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흑삼의 항산화 활성

Antioxidant Activity of Black Panax ginseng

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초록

This study was performed to investigate the antioxidant activity of extracts of black Panax ginseng (BGE) and its crude saponin (BGECS). The antioxidant activities of BGE and BGECS were evaluated for free radical scavenging activity against stable free radical (1,1-diphenyl-2-picrylhydrazyl) DPPH, nitrite, hydrogen peroxide and superoxide. In addition, the antioxidant activity of BGE and BGECS against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. As a result, BGE and BGECS were found to have a strong inhibitory activity with >90% against the DPPH radical at 1000 ㎍/㎖ concentrations. Also, BGE and BGECS exhibited strong inhibitory activity with >80% against hydrogen peroxide at lower concentration (125 ㎍/㎖). Moreover, specific TOSC values (405 and 473 TOSC/mM) of BGE and BGECS against peroxynitrites were higher than GSH (347 TOSC/mM) used a positive control. These results suggest that BGE and BGECS could be useful to develop functional foods against disease related oxidative stress.

Abstract

This study was performed to investigate the antioxidant activity of extracts of black Panax ginseng (BGE) and its crude saponin (BGECS). The antioxidant activities of BGE and BGECS were evaluated for free radical scavenging activity against stable free radical (1,1-diphenyl-2-picrylhydrazyl) DPPH, nitrite, hydrogen peroxide and superoxide. In addition, the antioxidant activity of BGE and BGECS against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. As a result, BGE and BGECS were found to have a strong inhibitory activity with >90% against the DPPH radical at 1000 ㎍/㎖ concentrations. Also, BGE and BGECS exhibited strong inhibitory activity with >80% against hydrogen peroxide at lower concentration (125 ㎍/㎖). Moreover, specific TOSC values (405 and 473 TOSC/mM) of BGE and BGECS against peroxynitrites were higher than GSH (347 TOSC/mM) used a positive control. These results suggest that BGE and BGECS could be useful to develop functional foods against disease related oxidative stress.

참고문헌 (32)

  1. 홍문화, "한국인삼사, 상권", pp.48~ (1980), 삼화인쇄주식회사
  2. 이상인, "한국인삼사. 하권", pp.166~ (1980), 삼화인쇄주식회사
  3. Park, J.D, "Recent studies on the chemical constituents of Korean ginseng", Korean J. Ginseng Sci, 20, pp.389~415 (2006)
  4. Sanata, S., "Studies on the saponins of ginseng. I. Structure of ginseng-R0, Rb1, Rb2, Rc and Rd", Chem. Pharm. Bull, 22, pp.421~428 (1974)
  5. Kitagawa, I., "Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2) ; Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root", Yakugaku Zasshi, 107, pp.495~505 (1987)
  6. Lee, D.C, "Effect of ether, ethanol and aqueous extracts of ginseng on cardiovascular function in dogs", Can. J. Comp. Med, 45, pp.182~187 (1981)
  7. Jie, Y.H., "Immunomodulatory effects of Panax ginseng C.A. Meyer in the mouse", Agents Actions, 15, pp.386~391 (1984)
  8. Kim, Y.C., "Ginsenosides Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration", J. Neurosci. Res, 53, pp.426~432 (1998)
  9. Joo, C.N, "The effects of ginseng saponins on alcohol dehydrogenase", Hanguk Saenghwa Hakhoechi, 10, pp.109~120 (1977)
  10. Tahara, M, "Action of ginsenosides on tumor cells. Growth inhibition and redifferentiation of neoplasia", Wakan Yaku Gakkaishi, 2, pp.170~171 (1985)
  11. Nguyen Huu Tung, "Dammarane-type Saponins from the Black Ginseng", Bulletin of the Korean Chemical Society, 31(11), pp.3423~3426 (2010), 대한화학회
  12. Mi-Ra Lee, "Effect of Black Ginseng on Memory Improvement in the Amnesic Mice Induced by Scopolamine", Journal of Ginseng Research, 34(1), pp.51~58 (2010), 고려인삼학회
  13. Ae-Jung Kim, "The Chemopreventive Potential and Anti-inflammatory Activities of Korean Black Ginseng in Colon26-M3.1 Carcinoma Cells and Macrophages", Journal of the Korean Society for Applied Biological Chemistry, 53(1), pp.101~105 (2010), 한국응용생명화학회
  14. Sun, B.S, "Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC-ELSD", Journal of pharmaceutical and biomedical analysis, 50(1), pp.15~22 (2009)
  15. Pellegrini, M., "Apoptosis and oxidative stress -related diseases: the p66 Shc connection", Current Molecular Medicine, 9(3), pp.392~398 (2009)
  16. Bayir, H, "Reactive oxygen species", Crit. Care Med, 3, pp.498~501 (2005)
  17. Malle, E., "Modification of low-density lipoprotein by myeloperoxidase-derived oxidants and reagent hypochlorous acid", Biochim. Biophys. Acta, 1761, pp.392~415 (2006)
  18. Kamat, J.P, "Peroxynitrite: a potent oxidizing and nitrating agent", Indian J. Exp. Biol, 44, pp.436~447 (2006)
  19. Nomura, T, "Proton-donative antioxidant activity of fucoxanthin with 1,1-diphenyl-2-picrylhydrazyl (DPPH)", Biochem Mol. Biol. Int, 42, pp.361~370 (1997)
  20. Regoli, F., "Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach", Mar. Environ. Res, 54, pp.419~423 (2002)
  21. Regoli, F., "Quantification of total oxidant scavenging capacity of antioxidants for peroxynitrite, peroxyl radicals, and hydroxyl radicals", Toxicol. Appl. Pharmacol, 156, pp.96~105 (1999)
  22. 이지현, "흑삼의 제조 및 항암효과", 동의생리병리학회지, 20(4), pp.951~956 (2006), 대한동의병리학회
  23. Blois, M.S, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  24. Kato, H, "Inhibition of nitrosamine formation by nondialyzable melanoidines", Agric. Biol. Chem, 51, pp.1333~1338 (1987)
  25. Okamura, H., "Antioxidant activity of tannins and flavonoids in Eucalptus rostrata", Phytochemistry, 33, pp.557~561 (1993)
  26. Park, S.W, "Active oxygen scavenging activity of luteolin-7-O-β-D-glucoside isolated from Humulus japonicus", Korean Soc Food Sci Nutr, 29, pp.106~110 (2000)
  27. Winston, G.W, "A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids", Free Radic. Biol. Med, 24, pp.480~493 (1998)
  28. Yokozawa, T., "Ginsenoside Rd attenuates oxidative damage related to aging in senescence-accelerated mice", J. Pharm. Pharmacol, 56(1), pp.107~113 (2004)
  29. Chun, H, "Free radical scavenging capacity as related to antioxidant activity and ginsenoside composition of asian and North american ginseng extracts", JAOCS, 78(3), pp.249~255 (2001)
  30. Liu, Z.Q., "In vitro study of the relationship between the structure of ginsenoside and its antioxidative or prooxidative activity in free radical induced hemolysis of human erythrocytes", J. Agric. Food Chem, 51(9), pp.2555~2558 (2003)
  31. Kang, K.S, "Study on the hydroxyl radical scavenging activity changes of ginseng and ginsenoside-Rb2 by heat processing", Biol. Pharm. Bull, 30(4), pp.724~728 (2007)
  32. Aruoma, O.I, "Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods", Mutat. Res, 524, pp.9~20 (2003)

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