A fragmentation database of soyasaponins by liquid chromatography with a photodiode array detector and tandem mass spectrometry
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Oleanane-type triterpenoids exist as secondary metabolites in various plants. In particular, soyasaponin, an oleanane-type triterpenoid, is abundant in the hypocotyl of soybean, one of the most widely cultivated crops in the world. Depending on their chemical structure, soyasaponins are categorized as group A saponins or group DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins. The different soyasaponin chemical structures present different health functionalities and taste characteristics. However, conventional phenotype screening of soybean requires a substantial amount of time for functionality of soyasaponins. Therefore, we attempted to use liquid chromatography with a photodiode array detector and tandem mass spectrometry (LCPDA/ MS/MS) for accurately predicting the phenotype and chemical structure of soyasaponins in the hypocotyl of five common soybean natural mutants. In this method, the aglycones (soyasapogenol A [SS-A] and soyasapogenol B [SS-B]) were detected after acid hydrolysis. These results indicated that the base peak and fragmentation differ depending on the chemical structure of soyasaponin with aglycone. Thus, a fragmentation database can help predict the chemical structure of soyasaponins in soyfoods and plants.
Abstract
Oleanane-type triterpenoids exist as secondary metabolites in various plants. In particular, soyasaponin, an oleanane-type triterpenoid, is abundant in the hypocotyl of soybean, one of the most widely cultivated crops in the world. Depending on their chemical structure, soyasaponins are categorized as group A saponins or group DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins. The different soyasaponin chemical structures present different health functionalities and taste characteristics. However, conventional phenotype screening of soybean requires a substantial amount of time for functionality of soyasaponins. Therefore, we attempted to use liquid chromatography with a photodiode array detector and tandem mass spectrometry (LCPDA/ MS/MS) for accurately predicting the phenotype and chemical structure of soyasaponins in the hypocotyl of five common soybean natural mutants. In this method, the aglycones (soyasapogenol A [SS-A] and soyasapogenol B [SS-B]) were detected after acid hydrolysis. These results indicated that the base peak and fragmentation differ depending on the chemical structure of soyasaponin with aglycone. Thus, a fragmentation database can help predict the chemical structure of soyasaponins in soyfoods and plants.
참고문헌 (11)
- G. R. Fenwick, "In Toxic Substances in Crop Plants", pp.285~327 (1991), Woodhead Publishing
- W. A. Oleszek, , Journal of Chromatography A, 1112, pp.78~91 (2006)
- P. Krishnamurthy, , Bioscience, Biotechnology, and Biochemistry, 78(12), pp.1988~1996 (2014)
- P. Krishnamurthy, , Plant Cell Physiol, 60(5), pp.1082~1097 (2019)
- Xiaohang Wang, "Mass Spectrometry for Metabolome Analysis", Mass Spectrometry Letters, 11(2), pp.17~24 (2020), 사단법인 한국질량분석학회
- M. Shiraiwa, , Agric. Biol. Chem, 55, pp.323~331 (1991)
- M. Shiraiwa, , Agric. Biol. Chem, 55, pp.911~917 (1991)
- M. Shiraiwa, , Agric. Biol. Chem, 54, pp.1347~1352 (1990)
- T. Sayama, , The Plant Cell, 24, pp.2123~2138 (2012)
- S. Kudou, , Bioscience, Biotechnology, and Biochemistry, 56(1), pp.142~143 (1992)
- W. Zhang, , J. Agric. Food Chem, 57, pp.3620~3625 (2009)