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랫드에서 thioacetamide의 반복 투여로 유도된 간 손상에 대한 민들레 추출물의 효과

Protective effects of dandelion extract against liver damage by repeated administration of thioacetamide in rats

정지영|김하정|신성식|손창호|오기석|서국현|문선진
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2017.06|한국동물위생학회지|제40권 제2호|pp. 107-117

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참고문헌

키워드

ThioacetamideTaraxacum officinaDandelion water extractAntioxidant enzymesLiver injuries
주제분류수의학

초록

This study was conducted to investigate of hepatoprotective effect of dandelion water extract (DWE) according to repeated administration of thioacetamide (TAA) induced hepatotoxicity in Spraque-Dawley rats. Thirty rats were randomly assigned to 5 groups; normal control, DWE-control, TAA-control (TAA injection during the feeding of normal diet), TAA&DWE600 (TAA repeated injection during the feeding of DWE 600 mg/kg BW), TAA&DWE1200 (TAA repeated injection during the feeding of DWE 1,200 mg/kg BW). Rats in DWE-control and TAA&DWE groups were treated with DWE (600 or 1,200 mg/kg BW daily) by gavage for 20 days (twice a day). All the rats in the TAA-control and TAA&DWE groups were repeated injection of TAA (100 mg/kg BW) into the abdominal cavity 3 days interval and 12 hrs later, all rats were sacrificed. At the same time, normal control and DWE-control groups were injected normal saline. In TAA&DWE groups, serum alanine and aspartate aminotransferase (ALT, AST) were significantly decreased and triglyceride (TG) synthesis was significantly increased compared to TAA group. As well as total billilubin and GGT were slightly decreased by the treatment of DWE. Lipid peroxidation (MDA) concentration was significantly decreased and hepatic GSH content was slightly or significantly increased in the TAA&DWE groups compared to TAA group. Hepatic anti-oxidative enzyme activities, such as GSH, GST, SOD and catalase were slightly or significantly elevated by the treatment of DWE. According to these results, When dandelion extract was long term supplied, it could be used as a potential protective material for a longer time liver damage by repeated adminstra-tion of the TAA.

Abstract

This study was conducted to investigate of hepatoprotective effect of dandelion water extract (DWE) according to repeated administration of thioacetamide (TAA) induced hepatotoxicity in Spraque-Dawley rats. Thirty rats were randomly assigned to 5 groups; normal control, DWE-control, TAA-control (TAA injection during the feeding of normal diet), TAA&DWE600 (TAA repeated injection during the feeding of DWE 600 mg/kg BW), TAA&DWE1200 (TAA repeated injection during the feeding of DWE 1,200 mg/kg BW). Rats in DWE-control and TAA&DWE groups were treated with DWE (600 or 1,200 mg/kg BW daily) by gavage for 20 days (twice a day). All the rats in the TAA-control and TAA&DWE groups were repeated injection of TAA (100 mg/kg BW) into the abdominal cavity 3 days interval and 12 hrs later, all rats were sacrificed. At the same time, normal control and DWE-control groups were injected normal saline. In TAA&DWE groups, serum alanine and aspartate aminotransferase (ALT, AST) were significantly decreased and triglyceride (TG) synthesis was significantly increased compared to TAA group. As well as total billilubin and GGT were slightly decreased by the treatment of DWE. Lipid peroxidation (MDA) concentration was significantly decreased and hepatic GSH content was slightly or significantly increased in the TAA&DWE groups compared to TAA group. Hepatic anti-oxidative enzyme activities, such as GSH, GST, SOD and catalase were slightly or significantly elevated by the treatment of DWE. According to these results, When dandelion extract was long term supplied, it could be used as a potential protective material for a longer time liver damage by repeated adminstra-tion of the TAA.

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관련 논문AI추천

Thioacetamide로 유발된 간 손상에 대한 Taraxacum officinale (Dandelion) 추출물의 효과마세령, 문선진, 서국현, 손창호, 신성식, 오기석, 유도현, 정영훈, 조인영, 최창용, 허태영한국동물위생학회 · 한국동물위생학회지2013 · 제36권 제4호흰쥐에서 민들레 추출물이 사염화탄소에 의한 산화적 스트레스의 경감기전김성훈, 최종원한의병리학회 · 동의생리병리학회지2010 · 제24권 제3호흰민들레(Taraxacum coreanum) 추출물이 급성 수은 중독된 생쥐의 간에 미치는 효과허진, 김종세, 노영복, 윤중식, 이현화, 정민주, 최영복한국현미경학회 · Applied Microscopy2008 · 제38권 제1호Thioacetamide 유도 간 독성 모델에서 맥문동 추출물의 보호 효과박서인, 백승화, 서희원, 오지혜, 황석연한의병리학회 · 동의생리병리학회지2025 · 제39권 제1호본초학적 접근을 통해 본 국내에서 연구된 한약재의 사염화탄소로 유발된 간손상에 대한 간보호 효과김나현, 김수일, 김진주, 신민규한의병리학회 · 동의생리병리학회지2011 · 제25권 제5호대황과 실리마린의 병용투여의 간섬유화 보호 효과노성수, 정일하, 지상우대한한방내과학회 · 대한한방내과학회지2023 · 제44권 제3호

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