만삭전 조기양막파열 임부에서 정맥혈장내 지질과산화도와 단백산화도의 증가
Increase in the lipid peroxidation and protein oxidation in the venous plasma of women with preterm pemature rupture of membranes
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=목적: 비임부, 정상 및 만삭전 조기양막파열 임부의 정맥혈장내 지질과산화 치와 단백산화촉진능의 차이를 비교하고자 한다. 연구방법: 비임부 20명, 임신 25주 이상 37주 미만의 정상 임부 20명과 조기양막파열 임부 20명으로부터 팔오금 중간정맥혈을 채취하였다. Thiobarbituric acid 반응을 이용하여 지질과산화물 농도를 측정하였다. 그리고 2,4-Dinitrophenylhydrazine (DNPH) 방법을 이용하여 단백질의 carbonyl기 함량의 기준치와 moxalactam 부치 후 및 amoxaciilin 부치 후의 단백질의 carbonyl기 함량을 측정하였다. 결과: 1. 만삭전 조기양막파열 임부군의 정맥혈장내 지질과산화 치는 5.66±0.43 nmol/mg protein으로 비임부군 3.78±0.24 nmol/mg protein, 정상 임부군 3.56±0.30 nmol/mg protein보다 의의있게 높았다 (p<0.01). 2. 만삭전 조기양막파열 임부군의 정맥혈장내 carbonyl기 함량도 8.23±0.54 nmol/mg protein으로 비임부군 6.39±0.23 nmol/mg protein, 정상 임부군 6.54±0.24 nmol/mg protein보다 의의있게 높았다 (p<0.01). 3. Moxalactam과 함께 부치시켰을 때 생성되는 만삭전 조기양막파열 임부군의 정맥혈장내 carbonyl기 함량은 11.73±0.59 nmol/mg protein으로 비임부군 10.06±0.26 nmol/mg protein, 정상 임부군 10.10±0.22 nmol/mg protein보다 의의 있게 높았다 (p<0.05). 4. Amoxacillin과 함께 부치시켰을 때 생성되는 정맥혈장내 carbonyl기 함량은 서로 간에 유의한 차가 없었다 (5.63±0.41, 5.81±0.43, 5.81±0.39 nmol/mg protein). 5. 정맥혈장내 지질과산화물 농도가 증가할수록 moxalactam에 부치 후 단백질의 carbonyl기 함량이 증가하는 비례적인 관계를 보였다 (p<0.05).
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