본문 바로가기
KERD
논문KCI 등재

Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류병리학

초록

Background: In vitro detection of the allergen-specific IgE antibody (sIgE) is a useful tool for the diagnosis and treatment of allergies. Although multiple simultaneous allergen tests offer simple and low-cost screening methods, these platforms also have limitations with respect to multiplexibility and analytical performance. As an alternative assay platform, we developed and validated a microarray using allergen extracts that we termed “GOLD” chip. Methods: Serum samples of 150 allergic rhinitis patients were used in the study, and the diagnostic performance of the microarray was compared with that of AdvanSure (LG Life Sciences, Daejun, Korea) and ImmunoCAP (Phadia, Uppsala, Sweden). Standard IgE samples were used for the quantitative measurement of sIgEs. Results: The microarray-based assay showed excellent performance in the quantitative measurement of sIgEs, demonstrating a linear correlation within the range of sIgE concentrations tested. The limit of detection (LOD) was lower than 0.35 IU/mL, which is the current standard for the LOD cut-off. The assay also provided highly reproducible sets of data. The total agreement percentage of positive and negative calls was 92.2% compared with ImmunoCAP. Moreover, an outstanding correlation was observed between the microarray and the ImmunoCAP results, with Cohen’s kappa and Pearson correlation coefficient values of 0.80 and 0.79, respectively. Conclusions: The microarray-based in vitro diagnostic platform offers a sensitive, reproducible, and highly quantitative method to detect sIgEs. The results showed strong correlations with that of ImmunoCAP. These results suggest that the new allergen microarray can serve as a useful alternative to current screening platforms, ultimately becoming a first-line screening method.

Abstract

Background: In vitro detection of the allergen-specific IgE antibody (sIgE) is a useful tool for the diagnosis and treatment of allergies. Although multiple simultaneous allergen tests offer simple and low-cost screening methods, these platforms also have limitations with respect to multiplexibility and analytical performance. As an alternative assay platform, we developed and validated a microarray using allergen extracts that we termed “GOLD” chip. Methods: Serum samples of 150 allergic rhinitis patients were used in the study, and the diagnostic performance of the microarray was compared with that of AdvanSure (LG Life Sciences, Daejun, Korea) and ImmunoCAP (Phadia, Uppsala, Sweden). Standard IgE samples were used for the quantitative measurement of sIgEs. Results: The microarray-based assay showed excellent performance in the quantitative measurement of sIgEs, demonstrating a linear correlation within the range of sIgE concentrations tested. The limit of detection (LOD) was lower than 0.35 IU/mL, which is the current standard for the LOD cut-off. The assay also provided highly reproducible sets of data. The total agreement percentage of positive and negative calls was 92.2% compared with ImmunoCAP. Moreover, an outstanding correlation was observed between the microarray and the ImmunoCAP results, with Cohen’s kappa and Pearson correlation coefficient values of 0.80 and 0.79, respectively. Conclusions: The microarray-based in vitro diagnostic platform offers a sensitive, reproducible, and highly quantitative method to detect sIgEs. The results showed strong correlations with that of ImmunoCAP. These results suggest that the new allergen microarray can serve as a useful alternative to current screening platforms, ultimately becoming a first-line screening method.

참고문헌 (36)

