Validation of a Multiplex Assay for Measuring Specific IgG4
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Purpose: Specific IgG4 (sIgG4) increases with allergen specific immunotherapy and may reflect a state of immune tolerance in food allergy. While ImmunoCAP® has been widely used to measure sIgG4 to a single allergen, PROTIATM Specific IgG4® has been designed as a multiplex assay for measuring sIgG4. This study sought to validate this assay in comparison to ImmunoCAP®. Materials and Methods: Measurements of sIgG4 were compared between PROTIATM Specific IgG4® and ImmunoCAP® using sera from 519 allergy patients (asthma: 114, allergic rhinitis: 318, food allergy: 146) with 731 paired tests. sIgG4 was measured against nine inhalant allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat dander, dog dander, birch pollen, oak pollen, ragweed pollen, mugwort pollen, and Alternaria alternata spores) and nine food allergens (egg white, casein, wheat, peanut, walnut, crab, shrimp, apple, and peach). Results: PROTIATM Specific IgG4® showed 95.6% agreement rate with ImmunoCAP® in the positivity comparison. For sIgG4 positivity to each individual allergen, an agreement rate of more than 84.8% was observed. In Cohen’s kappa analysis, these assays displayed substantial correlations [Cohen’s kappa coefficient (κ) ≥0.699], except for shrimp (κ=0.448). Furthermore, both assays displayed strong correlations in quantitative comparisons [correlation coefficients value (ρ) ≥0.8014], except for apple (ρ=0.6571, p=0.175). Serial dilution tests also showed consistency between the assays. Conclusion: PROTIATM Specific IgG4® showed high consistency with ImmunoCAP® in measuring sIgG4. This assay is applicable to various clinical fields, including allergen immunotherapy and food allergy.
Abstract
Purpose: Specific IgG4 (sIgG4) increases with allergen specific immunotherapy and may reflect a state of immune tolerance in food allergy. While ImmunoCAP® has been widely used to measure sIgG4 to a single allergen, PROTIATM Specific IgG4® has been designed as a multiplex assay for measuring sIgG4. This study sought to validate this assay in comparison to ImmunoCAP®. Materials and Methods: Measurements of sIgG4 were compared between PROTIATM Specific IgG4® and ImmunoCAP® using sera from 519 allergy patients (asthma: 114, allergic rhinitis: 318, food allergy: 146) with 731 paired tests. sIgG4 was measured against nine inhalant allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat dander, dog dander, birch pollen, oak pollen, ragweed pollen, mugwort pollen, and Alternaria alternata spores) and nine food allergens (egg white, casein, wheat, peanut, walnut, crab, shrimp, apple, and peach). Results: PROTIATM Specific IgG4® showed 95.6% agreement rate with ImmunoCAP® in the positivity comparison. For sIgG4 positivity to each individual allergen, an agreement rate of more than 84.8% was observed. In Cohen’s kappa analysis, these assays displayed substantial correlations [Cohen’s kappa coefficient (κ) ≥0.699], except for shrimp (κ=0.448). Furthermore, both assays displayed strong correlations in quantitative comparisons [correlation coefficients value (ρ) ≥0.8014], except for apple (ρ=0.6571, p=0.175). Serial dilution tests also showed consistency between the assays. Conclusion: PROTIATM Specific IgG4® showed high consistency with ImmunoCAP® in measuring sIgG4. This assay is applicable to various clinical fields, including allergen immunotherapy and food allergy.
참고문헌 (43)
- Schroeder HW Jr, "Structure and function of immunoglobulins", J Allergy Clin Immunol, 125(2 Suppl 2), pp.S41~S52 (2010)
- French MA, "Serum IgG subclass concentrations in healthy adults : a study using monoclonal antisera", Clin Exp Immunol, 56, pp.473~475 (1984)
- Aalberse R, "The role of IgG antibodies in allergy and immunotherapy", Allergy, 66(Suppl 95), pp.28~30 (2011)
- Nirula A, "What is IgG4? A review of the biology of a unique immunoglobulin subtype", Curr Opin Rheumatol, 23, pp.119~124 (2011)
- Schuurman J, "Normal human immunoglobulin G4 is bispecific : it has two different antigen-combining sites", Immunology, 97, pp.693~698 (1999)
- Davies AM, "Human IgG4 : a structural perspective", Immunol Rev, 268, pp.139~159 (2015)
- Djurup R, "IgG subclass antibody response in grass pollen-allergic patients undergoing specific immunotherapy. Prognostic value of serum IgG subclass antibody levels early in immunotherapy", Allergy, 39, pp.433~441 (1984)
- Noh G, "The clinical significance of food specific IgE", IgG4 in food specific atopic dermatitis, 2007, pp.18, 63~70
- Song Y, "Correlations between basophil activation, allergen-specific IgE with outcome and severity of oral food challenges", Ann Allergy Asthma Immunol, 114, pp.319~326 (2015)
- Shamji MH, "Biomarkers for monitoring clinical efficacy of allergen immunotherapy for allergic rhinoconjunctivitis and allergic asthma : an EAACI Position Paper", Allergy, 72, pp.1156~1173 (2017)
