Lonicera Flower의 항산화활성과 LDL 산화 억제효과 및 혈전용해능에 관한 연구
Screening of Anti-atherosclerotic Effect of Lonicera Flower by Antioxidative and Anti-thrombotic Mechanism
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The flowers and buds of Lonicera Flower (LF), are used in Korean herbal medicine for latent-heat-clearing, antipyretic, detoxicant and anti-inflammatory ailments. This plant is used worldwide for the treatment of many types of inflammatory disease including respiratory infections, diabetes mellitus, rheumatoid arthritis and play an important role in immune reaction. These pharmaceutical effects of LF looks like to be related to its antioxidant capacity and phytochemicals containing in LF. In this study, the antioxidant activity of extract from LF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The LF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the LF extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.
Abstract
The flowers and buds of Lonicera Flower (LF), are used in Korean herbal medicine for latent-heat-clearing, antipyretic, detoxicant and anti-inflammatory ailments. This plant is used worldwide for the treatment of many types of inflammatory disease including respiratory infections, diabetes mellitus, rheumatoid arthritis and play an important role in immune reaction. These pharmaceutical effects of LF looks like to be related to its antioxidant capacity and phytochemicals containing in LF. In this study, the antioxidant activity of extract from LF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The LF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the LF extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.
참고문헌 (43)
- 전국한의과대학본초학교수, "본초학", pp.198~198 (2000), 영림사
- 지형준, "대한약전외 한약(생약)규격집 주해서", pp.87~88 (1988), 한국메디칼인덱스사
- 한방약리학 교재편찬위원회, "한방약리학", pp.133~134 (2006), 신일상사
- 김철호, "한약약리학", pp.150~151 (2001), 집문당
- Lee, J.N., "Effects of aqueous extract from Lonicera japonica flower on trimellitic anhydride-induced contact hypersensitivity in BALB", c Mice, 2008, pp.23(2), 51~58
- Yun, Y.G., "Inhibitory effect of aqueous extract from Lonicera japonica flower on LPS-induced inflammatory mediators in RAW 264.7 macrophages", Kor. J. Herbology, 22(3), pp.117~125 (2007)
- Xu, Y., "Trifunctional inhibition of COX-2 by extracts of Lonicera japonica: Direct inhibition, transcriptional and post-transcriptional down regulation", Journal of Ethnopharmacology, 111(3), pp.667~670 (2007)
- Park, H.S., "The effects of anti-cancer response of Lonicerae Flos herbal-acupuncture", The Journal of Korean Acupuncture & Moxibustion Society, 22(5), pp.91~97 (2005)
- 배지현, "식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과", 한국식품과학회지, 37(4), pp.642~647 (2005), 한국식품과학회
- Giles, G.I., "Hypothesis: the role of reactive sulfur species in oxidative stress", Free Radical Biology & Medicine, 31, pp.1279~1283 (2001)
- Espey, M.G., "A chemical perspective on the interplay between NO, reactive oxygen species, and reactive nitrogen oxide species", Ann. N. Y Acad. Sci, 962, pp.195~206 (2002)
- Fubini, B., "Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis", Free Radical Biology & Medicine, 34, pp.1507~1516 (2003)
- "2006년 사망원인 통계 결과", 통계청[URL]
- Elisaf, M., "The treatment of coronary heart disease: an update. Part 1: An overview of the risk factors for cardiovascular disease", Curr. Med. Res. Opin, 17(1), pp.18~26 (2001)
- Raya, A.A., "Inflammation: A pivotal link between autoimmune diseases and atherosclerosis", Autoimmunity Reviews, 5, pp.331~337 (2006)
