arthritis Rheum, 43(1), pp.4~13 (2000)
- Raabe T, "Relative contribution of transcription and translation to the induction of tumor necrosis factor-alpha by lipopolysaccharide", J Biol Chem, 273(2), pp.974~980 (1998)
- 이범준, "한국 홍삼과 중국 홍삼의 경구 투여가 흰쥐의 림프구 증식과 Cytokine에 미치는 영향에 대한 비교 연구", Journal of Ginseng Research, 32(3), pp.250~256 (2008), 고려인삼학회
- Lee ES, "Inhibition of nitric oxide and tumor necrosis factor-α(TNF-α)production by propenone compound through blockade of nuclear factor(NF)-κB activation in cultured murine macrophage", Biol Pharm Bull, 27(5), pp.617~620 (2004)
- Lazarov S, "The role of bacterial endotoxins, receptors and cytokines in the pathogenisis of septic(endotoxin)shock", Vutr Boles, 32(4), pp.33~40 (2000)
- Berek JS, "Serum interleukin-6 levels correlate with disease status in patients with epithelial ovarian cancer", Am J Obstet Gynecol, 164(4), pp.1038~1042 (1991)
- Dupont N, "Autophagy-based unconventional secretory pathway for extracellular delivery of IL-1β", EMBO J, 30, pp.4701~4711 (2011)
- Narayanan P, "Tumor Necrosis Factor-α Signaling in Macrophages", Crit Rev Eukaryot Gene Expr, 20(2), pp.87~103 (2010)
