본문 바로가기
KERD
논문KCI 등재

자연산 산삼, 산양삼 및 인삼의 항산화능 비교연구

A study on the comparison of antioxidant effects among wild ginseng, cultivated wild ginseng, and cultivated ginseng extracts

2

조회

0

다운로드

28

인용

0

참고문헌

키워드

주제분류한의학

초록

Objectives: The objective of this study was to compare the antioxidant effects among wild ginseng, cultivated wild ginseng, and ginseng extracts. Methods: In vitro antioxidant activities were examined by total antioxidant capacity (TAC), oxygen radical scavenging capacity (ORAC), total phenolic content, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, inhibition of induced lipid peroxidation using liver mitochondria, reactive oxygen species (ROS) scavenging effect using 2',7'-dichlorofluorescein (DCF) fluorescence. Results: 1. TAC of 1.5 and 3.75 ㎎ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 2. ORAC of 2, 10, and 20 ㎍ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 3. Total phenolic content of 0.375, 0.938, and 1.875 ㎎ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 4. DPPH (1,1-Diphenyl-2-picrylhydrazyl) scavenging activity between wild ginseng and cultivated wild ginseng did not differ significantly (p>0.05). 5. Induced lipid peroxidation, measured by TBARS concentration in solution containing rat liver mitochondria incubated in the presence of FeSO4/ascorbic acid was inhibited as amounts of wild ginseng, cultivated wild ginseng, and ginseng extracts increased. TBARS concentration of ginseng extracts were significantly (p<0.05) higher than wild ginseng or cultivated wild ginseng extracts. 6. DCF fluorescence intensity was decreased as concentrations of wild ginseng, cultivated wild ginseng, and ginseng extracts increased, demonstrating that ROS generation was inhibited in a concentration-dependent manner. Conclusions: In summary, the results of this study demonstrate that cultivated wild ginseng extracts had similar antioxidant activities to wild ginseng extracts and greater that of cultivated ginseng extracts.

Abstract

Objectives: The objective of this study was to compare the antioxidant effects among wild ginseng, cultivated wild ginseng, and ginseng extracts. Methods: In vitro antioxidant activities were examined by total antioxidant capacity (TAC), oxygen radical scavenging capacity (ORAC), total phenolic content, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, inhibition of induced lipid peroxidation using liver mitochondria, reactive oxygen species (ROS) scavenging effect using 2',7'-dichlorofluorescein (DCF) fluorescence. Results: 1. TAC of 1.5 and 3.75 ㎎ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 2. ORAC of 2, 10, and 20 ㎍ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 3. Total phenolic content of 0.375, 0.938, and 1.875 ㎎ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 4. DPPH (1,1-Diphenyl-2-picrylhydrazyl) scavenging activity between wild ginseng and cultivated wild ginseng did not differ significantly (p>0.05). 5. Induced lipid peroxidation, measured by TBARS concentration in solution containing rat liver mitochondria incubated in the presence of FeSO4/ascorbic acid was inhibited as amounts of wild ginseng, cultivated wild ginseng, and ginseng extracts increased. TBARS concentration of ginseng extracts were significantly (p<0.05) higher than wild ginseng or cultivated wild ginseng extracts. 6. DCF fluorescence intensity was decreased as concentrations of wild ginseng, cultivated wild ginseng, and ginseng extracts increased, demonstrating that ROS generation was inhibited in a concentration-dependent manner. Conclusions: In summary, the results of this study demonstrate that cultivated wild ginseng extracts had similar antioxidant activities to wild ginseng extracts and greater that of cultivated ginseng extracts.

참고문헌 (41)