- Okamoto S, "Predictive value of IgE", IgG4 antibody ratio in children with egg allergy, 2012, pp.8, 9~
- Clayton F, "Eosinophilic esophagitis in adults is associated with IgG4and not mediated by IgE", Gastroenterology, 147, pp.602~609 (2014)
- Van Helden PM, "IgG subclasses of anti-FVIII antibodies during immune tolerance induction in patients with hemophilia A", Br J Haematol, 142, pp.644~652 (2008)
- Iizuka A, "Analysis of IgG heavy chain subclasses of alloantibodies to factor IX by crossed immunoelectrophoresis of factor IX using the intermediate gel technique", Br J Haematol, 53, pp.687~688 (1983)
- Van Schouwenburg PA, "IgG4 production against adalimumab during long term treatment of RA patients", J Clin Immunol, 32, pp.1000~1006 (2012)
- Adjobimey T, "Induction of immunoglobulin G4 in human filariasis : an indicator of immunoregulation", Ann Trop Med Parasitol, 104, pp.455~464 (2010)
- Stone JH, "IgG4-related disease", N Engl J Med, 366, pp.539~551 (2012)
- Landis JR, "The measurement of observer agreement for categorical data", Biometrics, 33, pp.159~174 (1977)
- Schober P, "Correlation coefficients : appropriate use and interpretation", Anesth Analg, 126, pp.1763~1768 (2018)
- Cox L, "Comparison of allergen immunotherapy practice patterns in the United States and Europe", Ann Allergy Asthma Immunol, 103, pp.451~459 (2009)
- Wan KS, "The critical role of allergen-specific IgE, IgG4 and IgA antibodies in the tolerance of IgE-mediated food sensitisation in primary school children", Food Agr Immunol, 23, pp.93~98 (2012)
- Van de Veen W, "Role of IgG4 in IgE-mediated allergic responses", J Allergy Clin Immunol, 138, pp.1434~1435 (2016)
- Tang ML, "Oral immunotherapy and tolerance induction in childhood", Pediatr Allergy Immunol, 24, pp.512~520 (2013)
- 이상철, "Clinical Features and Culprit Food Allergens of Korean Adult Food Allergy Patients: A Cross-Sectional Single-Institute Study", Allergy, Asthma & Immunology Research, 11(5), pp.723~735 (2019), 대한천식알레르기학회
- Wang J, "Management of the patient with multiple food allergies", Curr Allergy Asthma Rep, 10, pp.271~277 (2010)
- 김미애, "Pollen-Food Allergy Syndrome in Korean Pollinosis Patients: A Nationwide Survey", Allergy, Asthma & Immunology Research, 10(6), pp.648~661 (2018), 대한천식알레르기학회
- 김민지, "Clinical Manifestations and Risk Factors of Anaphylaxis in Pollen–Food Allergy Syndrome", Yonsei Medical Journal, 60(10), pp.960~968 (2019), 연세대학교의과대학[DOI]
- Wright BL, "Food-specific IgG4 is associated with eosinophilic esophagitis", J Allergy Clin Immunol, 137(2 Suppl), pp.Ab232~ (2016)
- Rosenberg CE, "Esophageal IgG4 levels correlate with histopathologic and transcriptomic features in eosinophilic esophagitis", Allergy, 73, pp.1892~1901 (2018)
- Wright BL, "Food-specific IgG4 is associated with eosinophilic esophagitis", J Allergy Clin Immunol, 138, pp.1190~1192 (2016)
- Kagalwalla AF, "Effect of six-food elimination diet on clinical and histologic outcomes in eosinophilic esophagitis", Clin Gastroenterol Hepatol, 4, pp.1097~1102 (2006)
- Ridolo E, "Eosinophilic esophagitis : which role for food and inhalant allergens", Asia Pac Allergy, 2, pp.237~241 (2012)
- Elhassan A, "Strong association of eosinophilic esophagitis and oral allergy syndrome : evidence suggestive of oral route of sensitization to common foodpollen allergens", Am J Gastroenterol, 110, pp.S705~ (2015)
- Lucendo AJ, "Relation between eosinophilic esophagitis and oral immunotherapy for food allergy : a systematic review with meta-analysis", Ann Allergy Asthma Immunol, 113, pp.624~629 (2014)
- Miehlke S, "Induction of eosinophilic esophagitis by sublingual pollen immunotherapy", Case Rep Gastroenterol, 7, pp.363~368 (2013)
- Xu WL, "Diagnostic performance of serum IgG4 level for IgG4-related disease : a meta-analysis", Sci Rep, 6, pp.32035~ (2016)
- Culver EL, "Increased IgG4 responses to multiple food and animal antigens indicate a polyclonal expansion and differentiation of pre-existing B cells in IgG4-related disease", Ann Rheum Dis, 74, pp.944~947 (2015)
- Rioux JD, "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis", Nat Genet, 39, pp.596~604 (2007)
- Levine A, "Crohn’s disease exclusion diet plus partial enteral nutrition induces sustained remission in a randomized controlled trial", Gastroenterology, 157, pp.440~450 (2019)
- Rajendran N, "Food-specific IgG4-guided exclusion diets improve symptoms in Crohn’s disease : a pilot study", Colorectal Dis, 13, pp.1009~1013 (2011)
- Shepherd SJ, "Dietary triggers of abdominal symptoms in patients with irritable bowel syndrome : randomized placebo-controlled evidence", Clin Gastroenterol Hepatol, 6, pp.765~771 (2008)
- Zar S, "Food-specific IgG4 antibody-guided exclusion diet improves symptoms and rectal compliance in irritable bowel syndrome", Scand J Gastroenterol, 40, pp.800~807 (2005)
- Gocki J, "Role of immunoglobulin G antibodies in diagnosis of food allergy", Postepy Dermatol Alergol, 33, pp.253~256 (2016)