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세신고가 알레르기성 비염에 미치는 효과

The anti-allergic effect of SESHINGO(SSG)

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Objective : The purpose of this study was find out the anti-allergic effects of SESHINGO on the allergic rhinitis. Methods : Cell viability was evaluated by the MTT assay. Cells were treated with the indicated concentration of SSG. TNF-α concentration was measured from cell supernatants using ELISA method. IL-4 concentration was measured from cell supernatants using ELISA method.IFN-γ concentration was measured from cell supernatants using ELISA method. Total RNA was isolated, TNF-α and IL-4 mRNA expression was detected by RT-PCR analysis Total RNA was isolated, COX-1, COX-2 mRNA was analyzed by RT-PCR analysis. Results : 1. β-hexosaminidase release in RBL-2H3 cells were decresed by SESHINGO attentively . 2. Secreting ration of TNF-α was restrained in RBL-2H3 cells by SESHINGO attentively. 3. Secreting ration of IL-4 was restrained in RBL-2H3 cells by SESHINGO attentively 4. Revelation of TNF-α mRNA was decresed in RBL-2H3 cells as concetration. 5. