Analysis of soyasaponin content and biosynthesis-related gene expression in young pea (Pisum sativum L.) sprouts
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In legumes, soyasaponins, one of triterpenoid saponins, are major components of secondary metabolites with a more diverse array of bioactive chemicals. Although the biosynthetic pathway of soyasaponins has been largely studied in soybean, the study on the soyasaponin contents and biosynthesis-related gene expression in pea (Pisum sativum L.) is poorly understood. Here, we found the accumulation of only soyasaponin Bb component in the sprouts of two Korean domestic pea cultivars (Dachung and Sachul). This pattern was consistent with our observation that increased expression of PsUGT73P2 and PsUGT91H4 genes, but not PsCYP72A69, could be responsible for bio- synthesis of only soyasaponin Bb in pea by examining their gene expression. However, gradual accumulation of soy- asaponin Bb at developmental stages was not consistent with the expression of PsUGT73P2 and PsUGT91H4, suggesting that the changes of their protein activities may affect the accumulation patterns of soyasaponin Bb. We also revealed that the increased expression levels of PsUGT73P2 and PsUGT91H4 during light to dark transition led to increase of soyasaponin Bb contents. Collectively, our results provided a molecular basis of metabolic engineering for enhancing useful soyasaponin Bb metabolites in Korean domestic pea cultivars.
Abstract
In legumes, soyasaponins, one of triterpenoid saponins, are major components of secondary metabolites with a more diverse array of bioactive chemicals. Although the biosynthetic pathway of soyasaponins has been largely studied in soybean, the study on the soyasaponin contents and biosynthesis-related gene expression in pea (Pisum sativum L.) is poorly understood. Here, we found the accumulation of only soyasaponin Bb component in the sprouts of two Korean domestic pea cultivars (Dachung and Sachul). This pattern was consistent with our observation that increased expression of PsUGT73P2 and PsUGT91H4 genes, but not PsCYP72A69, could be responsible for bio- synthesis of only soyasaponin Bb in pea by examining their gene expression. However, gradual accumulation of soy- asaponin Bb at developmental stages was not consistent with the expression of PsUGT73P2 and PsUGT91H4, suggesting that the changes of their protein activities may affect the accumulation patterns of soyasaponin Bb. We also revealed that the increased expression levels of PsUGT73P2 and PsUGT91H4 during light to dark transition led to increase of soyasaponin Bb contents. Collectively, our results provided a molecular basis of metabolic engineering for enhancing useful soyasaponin Bb metabolites in Korean domestic pea cultivars.
참고문헌 (16)
- Abe I, "Enzymatic Cyclization of Squalene and Oxidosqualene to Sterols and Triterpenes", Chem Rev, 93, pp.2189~2206 (1993)
- Aguilera Y, "Starch, functional properties, and microstructural character-istics in chickpea and lentil as affected by thermal processing", J Agric Food Chem, 57, pp.10682~10688 (2009)
- Arribas C, "The effect of extrusion on the bioactive compounds and antioxidant capacity of novel gluten-free expanded products based on carob fruit, pea and rice blends", Innov Food Sci Emerg Technol, 52, pp.100~107 (2019)
- Bowles D, "Glycosyltransferases : managers of small molecules", Curr Opin in Plant Biol, 8, pp.254~263 (2005)
- Choi CW, "Germinated soy germ with increased soyasaponin Ab improves BMP-2-induced bone formation and protects against in vivo bone loss in osteoporosis", Sci Rep, 8, pp.12970~ (2018)
- Guang CE, "Biological Functionality of Soyasaponins and Soyasapogenols", J Agric Food Chem, 62, pp.8247~8255 (2014)
- Hughes J, "Legumes-A Comprehensive Exploration of Global Food-Based Dietary Guidelines and Consumption", Nutrients, 14, pp.3080~ (2022)
- Lu ZX, "Composition, physicochemical properties of pea protein and its application in functional foods", Crit Rev Food Sci Nutr, 60, pp.2593~2605 (2020)
- Ma M, "Effects of UV-B radiation on the isoflavone accumulation and physiological-biochemical changes of soybean during germination Physiological-biochemical change of germinated soybean induced by UV-B", Food Chem, 250, pp.259~267 (2018)
- Messina MJ, "Soy intake and cancer risk : a review of the in vitro and in vivo data", Nutr Cancer, 21, pp.113~131 (1994)
- Mora-Escobedo R, "Effect of protein hydrolysates from germinated soybean on cancerous cells of the human cervix : an in vitro study", Plant Foods Hum Nutr, 64, pp.271~278 (2009)
- Moses T, "Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives", Crit Rev Biochem Mol Biol, 49, pp.439~462 (2014)
- Pedrosa MM, "Comparison of Bioactive Compounds Content and Techno-Functional Properties of Pea and Bean Flours and their Protein Isolates", Plant Foods Hum Nutr, 75, pp.642~650 (2020)
- Thimmappa R, "Triterpene Biosynthesis in Plants", Annu Rev Plant Biol, 65, pp.225~257 (2014)
- Wang FZ, "Isoflavone, gamma-aminobutyric acid contents and antioxidant activities are significantly increased during germination of three Chinese soybean cultivars", J Funct Foods, 14, pp.596~604 (2015)
- 윤영재, "Molecular analysis of soyasaponin biosynthetic genes in two soybean (Glycine max L. Merr.) cultivars", Plant Biotechnology Reports, 15(1), pp.117~124 (2021), 한국식물생명공학회[DOI]