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CGH를 이용한 한국인 자궁경부 편평상피세포암 세포주의 염색체 DNA 변이 분석

Analysis of chromosomal DNA aberrations in Korean cervical squamous cell carcinoma cell lines by CGH

박미혜|전선희|김승철|김종일|정지학|황인택|박준숙|박수연|오관영
대한산부인과학회 (Korean Soceity of Obstetrics and Gynecology)|2005.03|Obstetrics & Gynecology Science|제48권 제3호|pp. 597-606

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참고문헌

키워드

Comparative genomic hybridization (CGH)CGH (comparative genomic hybridization)염색체 DNA 변이자궁 경부 편평세포암Chromosomal DNA aberrationsCervical squamous cell carcinoma cell lines
주제분류산부인과학

초록

목적: 비교 유전자 부합법 (comparative genomic hybridization, CGH)은 일종의 새로운 FISH 방법으로 기존의 세포유전학적 분석으로는 용이하지 않았던 모든 염색체 상의 상대적인 염색체 변이를 한번에 알 수 있게 해준다. 이에 본 연구를 한국인의 자궁경부 편평상피 세포암 세포주를 이용해 염색체 DNA 변이를 분석해 보고자 하였다. 연구 방법: 본 연구에서는 한국인 자궁경부 편평상피세포암 세포주 SNU-17, SNU-682와 SNU-902를 대상으로 DNA를 추출한 후, CGH를 시행하여 자궁경부 편평상피세포암 발생과 관련된 염색체 변이를 밝히고자 하였다. 결과: 세 개의 세포주 모두에서 염색체 DNA gain으로 나타난 부위는 염색체 5p, 5q22-q23, 8q12, 14q21-qter와 20이었고, 세개의 세포주 모두에서 염색체 DNA loss로 나타난 부위는 21q21-qter이었다. 세포주 2개에서 공통적으로 발견된 염색체의 변이는, 3q, 6p, 7p13-pter, 9p22-pter, 9q21-qter, 15q21-q22, 17q22-qter, 18p11.3-pter, 18q11.2-q12, 19p13.3-pter, 19q13.2- q13.3와 22q12-qter의 염색체 DNA gain과 4p14-pter, 10p11.2-p13와 10q24의 염색체 DNA loss로 분석되었다. 결론: 이 연구에서 밝혀진 염색체 DNA 변이 부위는 앞으로 자궁경부 편평상피세포암의 발생 및 진행과 관련된 유전자를 규명하는데 중요한 기초자료로 이용될 것으로 기대된다.

Abstract

Objective: Comparative genomic hybridization (CGH) is a new fluorescence in situ hybridization (FISH) technique to identify genomic aberrations in cancers. The purpose of this study was to analyze non-random chromosomal DNA aberrations involved in cervical squamous cell carcinoma cell lines from Korean women. Methods: We analyzed non-random chromosomal DNA aberrations involved in cervical squamous cell carcinoma cell lines from Korean women, SNU-17, SNU-682, and SNU-902 using CGH. Results: Chromosomal DNA gains of 5p, 5q22-q23, 8q11.2-q12, 14q21-qter, and 20 as well as chromosomal DNA losses of 21 were found frequently. Chromosomal DNA gains on chromosome 3q, 6P, 7p13-pter, 9p22-pter, 9q21-qter, 15q21-q22, 17q22-qter, 18p11.3-pter, 18q11.2-q21, 19p13.3-pter, 19q13.2-q13.3, and 22q12-qter, with losses on 4p14-pter, 10p11.2-p13 and 10q24 were observed in 2 of 3 cell lines. Conclusion: Non-random aberrations which were disclosed in this study might be candidate regions for the abnormal genes involved in the tumorigenesis of cervical squamous cell carcinomas. Datas about chromosomal aberrations of Korean squamous cell carcinoma cell lines in this study could afford very useful basic information for the development of diagnostic and therapeutic modalties targeting the abnormal genes associated with uterine cervical cancer in Korea.

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Difference of Genome-Wide Copy Number Alterations between High-Grade Squamous Intraepithelial Lesions and Squamous Cell Carcinomas of the Uterine Cervix김수영, 김유임, 노상영, 이범희, 이아원대한병리학회 · Journal of Pathology and Translational Medicine2012 · 제46권 제2호자궁경부 상피내종양과 침윤성 자궁경부암에서의 Fhit와 p53 단백 발현양상명나혜, 박진완, 주선하대한산부인과학회 · Obstetrics & Gynecology Science2004 · 제47권 제12호DNA Gains and Losses of Chromosome in Laryngeal Squamous Cell Carcinoma Using Comparative Genomic HybridizationAhter D. Toraman, Gulay Ozbilim, Guven Luleci, Ibrahim Keser, Kenan Guney연세대학교의과대학 · Yonsei Medical Journal2008 · 제49권 제6호HPV 16 양성 자궁경부암 세포주에서cDNA Microarray에 의한 유전자 발현허걸, 강인수, 김영식, 김인선, 김혜옥, 박찬우, 임정화, 장향란, 조준형대한산부인과학회 · Obstetrics & Gynecology Science2003 · 제46권 제4호한국여성에서 자궁경부암의 고위험군 인유두종바이러스의 동정권혁찬, 박민혜, 윤효진, 이방현, 이병관, 이상희, 임정애대한산부인과학회 · Obstetrics & Gynecology Science2004 · 제47권 제12호침윤성 자궁경부암에서 Vascular Endothelial Growth Factor와 Angiopoietin-1 & -2 mRNA의 발현에 관한 연구김승철, 문혜성, 박한뫼, 안정자, 정혜원대한산부인과학회 · Obstetrics & Gynecology Science2004 · 제47권 제8호

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