침윤성 자궁경부암에서 Vascular Endothelial Growth Factor와 Angiopoietin-1 & -2 mRNA의 발현에 관한 연구
Vascular Endothelial Growth Factor and Angiopoietin-1 & -2 mRNA Expressions in Invasive Cervical Carcinoma
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목적 : 본 연구에서는 자궁경부암과 정상 자궁경부조직에서의 VEGF mRNA와 Ang-1 & -2 mRNA 발현을 연구함으로서, 자궁경부암의 암화과정에 관여하는지를 알아보고자 하였으며 VEGF mRNA와 Ang-1 & -2 mRNA발현의 차이를 알아보고 자궁경부암 예후인자와의 관련성을 알아보고자 하였다. 연구 방법 : 34예의 자궁경부암 환자와 14예의 정상자궁경부 조직에서 RT-PCR과 QC-PCR을 이용하여 VEGF mRNA와 Ang-1 & -2 mRNA 발현을 비교하였다. 결과 : 자궁경부암과 정상자궁조직에서 VEGF mRNA와 Ang-1 & -2 mRNA가 발현되었으며 자궁경부암에서 통계적으로 유의하게 높게 발현되었다 (p<0.05). VEGF mRNA는 임상병기, SCC-Ag 그리고 임파절 전이와 관련이 있었으며, Ang-2 mRNA도 임상병기, 종괴의 크기, SCC-Ag 농도 및 임파절 전이와 관련이 있었다. 또한 VEGF mRNA와 Ang-2 mRNA의 발현은 통계적으로 유의한 관련성이 있었다 (p<0.05). 결론 : VEGF와 Ang-1 & -2는 자궁경부암의 진행 및 전이와 관련이 되고, 특히 VEGF와 Ang-2 mRNA의 발현은 예후인자와 상관관계가 있고, 상호 발현이 통계적으로 유의한 관련성이 있으므로 VEGF와 Ang-2의 예후인자로서의 가능성을 생각해 볼 수 있었다.
Abstract
Objective : This study was aimed to investigate the vascular endothelial growth factor (VEGF) mRNA and angiopoietin (Ang) -1 & -2 mRNA expressions between cervical cancer and normal cervix, and to assess the relationships among their expression and other prognostic factors of invasive cervical cancer. Methods : The tissues were obtained from 34 patients with cervical cancer and 14 patients with normal cervix undergoing hysterectomy. Total RNA was extracted and reverse transcribed into cDNA. RT-PCR and QC-PCR was performed to evaluate VEGF and Ang-1 & -2 mRNA expressions. Clinicopathologic factors of cervical cancer were reviewed with the patient's charts and results were analyzed with Mann-Whitney U test, Spearman correlation test and logistic regression analysis. Results : VEGF, Ang-1 & -2 mRNA expression in cervical cancer was higher than that in normal cervix (p<0.05) and there were significant correlations between Ang-2 mRNA expression and VEGF mRNA expression in all stage of cervical cancer (p<0.05). A definite correlation was found between VEGF mRNA expression and clinical stage, SCC-Ag levels, lymph node metastasis of cervical cancer (p<0.05). Also, There was significant correlation among Ang-2 mRNA expression and clinical stage, tumor sizes, SCC-Ag levels, lymph node metastasis of cervical cancer (p<0.05). Ang-1 mRNA expression was significantly correlated with clinical stage of cervical cancer (p<0.05). Conclusion : The expression of VEGF mRNA, Ang-1 & -2 mRNA could be associated with the progression and metastasis of cervical cancer and might have a role as prognostic parameters in cervical cancer.
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