상황버섯 균사체를 이용한 전통주의 추출물이 HepG2 세포의 염증관련 단백질 발현에 미치는 영향
Effects of Traditional Wine by using Mycelium of Phellinus linteus on the Expression of Inflammation-Related Proteins in HepG2 Cells
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Abstract
It was examined that the effect of fermented traditional wine made by using mycelium of Phellinus linteus on the expression of inflammation-related proteins in HepG2 cells. HepG2 cells were incubated with or without extract of traditional wine (ETMP), then analyzed by microscopic observation, reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The results of RT-PCR and Western blot analyses showed that the level of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α was induced by LPS, but the treatment of ETMP inhibited the expression of these proteins and its mRNAs. Besides, the results of Western blot analyses showed that the expression of nuclear factor-κBp65 and inhibitory-κBα were also slightly affected by ETMP treatment. These results suggest that ETMP alleviate the expression of inflammation-related protein expressions and thus may be used as a functional alcoholic beverage.
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