소염약침의 LPS로 유도된 RAW 264.7세포의 염증성 사이토카인 발현에 미치는 영향
Inhibitory Effects of Soyeum Pharmacopuncture(SPP) on LPS-induced Inflammation Related Cytokine Expressions of RAW 264.7 cells
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Abstract
Aim : This study was done to investigate whether SPP has inhibitory effects on the activation of RAW 264.7 cells. Method : In tumor necrosis factor-a (TNF-α)/ interleukin-1b (IL-1β) and IL-6, the mRNA expression of molecular indicators related to inflammatory changes of the Reumatoid Arthritis (RA) were examined using quantitative real-time PCR. Results : The treatment of SPP significantly suppressed the expression of proinflammatory cytokines and chemokines such as TNF-α, IL-1β, IL-6 compared with the control. The expression of NOS-II was considerably reduced, which was accompanied by a reduction in the production of nitric oxide (NO). It also reduced the expression of TNF-α in serum of Balb/c mice compared with control group. Conclusion : SPP may be an effective herbal material for suppressing the inflammation related cytokines of RAW 264.7 cells.
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