임신 중 비타민 C와 비타민 E 투여 후 임신부 정맥혈장과 제대정맥혈장내 지질과산화물, 항산화능 및 항산화 비타민 농도
Effect of vitamin C and E on the lipid peroxide, antioxidant ability, and antioxidant vitamin levels in maternal and umbilical venous plasma
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목적: 임신 중 비타민 C와 비타민 E 투여 후 만삭임신부 정맥혈장과 제대정맥혈장내 지질과산화물, 항산화능 및 항산화비타민 농도의 변화를 알아보고자 하였다. 연구 방법: 초임부 또는 이전 자간전증이나 만삭전 조기양막파열 기왕력이 있었던 임부에게 무작위로 임신 15주에서 20주 사이부터 만삭 (임신 37주 이후) 분만 전까지 하루에 비타민 C 1,000 mg 과 비타민 E 400 IU를 투여했던 실험군 20명과 투여하지 않았던 대조군 20명을 대상으로 하였다. 비타민 투여 전과 분만직전 임부의 상완정맥혈액과 분만 후 제대정맥혈액을 채취하였다. Thiobarbituric acid (TBA) 반응을 이용하여 지질과산화물 농도를 측정하였고 Cao 등의 방법으로 항산화능을 측정하였으며, high performance liquid chromatography (HPLC)를 이용하여 아스코르빈산, 요산, 레티놀, 알파-토코페롤, 감마-토코페롤 및 베타 카로텐 농도를 측정하였다. 결과: 실험군에서 비타민 투여 후 임부 혈장내 지질과산화물 농도는 감소하였고, 항산화능 농도와 알파-토코페롤 농도는 증가하였다. 실험군의 제대정맥 혈장내 농도는 대조군에 비해 지질과산화물 농도는 낮았고, 항산화능 농도와 알파-토코페롤 농도는 높았다. 결론: 임신 중 비타민 C와 비타민 E 섭취로 임신부 정맥혈장과 제대정맥혈장내 지질과산화물은 감소하고 항산화능은 증가하였으며, 비타민 C 농도는 변화가 없었지만 비타민 E 농도는 증가하였다. 이는 임신부 혈액내 산화제-항산화제 불균형을 초래하는 산화적 스트레스로 생기는 산과적 질환의 예방적 목적으로 비타민 섭취가 이용될 수 있으며 태아의 항산화능에도 영향을 줄 수 있으리라 사료된다.
Abstract
Objective: This controlled trial investigated the effect of supplementation with vitamins C and E on the lipid peroxide, antioxidant ability, and antioxidant vitamin levels in full term maternal and umbilical venous plasma. Methods: Forty pregnant women were randomized in this study. They were nulliparous or multiparous women with history of preeclampsia or preterm premature rupture of membranes (PPROM). Twenty women were given vitamin C (1,000 mg/day) and vitamin E (400 IU/day) from 15~20 gestational weeks to delivery. The others were not given, as a control group. Maternal venous blood was obtained before vitamin supplementation and just before delivery. Umbilical venous blood was obtained after delivery. Lipid peroxide level and oxygen-radical absorbance capacity (ORAC) were measured by thiobarbituric acid reaction and Cao's method, respectively. Ascorbic acid, uric acid, β-carotene, retinol, α-tocopherol, and γ-tocopherol were measured by high performance liquid chromatography. Results: Supplementation with vitamins C and E was associated with decrease in lipid peroxide levels and increase in ORAC values and α-tocopherol levels in maternal venous plasma. And it was also associated with decrease in lipid peroxide levels and increase in ORAC values and α-tocopherol levels in umbilical venous plasma. Conclusion: Supplementation with vitamins C and E may be beneficial in the prevention of oxidant-antioxidant imbalance origin diseases such as preeclampsia, PPROM. And it also may affect antioxidant ability of the fetus.