  1. Heinzerling L, "The skin prick test - European standards", Clin Transl Allergy, 3, pp.3~ (2013)[DOI]
  2. Liccardi G, "Systemic reactions from skin testing: literature review", J Investig Allergol Clin Immunol, 16, pp.75~78 (2006)
  3. Ownby DR, "Allergy testing: in vivo versus in vitro", Pediatr Clin North Am, 35, pp.995~1009 (1988)[DOI]
  4. Berg TLO, "Allergy diagnosis with the radioallergosorbent test", J Allergy Clin Immunol, 54, pp.209~221 (1974)[DOI]
  5. Gleeson M, "A clinical evaluation in children of the Pharmacia ImmunoCAP system for inhalant allergens", Clin Exp Allergy, 26, pp.697~702 (1996)[DOI]
  6. Kelso JM, "Diagnostic performance characteristics of the standard Phadebas RAST, modified RAST, and Pharmacia CAP system versus skin testing", Ann Allergy, 67, pp.511~514 (1991)
  7. 박경희, "Comparison of Singleplex Specific IgE Detection Immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy", Annals of Laboratory Medicine, 38(1), pp.23~31 (2018), 대한진단검사의학회
  8. Banker DD, "Allergy diagnosis by Pharmacia Cap System", Indian J Med Sci, 53, pp.387~389 (1999)
  9. Han MK, "Optimization of critical factors affecting the performance of an allergen chip for the analysis of an allergen-specific human IgE in serum", Anal Sci, 23, pp.545~549 (2007)[DOI]
  10. Melioli G, "The IgE repertoire in children and adolescents resolved at component level: a cross-sectional study", Pediatr Allergy Immunol, 23, pp.433~440 (2012)[DOI]
  11. Rim JH, "Comparison and clinical utility evaluation of four multiple allergen simultaneous tests including two newly introduced fully automated analyzers", Pract Lab Med, 4, pp.50~61 (2016)[DOI]
  12. 박경희, "Comparison of the ImmunoCAP Assay and AdvanSureTM AlloScreen Advanced Multiplex Specific IgE Detection Assay", Yonsei Medical Journal, 58(4), pp.786~792 (2017), 연세대학교의과대학[DOI]
  13. Cho JH, "Correlation between skin-prick testing, individual specific IgE tests, and a multiallergen IgE assay for allergy detection in patients with chronic rhinitis", Am J Rhinol Allergy, 28, pp.388~391 (2014)[DOI]
  14. Templin MF, "Protein microarray technology", Trends Biotechnol, 20, pp.160~166 (2002)[DOI]
  15. 전현진, "Intein-mediated Protein Engineering for Biosensor Fabrication", BioChip Journal, 10(4), pp.277~287 (2016), 한국바이오칩학회[DOI]
  16. 박혜정, "A Nationwide Survey of Inhalant Allergens Sensitization and Levels of Indoor Major Allergens in Korea", Allergy, Asthma & Immunology Research, 6(3), pp.222~227 (2014), 대한천식알레르기학회
  17. 최준석, "Clinical Availability of Component-Resolved Diagnosis Using Microarray Technology in Atopic Dermatitis", Annals of Dermatology, 26(4), pp.437~446 (2014), 대한피부과학회
  18. 김지현, "Sensitization to Aeroallergens in Korean Children: A Populationbased Study in 2010", Journal of Korean Medical Science, 26(9), pp.1165~1172 (2011), 대한의학회
  19. Lebrun SJ, "Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE", J Immunol Methods, 300, pp.24~31 (2005)[DOI]
  20. Fall BI, "Microarrays for the screening of allergen-specific IgE in human serum", Anal Chem, 75, pp.556~562 (2003)[DOI]
  21. Tae-EunKim, "Quantitative Measurement of Serum Allergen-Specific IgE on Protein Chip", Experimental and Molecular Medicine, 34(2), pp.9~158 (2002), 생화학분자생물학회
  22. Wiltshire S, "Detection of multiple allergen-specific IgEs on microarrays by immunoassay with rolling circle amplification", Clin Chem, 46, pp.1990~1993 (2000)
  23. Jahn-Schmid B, "Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen-specific serum immunoglobulin E", Clin Exp Allergy, 33, pp.1443~1449 (2003)[DOI]
  24. Ornberg RL, "Western blot analysis with quantum dot fluorescence technology: a sensitive and quantitative method for multiplexed proteomics", Nat Methods, 2, pp.79~81 (2005)[DOI]
  25. Deinhofer K, "Microarrayed allergens for IgE profiling", Methods, 32, pp.249~254 (2004)[DOI]
  26. Kimiko T, "Usability of Fag e 2 ImmunoCAP in the diagnosis of buckwheat allergy", Arch Dermatol Res, 303, pp.635~642 (2011)[DOI]
  27. Lucassen R, "Evaluation of a novel rapid test system for the detection of allergic sensitization to Timothy grass pollen against established laboratory methods and skin prick test", J Allergy, 2010 (2010)
  28. Saarinen KM, "Diagnostic value of skin-prick and patch tests and serum eosinophil cationic protein and cow's milkspecific IgE in infants with cow's milk allergy", Clin Exp Allergy, 31, pp.423~429 (2001)[DOI]
  29. Grier TJ, "Laboratory methods for allergen extract analysis and quality control", Clin Rev Allergy Immunol, 21, pp.111~140 (2001)[DOI]
  30. Andri L, "Local nasal immunotherapy with extract in powder form is effective and safe in grass pollen rhinitis: a double-blind study", J Allergy Clin Immunol, 97, pp.34~41 (1996)[DOI]
  31. Wagenlehner FM, "Pollen extract for chronic prostatitis-chronic pelvic pain syndrome", Urol Clin North Am, 38, pp.285~292 (2011)[DOI]
  32. 정주현, "Comparison of Component-Resolved Diagnosis by Using Allergen Microarray With the Conventional Tests in Allergic Rhinitis Patients: The First Using in Korea", Clinical and Experimental Otorhinolaryngology, 8(4), pp.385~389 (2015), 대한이비인후과학회[DOI]
  33. Schuurman J, "Production of a mouse", human chimeric IgE monoclonal antibody to the house dust mite allergen Der p 2 and its use for the absolute quantification of allergen-specific IgE, 1997, pp.99, 545~550
  34. Lupinek C, "Advances in allergen-microarray technology for diagnosis and monitoring of allergy: the MeDALL allergen-chip", Methods, 66, pp.106~119 (2014)[DOI]
  35. Curin M, "Microarrayed dog, cat, and horse allergens show weak correlation between allergen-specific IgE and IgG responses", J Allergy Clin Immunol, 133, pp.918~921 (2014)[DOI]
  36. Pomponi D, "Detection of IgG and IgE reactivity to BP180 using the <TEX>$ISAC^{(R)}$<", TEX> microarray system, 2013, pp.168, 1205~1214

관련 논문AI추천

이 간행물의 다른 논문 (18건)