만삭 전자간증 산모의 양수에서 산화 스트레스 표지자인 단백질 카르보닐의 증가
Increased protein carbonyls as markers for oxidative stress in amniotic fluid of preeclamptic women at term
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=연구목적: 전자간증의 주요 발병기전으로 알려진 내피세포 기능장애를 유발하는 산화 스트레스의 표지자로서 단백질 카르보닐이 만삭 전자간증 산모의 양수에서 정상 산모에 비해 증가되어 있는지 알아보고자 하였다.연구방법: 만삭의 전자간증 또는 만성 고혈압 산모와 정상 만삭 산모의 양수 내 단백질 카르보닐을 비교하기 위하여 환자 대조군 연구를 수행하였다. 단백질 카르보닐은 특이 면역분석법으로 측정하였고, 통계적인 분석은 비모수적인 방법을 사용하였다.결과: 1) 만삭 전자간증 산모의 양수 내 단백질 카르보닐의 중앙값은 정상군보다 유의하게 높았다 (중앙값 0.663 nmol/mg, 범위 0.013-1.726 nmol/mg vs. 중앙값 0.395 nmol/mg, 범위 0.069-1.503 nmol/mg; p<.05). 2) 만삭 만성 고혈압 산모의 양수 내 단백질 카르보닐의 중앙값은 정상군과 차이가 없었다 (중앙값 0.823 nmol/mg, 범위 0.064-1.575 nmol/mg vs. 중앙값 0.395 nmol/mg, 범위 0.069-1.503 nmol/mg; p=.14). 3) 양수 내 단백질 카르보닐의 중앙값은 전자간증의 중증도에 따라 증가하는 경향을 보였으나 통계적으로 유의한 차이는 없었고 (정상군 중앙값 0.395 nmol/mg, 범위 0.069-1.503 nmol/mg; 경증 전자간증 중앙값 0.663 nmol/mg, 범위 0.013-1.054 nmol/mg; 중증 전자간증 중앙값 0.842 nmol/mg, 범위 0.391-1.726 nmol/mg; p=.07), 중증 전자간증 산모의 양수 내 단백질 카르보닐의 중앙값은 정상군보다 유의하게 높았다 (중앙값 0.842 nmol/mg, 범위 0.391-1.726 nmol/mg vs. 중앙값 0.395 nmol/mg, 범위 0.069-1.503 nmol/mg; p<.05).
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