흑인삼엽의 제조 및 항산화 활성 평가
Preparation of Black Panax ginseng Leaf and Evaluation of its Antioxidative Effect
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초록
The purpose of this study is to prepare black Panax ginseng leaf (PGL) and evaluate its antioxidative effect. In order to make black PGL, the raw PGL was successiely steamed at 95℃ for 3 hr nine times. The antioxidant activities of total saponins (Sa) from PGL and black PGL against peroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Specific TOSC values for black PGL-Sa against peroxyl radicals and peroxynitrites were 2.3-fold and 2.1-fold of PGL-Sa, respectively, and 2.2-fold and 5.2-fold of glutathione, a positive control antioxidant, respectively. The black PGL-Sa exhibited stronger antioxidative effect than PGL-Sa. The main ginsenosides of black PGL were Rg3, Rk1 and Rg5. Among the saponins in black PGL, the amount of ginsenoside Rg3 was examined by HPLC. 22.12 mg of ginsenoside Rg3 was obtained from 1g of dried black PGL.
Abstract
The purpose of this study is to prepare black Panax ginseng leaf (PGL) and evaluate its antioxidative effect. In order to make black PGL, the raw PGL was successiely steamed at 95℃ for 3 hr nine times. The antioxidant activities of total saponins (Sa) from PGL and black PGL against peroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Specific TOSC values for black PGL-Sa against peroxyl radicals and peroxynitrites were 2.3-fold and 2.1-fold of PGL-Sa, respectively, and 2.2-fold and 5.2-fold of glutathione, a positive control antioxidant, respectively. The black PGL-Sa exhibited stronger antioxidative effect than PGL-Sa. The main ginsenosides of black PGL were Rg3, Rk1 and Rg5. Among the saponins in black PGL, the amount of ginsenoside Rg3 was examined by HPLC. 22.12 mg of ginsenoside Rg3 was obtained from 1g of dried black PGL.
참고문헌 (16)
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