택사 열수 및 에탄올 추출물의 항산화활성과 human LDL 산화억제 및 ACE 저해효과에 미치는 영향
Screening of Antioxidative, Anti-atherosclerotic Effect of Alisma Rhizome Extracts
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초록
Objectives : The study was conducted to evaluate antioxidative, anti-atherosclerotic and anti-hypertensive effects of natural remedies. Alisma Rhizome (AR) has been used for a long time in Asia in folk remedies for treatment of hypertension and stroke and has been used in Korean traditional medicine for the treatment of glycosuria, gonorrhea, hypercholesterolemia, hypertension, and jaundice and its diuretic effect. These pharmacological effects of AR might come from antioxidant properties of phytochemicals in these materials. Methods : In this study, the antioxidant activity of extract from AR was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+ induced human LDL oxidation and on ACE. Results : The AR extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on ACE against all of the reactive species tested, with the water extract showing particularly strong antioxidant activities. Conculsions : The AR extracts have antioxidative, anti-atherosclerotic and anti-hypertensive effects in an in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.
Abstract
Objectives : The study was conducted to evaluate antioxidative, anti-atherosclerotic and anti-hypertensive effects of natural remedies. Alisma Rhizome (AR) has been used for a long time in Asia in folk remedies for treatment of hypertension and stroke and has been used in Korean traditional medicine for the treatment of glycosuria, gonorrhea, hypercholesterolemia, hypertension, and jaundice and its diuretic effect. These pharmacological effects of AR might come from antioxidant properties of phytochemicals in these materials. Methods : In this study, the antioxidant activity of extract from AR was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+ induced human LDL oxidation and on ACE. Results : The AR extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on ACE against all of the reactive species tested, with the water extract showing particularly strong antioxidant activities. Conculsions : The AR extracts have antioxidative, anti-atherosclerotic and anti-hypertensive effects in an in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.
참고문헌 (30)
- Sorg O., "Oxidative stress: a theoretical model or a biological reality?", Comptes Rendus Biologies, 327(7), pp.649~662 (2004)
- Perezs DD, "Wine, diet, antioxidant defense, and oxidative damage", Ann. N. Y. Acad. Sci, 957, pp.136~145 (2002)
- Raya, AA, "Inflammation: A pivotal link between autoimmune diseases and atherosclerosis", Autoimmunity, 5, pp.331~337 (2006)
- Libby P., "Inflammation in atherosclerosis", Nature, 420, pp.868~874 (2002)
- William OA, "Renin- angiotensin inhibitors. In Principles of Medical chemistry", pp.724~ (1995), William & Wilkins
- Samy IM, "Mechanisms by Which Angiotensin-Converting Enzyme Inhibitors Prevent Diabetes and Cardiovascular Disease", Am. J. Cardiol, 91, pp.30H~37H (2003)
- Brewster UC, "The renin-angiotensin- aldosterone system: cardiorenal effects and implications for renal and cardiovascular disease states", Am. J. Med. Sci, 326, pp.15~24 (2003)
- Zalba G, "Oxidative stress in arterial hypertension. Role of NAD(P)H oxidase", Hypertension, 38, pp.1395~1399 (2001)
- 한방약리학 교재편찬위원회, "한방약리학", pp.503~508 (2005), 신일상사
- Kim SE, "Antioxidant effect of Alisma plantago-aquatica var. orientale and its main component", Kor. J. Pharmacogn, 38(4), pp.372~375 (2007)
- Choi SS., "The effects of Alisima canaliculatum butanol fraction with selenium on glycogen level, lipid metabolism and lipid peroxidation in streptozotocon-induced diabetic rats", The Korean Nutrition Scociety, 37(1), pp.15~22 (2004)
- 한혜경, "택사 butanol 분획물과 vitamin E의 투여가 streptozotocin 유발 당뇨 흰쥐의 글리코겐, 지질함량 및 지질과산화에 미치는 영향A", 한국식품과학회지, 36(3), pp.465~471 (2004), 한국식품과학회
- Toh CA., "Antimicrobial and antifungal studies on Alismae Rhizoma", Kor. J. Pharmacogn, 27(4), pp.378~382 (1996)
- Delledonne M, "The functions of nitric oxide mediated signalling and changes in gene expression during the hypersensitive response", Antioxid. Redox Signal, 5, pp.33~41 (2003)
- Ali KA, "Tea and herbal infusions: Their antioxidant activity and phenolic propolis", Food Chemistry, 89, pp.27~36 (2005)
- Miller NJ, "A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates", Clin. Sci, 84, pp.407~412 (1993)
- Rivero MDP, "Role of melanoidins in sweet wines", Analytica Chimica Acta, 458, pp.169~175 (2002)
- Blois MS, "Antioxidant determination by the use of stable free radical", Nature, 26, pp.1199~1200 (1958)
- Yen GC, "Antioxidant activity and active compounds of rice koji fermented with Aspergillus candidus", Food Chemistry, 83, pp.49~54 (2003)
- Wassmann S, "Modulation of oxidant and antioxidant enzyme expression and function in vascular cells", Hypertension, 44, pp.381~386 (2004)
- Madamanchi NR, "Oxidative stress in atherogenesis and arterial thrombosis: the disconnect between cellular studies and clinical outcomes", Thrombo Haemost, 3, pp.254~267 (2004)
- Wang H, "Identification of polyphenols in tobacco leaf and theirantioxidant and antimicrobial activities", Food Chemistry, 107(4), pp.1399~1406 (2008)
- Brown JM, "Effects of amphetamines on mitochondrial function: role of free radicals and oxidative stress", Pharmacology & Therapeutics, 99, pp.45~53 (2003)
- Hogg N, Darley-UVM, "Production of hydroxyl radicals from the simultaneous generation of superoxide and nitric oxide", Biochem J, 281, pp.419~424 (1992)
- Raya AA, "Inflammation: A pivotal link between autoimmune diseases and atherosclerosis", Autoimmunity Reviews, 5, pp.31~337 (2006)
- Ryu BH, "Antioxidative effects of flavonoids toward modification of human low density lipoprotein", KOREAN J. FOOD & NUTR, 12(3), pp.320~327 (1999)
- Ondetti MA, "Design of specific inhibitors of angiotensin converting enzyme: New class orally active antihypertensive agents", Science, 196, pp.441~ (1997)
- Kwon JW, "Inhibitory effects on angiotensin-converting enzyme of Korean medical plants", J. Life Sci. & Nat. Res, 26, pp.84~91 (2005)
- Al-Qattan KK, "The antihypertensive effect of galic (Allium sativum) in the rat two-kidney one-clip Goldblatt model", J. Ethnopharmacol, pp.217~222 (1999)
- Inokuchi J, "Antihypertensive substance in seeds of Areca catechu L", Life sciences, 38, pp.1375~1382 (1986)