참고문헌 (48)
- Berg CJ, "Pregnancy-related mortality in the United States, 1987-1990", Obstet Gynecol, 88, pp.161~167 (1996)
- Dekker GA, "Etiology and pathogenesis of preeclampsia: Current concepts", Am J Obstet Gynecol, 179, pp.1359~1375 (1998)
- VanWijk MJ, "Vascular function in preeclampsia", Cardiovasc Res, 47, pp.38~48 (2000)
- Hubel CA, "Oxidative stress in the pathogenesis of preeclampsia", Proc Soc Exp Biol Med, 222, pp.222~235 (1999)
- Hubel CA, "Lipid peroxidation in pregnancy: New perspectives on preeclampsia", Am J Obstet Gynecol, 161, pp.1025~1034 (1989)
- Roberts JM, "Clinical and biochemical evidence of endothelial cell dysfunction in the pregnancy syndrome preeclampsia", Am J Hypertens, 4, pp.700~708 (1991)
- Roberts JM, "Pre-eclampsia: more than pregnancy-induced hypertension", Lancet, 341, pp.1447~1451 (1993)
- Many A, "Hyperuricemia and xanthine oxidase in preeclampsia, revisited", Am J Obstet Gynecol, 174, pp.288~291 (1996)
- Patrono C, "Isoprostanes: potential markers of oxidant stress in atherothrombotic disease", Arterioscler Thromb Vasc Biol, 17, pp.2309~2315 (1997)
- Chappell LC, "Effect of antioxidants on the occurrence of pre-eclampsia in women at increased risk: a randomised trial", Lancet, 354, pp.810~816 (1999)
- Chappell LC, "Vitamin C and E supplementation in women at risk of preeclampsia is associated with changes in indices of oxidative stress and placental function", Am J Obstet Gynecol, 187, pp.777~784 (2002)
- Yoon BH, "Association of oligohydramnios in women with preterm premature rupture of membranes with an inflammatory response in fetal amniotic and maternal compartments", Am J Obstet Gynecol, 181, pp.784~788 (1999)
- Goldenberg RL, "The preterm prediction study: the value of new vs standard risk factors in predicting early and all spontaneous preterm births. NICHD MFMU Network", Am J Public Health, 88, pp.233~238 (1998)
- Cox SM, "International delivery versus expectant management with preterm ruptured membranes at 30-34 weeks' gestation", Obstet Gynecol, 86, pp.875~879 (1995)
- Arias F, "Etiology and outcome of low birth weight and preterm infants", Obstet Gynecol, 60, pp.277~281 (1982)
- Romero R, "Infection and labor. Ⅴ. Prevalence, microbiology, and clinical significance of intraamniotic infection in women with preterm labor and intact membranes", Am J Obstet Gynecol, 161, pp.817~824 (1989)
- Fiscella K, "Race, perinatal outcome, and amniotic infection", Obstet Gynecol Surv, 51, pp.60~66 (1996)
- Fortunato SJ, "Distinct molecular events suggest different pathways for preterm labor and premature rupture of membranes", Am J Obstet Gynecol, 184, pp.1399~1405 (2001)
- Robles R, "Oxidative stress in the neonate", Early Hum Dev, 65, pp.S75~S81 (2001)
- Tejero E, "Collagen synthesis during pregnancy, vitamin availability, and risk of preterm premature rupture of the membmranes", Int J Gynecol Obstet, 81, pp.29~34 (2003)
- Inder TE, "The correlation of elevated levels of an index of lipid peroxidation (MDA-TBA) with adverse outcome in the very low birth weight infant", Acta Paediatr, 85, pp.1116~1122 (1996)
- Levine GN, "Ascorbic acid reverses endothelial vasomotor dysfunction in patients with coronary artery disease", Circulation, 93, pp.1107~1113 (1996)
- Levine M, "Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance", Proc Natl Acad Sci U S A, 93, pp.3704~3709 (1996)
- Devaraj S, "Dose-response comparison of RRR-alpha-tocopherol and all-racemic alpha-tocopherol on LDL oxidation", Arterioscler Thromb Vasc Biol, 17, pp.2273~2279 (1997)
- Stephens NG, "Randomised controlled trial of vitamin E in patients with coronary disease: Cambridge Heart Antioxidant Study (CHAOS)", Lancet, 347, pp.781~786 (1996)
