Moutan Root Bark가 항산화활성과 LDL 산화 억제 및 항혈소판 응집에 미치는 영향
Screening of Antioxidative, Anti-thrombotic and Anti-atherosclerotic Effects of Moutan Root Bark Extracts
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There is currently increased interest in the identification of antioxidant compounds that are pharmacologically potent and have low or no side effects. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. Moutan Root Bark (MRB) has been frequently used as analgesic, antispasmodic, anti-inflammatory and remedies for female diseases. In this study, the antioxidant activity of extract from MRB was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The MRB extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the MRB extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.
Abstract
There is currently increased interest in the identification of antioxidant compounds that are pharmacologically potent and have low or no side effects. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. Moutan Root Bark (MRB) has been frequently used as analgesic, antispasmodic, anti-inflammatory and remedies for female diseases. In this study, the antioxidant activity of extract from MRB was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The MRB extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the MRB extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.
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