갈근탕 혼합단미엑스제 및 전탕액의 성분 및 생리활성 비교 연구
Comparison of Ingredient and Efficacy of Galgeun-tang (Gegen-tang) Mix Extract Powder and Decoction
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Galgeun-tang (GGT) is a traditional herbal medicine generally used to treat cold, fever, headache, migraine and convulsion. To investigate the difference of ingredient and efficacy of GGT according to production method, mix extract powder and decoction, the quantities of index components, antioxidant and anti-inflammatory effects of two kinds of GGT formulation were compared. The contents of ten index components were simultaneously analyzed with HPLC. A HPLC method was developed and validated for the simultaneous determination. The GGT mix extract powder contained higher puerarin and daidzin content than the decoction, while the GGT decoction contained higher cinnamic acid and glycyrrhizic acid content than the mix extract powder. But ingredient of GGT mix extract powder and decoction was statistically very similar (r=0.8335). GGT decoction showed higher antioxidant effect than GGT mix extract powder, but the difference was low. The patterns of productions for NO, iNOS, COX-2, TNF-α, IL-1β and IL-6 were decreased along the increasing dose of GGT formulation in LPS-induced RAW 264.7 macrophages. The inhibitory effects on NO, iNOS, COX-2, IL-1β and IL-6 in Raw 264.7 cell were very similar to each other. The results obtained in this study could be used as fundamental data for verifying the equivalence and effectiveness of the herbal medicines.
Abstract
Galgeun-tang (GGT) is a traditional herbal medicine generally used to treat cold, fever, headache, migraine and convulsion. To investigate the difference of ingredient and efficacy of GGT according to production method, mix extract powder and decoction, the quantities of index components, antioxidant and anti-inflammatory effects of two kinds of GGT formulation were compared. The contents of ten index components were simultaneously analyzed with HPLC. A HPLC method was developed and validated for the simultaneous determination. The GGT mix extract powder contained higher puerarin and daidzin content than the decoction, while the GGT decoction contained higher cinnamic acid and glycyrrhizic acid content than the mix extract powder. But ingredient of GGT mix extract powder and decoction was statistically very similar (r=0.8335). GGT decoction showed higher antioxidant effect than GGT mix extract powder, but the difference was low. The patterns of productions for NO, iNOS, COX-2, TNF-α, IL-1β and IL-6 were decreased along the increasing dose of GGT formulation in LPS-induced RAW 264.7 macrophages. The inhibitory effects on NO, iNOS, COX-2, IL-1β and IL-6 in Raw 264.7 cell were very similar to each other. The results obtained in this study could be used as fundamental data for verifying the equivalence and effectiveness of the herbal medicines.
참고문헌 (26)
- 박해모, "한약재 복용으로 인한 금속 섭취량 추정 및 위해성 평가 연구", 한국환경보건학회지, 32(2), pp.186~191 (2006), 한국환경보건학회
- 김은정, "시판 황련해독탕 Ex제제의 성분함량 모니터링 연구", 대한한의학 방제학회지, 16(1), pp.95~107 (2008), 대한한의학방제학회
- 김정훈, "전탕 조건에 따른 쌍화탕 전탕액 비교 연구", 대한한의학 방제학회지, 18(2), pp.125~134 (2010), 대한한의학방제학회
- Bi Z, "Study on the variation of the main constituents in Gardeniae Fructus after compatibility with Scutellariae Radix", Pharm Clini Res, 19, pp.29~31 (2011)
- Kim NM, "Quality change of cinnamon extract prepared with various drying methods", Korean J Food & Nutr, 13, pp.152~157 (2000)
- Kim NM, "Effects of solvents and some extraction conditions on antioxidant activity in cinnamon extracts", Korean J Food Sci Technol, 25(3), pp.204~209 (1993)
- Bansky D, "Chinese Herbal Medicine Formulas and Strategies", pp.7~8 (1990), Eastland Press
- Korea Food and Drug Administration, "The Korean Herbal Pharmacopoeia" (2016)
- Yang TK, "An Experimental study on the anti-allergic effects, anti-inflammatory action, anti-pyretic action and analgesic action of Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang", J Orient Med Surgery, Ophthalmology & Otolaryngology, 15(1), pp.76~95 (2002)
- Korea Food and Drug Administration, "Regulation on Approval and Notification of Herbal (crude) Medicinal Preparations", Etc. KFDA Notification No. 2016-112, ~ (2016)
- Korea Food and Drug Administration, "Analytical method guideline about validation of drug and etc", ~ (2015)
- Blois MS, "Antioxidant determinations by the use a stable free radical", Nature, 26, pp.1199~1200 (1958)
- Zhishen J, "The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals", Food Chem, 64(4), pp.555~559 (1999)
- Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", Am J Enol Vitic, 16(3), pp.144~158 (1965)
- 마진열, "폴리페놀 및 DPPH 라디칼 소거활성 관점에서의 복합갈근탕 및 단미혼합갈근탕의 동등성", 약 학 회 지, 55(1), pp.45~48 (2011), 대한약학회
- Yun YG., "East Medical Traditional Medical Formulae and prescription Commentary", pp.145~ (1998), Euiseongdang
- 최성모, "Theoretical Chemical Reaction for Herb Medicine", Journal of Pharmacopuncture, 5(2), pp.116~119 (2002), 대한약침학회[DOI]
- 이상명, "인삼 탕액 제조시 구연산과 오미자에 의한 진세노사이드 조성 변화", 생약학회지, 45(2), pp.121~126 (2014), 한국생약학회
- 이준경, "표준한방처방의 EBM 구축을 위한 葛根湯 문헌 분석 연구", 대한한의학 방제학회지, 19(1), pp.103~111 (2011), 대한한의학방제학회
- 하혜경, "Galgeun-tang, an Herbal Formula, Ameliorates Atopic Dermatitis Responses in Dust Mite Extract-treated NC", Nga Mice, 2013, pp.34(4), 1~11[DOI]
- 김예지, "용매별 갈근탕 추출물의 성분 분석 및 효능 비교", 동의생리병리학회지, 26(6), pp.908~914 (2012), 한의병리학회
- Shin NR, "Galgeun-tang Attenuates Cigarette Smoke and Lipopolysaccharide Induced Pulmonary Inflammation via Iκ-Bα", NF-κB Signaling, 2018, pp.23(10), 2489~2499
- Wynn TA, "Macrophage biology in development homeostasis and disease", Nature, 496(7446), pp.445~455 (2013)
- 김온순, "삼출건비탕 전탕액과 시판 한약제제의 비교 연구", 대한한의학 방제학회지, 20(2), pp.1~11 (2012), 대한한의학방제학회
- Kang KH, "The effect of Sagan-tang applied to conjunctivitis on hepatic damage in rats", Kyunghee Univ Oriental Med J, 8(1), pp.185~194 (1985)
- Lee SD, "The effects of Samhwangsasimtang on the blood pressure and serum in S.H.R", Kyunghee Univ Oriental Med J, 7(1), pp.181~191 (1984)