- Packer JE, "Direct observation of a free radical interaction between vitamin E and vitamin C", Nature, 278, pp.737~738 (1979)
- Chan AC, "Partners in defense, vitamin E and vitamin C", Can J Physiol Pharmacol, 71, pp.725~731 (1993)
- Ohkawa H, "Assay for lipid peroxidases in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Cao G, "Oxygen-radical absorbance capacity assay for antioxidants", Free Radic Biol Med, 14, pp.303~311 (1993)
- Johnson L, "Severe retinopathy of prematurity in infants with birth weights less than 1250 grams: incidence and outcome of treatment with pharmacologic serum levels of vitamin E in addition to cryotherapy from 1985 to 1991", J Pediatr, 127, pp.632~639 (1995)
- Mino M, "Fetal and maternal relationship in serum vitamin E level", J Nutr Sci Vitaminol (Tokyo), 19, pp.475~482 (1973)
- Draper D, "Elevated protease activities in human amnion and chorion correlate with preterm premature rupture of membranes", Am J Obstet Gynecol, 173, pp.1506~1512 (1995)
- Barrett BM, "Potential role of ascorbic acid and beta-carotene in the prevention of preterm rupture of fetal membranes", Int J Vitam Nutr Res, 64, pp.192~197 (1994)
- Breimer LH, "Molecular mechanisms of oxygen radical carcinogens and mutagenesis. The role of DNA base damage", Mol Carcinog, 3, pp.188~197 (1990)
- Michaelis J, "Different effects of hypochlorous acid on human neutrophil metalloproteinases: activation of collagenase and inactivation of collagenase and gelatinase", Arch Biochem Biophys, 292, pp.555~562 (1992)
- Halliwell B, "Role of free radicals and catalytic metal ions in human disease: an overview", Methods Enzymol, 186, pp.1~85 (1990)
- Plessinger MA, "Pretreatment of human amnion-chorion with vitamins C and E prevents hypochlorous acid-induced damage", Am J Obstet Gynecol, 183, pp.979~985 (2000)
- Woods JR Jr, "Vitamins C and E: missing links in preventing preterm premature rupture of membranes?", Am J Obstet Gynecol, 185, pp.5~10 (2001)
- Sedar Z, "Placental and decidual lipid peroxidation and antioxidant defenses in preeclampsia. Lipid peroxidation in preeclampsia", Pathophysiology, 9, pp.21~ (2002)
- Moretti M, "Increased breath markers of oxidative stress in normal pregnancy and in preeclampsia", Am J Obstet Gynecol, 190, pp.1187~1190 (2004)
- Brosens IA, "The role of the spiral arteries in the pathogenesis of preeclampsia", Obstet Gynecol Annu, 1, pp.177~191 (1972)
- Roberts JM, "Is oxidative stress the link in the two-stage model of pre-eclampsia?", Lancet, 354, pp.788~789 (1999)
- Knock GA, "Bradykinin-mediated relaxation of isolated maternal resistance arteries in normal pregnancy and preeclampsia", Am J Obstet Gynecol, 175, pp.1668~1674 (1996)
- Hubel CA, "Increased ascorbate radical formation and ascorbate depletion in plasma from women with preeclampsia: implications for oxidative stress", Free Rad Biol Med, 23, pp.597~609 (1997)
- Knekt P, "Epidemiology of vitamin E. Evidence for anticancer effects in humans. in: Vitamin E in health and disease", pp.513~527 (1993), Dekker
- Gülmezoğlu AM, "Antioxidants in the treatment of severe preeclampsia: an explanatory randomized controlled trial", Br J Obstet Gynaecol, 104, pp.689~696 (1997)
- Chappell LC, "Effect of antioxidants on the occurrence of preeclampsia in women at increased risk: a randomized trial", Lancet, 354, pp.810~816 (1999)
- Chappell LC, "Vitamin C and E supplementation in women at risk of preeclampsia is associated with changes in indices of oxidative stress and placental function", Am J Obstet Gynecol, 187, pp.777~784 (2002)