  1. M. Valko, "Free radicals and antioxidant in normal physiological funtions and human disease", The International J. & Cell Biology, 39, pp.44~84 (2007)
  2. Sawyer, DT., "How super is superoxide?", ACC. Chem. Res, 14, pp.393~ (1981)
  3. Fridorich, I., "Biological effects of the superoxide radical", Arch. Biophys, 247, pp.1~11 (1986)
  4. Ames, BN., "Dietary carcinogens and anticarcinogens", Oxygen radical and degenerative disease. Science, 221, pp.1256~1264 (1983)
  5. Chance, B., "Hydroperoxide metabolism in mammalian organs", Physiol. Rev, 59, pp.527~ (1979)
  6. Kandawami, C., "Free radical scavenging and anti-oxident activity of plant flavonoids. Free Radicals in Diagnostic Medicine", pp.351~376 (1994), Armstrong D. Plenum Press
  7. 하대유, "인삼에 대한 세포학 및 면역학적 연구", 대한 면역학회지, 1(1), pp.45~52 (1979)
  8. 山田昌之, "朝鮮人蔘의 硏究", 日本藥理學會誌, 51, pp.390~ (1955)
  9. Brekhman. I.I., "Panax ginseng" (1957), Gosudarst Isdat et Med, Lit. Leningard
  10. 최진호, "인삼의 신비", pp.13~14 (1984), 교문사
  11. 남기열, "최신 고려인삼" (1996), 천일인쇄소
  12. Takagi, K., "Proceedings International Ginseng Symposium, The Central Research Institute", Office of Monopoly, 119 (1974), Seoul
  13. 김승환, "인삼복용시 과산화지질과 총항산화능에 미치는 영향", 한국체육학회지, 42(3), pp.661~668 (2003), 한국체육학회
  14. Choi, KJ., "Effect of solvents on the yield, brown color intensity, UV absorbance, reducing and antioxident activities of extracts from white and red ginseng", J. Korean Agric. Chem. Soc, 26, pp.8~18 (1983)
  15. 이승은, "인삼의 부위별 항산화 활성", 한국약용작물학회지, 12(3), pp.237~242 (2004), 한국약용작물학회
  16. Hovius, R., "Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane", Biochim. Biophys. Acta, 1021, pp.217~226 (1990)
  17. Re, R., "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol. Med, 26, pp.1231~1237 (1999)
  18. Erel, O., "A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation", Clin. Biochem, 37, pp.277~285 (2004)
  19. Huang, D., "High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format", J. Agric. Food Chem, 50, pp.4437~4444 (2002)
  20. Singleton, V.L., "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", Methods Enzymol, 299, pp.152~178 (1999)
  21. Malterud, K.E., "Antioxidant and radical scavenging effects of anthraquinones and anthrones", Pharmacology, 47, pp.77~85 (1993)
  22. Stacey, N.H., "Inhibition of lipid peroxidation without prevention of cellular injury in isolated rat hepatocytes", Toxicol. Appl. Pharm, 58, pp.8~18 (1981)
  23. LeBel, C.P., "Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reative oxyten species formation and oxidative stress", Chem. Res. Toxicol, 5, pp.227~231 (1992)
  24. Lowry, O.H., "Protein measurements with the Folin phenol reagent", J. Biol. Chem, 193, pp.265~275 (1951)
  25. Devy, C., "New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase", Biochem. Pharmacol, 39, pp.399~405 (1990)
  26. Kuramoto, T., "Development and application of food materials from plant extracts such as SOD", Up to date Food Processing, 27, pp.22~23 (1992)
  27. Rorald, W., "Naturally occurring nitrite in food", J. Japan Soc. Food Agric, 26, pp.1735~1742 (1975)
  28. Jeon, HK., "Effects of ginseng sponin fraction and cyclophosphamide on the tumoricidal activity of mouse macrophage and antitumor effect", Korean J. Ginseng Sci, 15, pp.99~105 (1991)
  29. Kim, MJ., "The effects of ginseng saponin on the mouse immune system", Korean J. Ginseng Sci, 11, pp.130~135 (1987)
  30. Kang, SY., "The anti-hypersensitive effect of red ginseng saponin and the endothelium-derived vascular relaxation", Korean J. Ginseng Sci, 16, pp.175~182 (1992)
  31. Joo, CN., "Study on the hypoglycemic action of ginseng saponin on streptozotocin induced diabetic rats", Korean J. Ginseng Sci, 16, pp.190~197 (1991)
  32. Oliveira, ACC., "Protective effects of Panax ginseng on muscle injury and inflammation after eccentric exercise", Comparative Biochemistry and Physiology Part C, 130, pp.369~377 (2001)
  33. Kim, JS., "Screening of anti-oxidative components from red ginseng saponin", Korean J. Ginseng Sci, 20, pp.173~178 (1996)
  34. Ryu, GH., "Present status of red ginseng products and its manufacturing process", Food Industry and Nutrition, 8, pp.38~42 (2003)
  35. Kitagawa, I., "Chemical studies on crude drug processing. Ⅰ. On constituents of ginseng radix rubra (1)", Yakugaku Zasshi, 103, pp.612~622 (1983)
  36. Kim, KH., "Acidic polysaccharide from Panax ginseng, ginsan, induces Th1 cell and macrophage cytokines and generates LAK cells in synergy with rⅡ-2", Planta media, 64, pp.110~115 (1998)
  37. Lee, JW., "Function of the water soluble browning reaction products isolated from Korean red ginseng 2. Linoleic acid, Ox-brain autoxidant and Fe2+ ADP", NAD system, 2000, pp.24, 35~40
  38. 김성진, "山蔘, 長腦蔘, 人蔘의 抗癌效果에 대한 비교연구", 대한본초학회지, 19(2), pp.41~50 (2004), 대한본초학회
  39. 單書健, "神農本草經校證" (1988), 吉林科學技術出版社
  40. Yoshizawa, S., "Antitumor promoting activity of (-)-epigallocatechin gallate, the main constitutent of tannin in green tea", Phytother. Res, 1, pp.44~47 (1987)
  41. Fridovich, I., "Biological effects of the superoxide radical", Adv. Enzymol, 58, pp.62~ (1986)

관련 논문AI추천

이 간행물의 다른 논문 (11건)