흰쥐에서 민들레 추출물이 사염화탄소에 의한 산화적 스트레스의 경감기전
Taraxacum Mongolicum H. Suppress Hepatoprotective Activity by Increasing Liver Antioxidant Enzyme in Carbon Tetrachloride(CCl4)-induced Hepatotoxicity in Rats
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Pretreatment with Taraxacum Mongolicum H(TMH) prior to the administration of on CCl4 significantly prevented the increased serum enzymatic activity of aminotransferase(ALT, AST), gamma-glutamyl transpeptidase(GGT) and bilirubin concentration in dose-dependent manner. In addition, pretreatment with TMH also significantly restored the elevation of hepatic malondialdehyde formation and the depletion of reduced glutathione content in the liver CCl4-intoxicated rats. The restoration of microsomal aniline hydroxylase and aminopyrine N-demethylase activities indicated the improvement in functional status of endoplasmic reticulum. CCl4-induced hepatotoxicity was also essentially prevented, as indicated by a liver histopathologic study. TMH showed antioxidant effects in FeCl2-ascorbate-induced lipid peroxidation in rat liver homogenate and in superoxide radical scavenging activity. Our results suggest that the protective effect of TMH against CCl4-induced hepatotoxicity possibly involve mechanisms related to its ability to block p450-mediated CCl4 bioactivation and free radical scavenging effects.
Abstract
Pretreatment with Taraxacum Mongolicum H(TMH) prior to the administration of on CCl4 significantly prevented the increased serum enzymatic activity of aminotransferase(ALT, AST), gamma-glutamyl transpeptidase(GGT) and bilirubin concentration in dose-dependent manner. In addition, pretreatment with TMH also significantly restored the elevation of hepatic malondialdehyde formation and the depletion of reduced glutathione content in the liver CCl4-intoxicated rats. The restoration of microsomal aniline hydroxylase and aminopyrine N-demethylase activities indicated the improvement in functional status of endoplasmic reticulum. CCl4-induced hepatotoxicity was also essentially prevented, as indicated by a liver histopathologic study. TMH showed antioxidant effects in FeCl2-ascorbate-induced lipid peroxidation in rat liver homogenate and in superoxide radical scavenging activity. Our results suggest that the protective effect of TMH against CCl4-induced hepatotoxicity possibly involve mechanisms related to its ability to block p450-mediated CCl4 bioactivation and free radical scavenging effects.
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