- Verstraeta, M., "Thrombolytic agents in development", Drugs, 50, pp.29~42 (1995)
- Nakajima, N., "Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus", Biosci. Biotechnol. Biochem, 57, pp.1726~1730 (1993)
- Roberta, R.E., "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology & Medicine, 26, pp.1231~1237 (1999)
- Miller, N.J., "A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates", Clin. Sci, 84, pp.407~412 (1993)
- Blois, M.S., "Antioxidant determination by the use of stable free radical", Nature, 26, pp.1199~1200 (1958)
- Gotoh, N., "Rates of interactions of superoxide with vitamin E, vitamin C and related compounds as measured by chemiluminescence", Biochem. Biophys. Acta, 1115, pp.201~207 (1992)
- Halliwell, B., "Role of free radicals and catalytic metalions in human disease: an overview", Method Enzymol, 186, pp.1~85 (1990)
- Nagata, N., "Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reactionof 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide", J. Biochem. Tokyo, 125, pp.658~661 (1999)
- Crow, J.P., "Dichlorodihydrofluorescein and dihydro- rhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species", Nitric Oxide, 1, pp.145~157 (1997)
- Yoon, M.A., "Antioxidant effect of quinoline alkaloid and 2,4-di-tert-butylphenol isolated from Scolopendra subspinipes", Biol. Pharm. Bull, 29, pp.735~739 (2006)
- Yagi, K.A., "Simple fluometric assay for lipoperoxide in blood plasma", Biochem. Med, 15, pp.212~216 (1976)
- Mullertz, S, "The fibrin method for estimating of fibrinolytic activity", Arch. Biochem. Biophys, 40, pp.346~351 (1952)
- Fogliano, V., "Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines", J. Agric. Food Chem, 47, pp.1035~1040 (1999)
- Nicoletta, P., "Total antioxidant capacity of plant foods, beverages and oils consumed in italy assessed by three different in vitro assays", J. Nutr, 133, pp.2812~2819 (2003)
- 남석현, "한약재 열수추출물의 항산화효과 검정", 한국농화학회지, 43, pp.141~147 (2000)
- Sanchez, C.S., "Different radical scavenging tests in virgin olive oil and their relation to the total phenol content", Anal. Chim. Acta, 593, pp.103~107 (2007)
- Chung, S.K., "Hydroxyl radical scavenging effects of spices and scavengers from brown mustard", Biosci. Biotech. Biochem, 61, pp.118~123 (1997)
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
- 이옥환, "올리브 잎 분획물의 항균활성 및 아질산염 소거능", 한국식품조리과학회지, 20(2), pp.204~210 (2004), 한국식품조리과학회
- 박찬성, "한약재 추출물의 항산화작용 및 아질산염 소거작용", 한국식품저장유통학회지, 12(6), pp.631~636 (2005), 한국식품저장유통학회
- Channon, K.M., "S.E. Nitric oxide synthase in atherosclerosis and vascular injury: insights from experimental gene therapy", Arterioscler. Thromb. Vasc. Biol, 20, pp.1873~1881 (2000)
- Virag, L., "Peroxynitrite induced cytotoxicity: metabolism and opportunities for intervention", Toxocol. Lett, pp.113~141 (2003)
- 서영완, "염생식물로부터 Peroxynitrite와 DPPH 라디칼 소거 활성 검색", KSBB Journal, 19(1), pp.57~61 (2004), 한국생물공학회
- Raya, A.A., "Inflammation: A pivotal link between autoimmune diseases and atherosclerosis", Autoimmunity Reviews, 5, pp.331~337 (2006)
- Berliner, J.A., "The role of oxidized lipoproteins in atherogenesis", Free Radic. Biol. Med, 20, pp.707~727 (1996)
- Yokoyama, M., "Oxidant stress and atherosclerosis", Current Opinion in Pharmacology, 4, pp.110~115 (2004)
- Zhenbao, J., "Antioxidant properties of extracts from juemingzi (Cassia tora L.) evaluated in vitro", LWT - Food Science and Technology, 40, pp.1072~1077 (2007)
- Packham, M.A., "Role of platelets in thrombosis and hemostasis", Can. J. of Physiol. Pharmacol, 72, pp.278~284 (1994)
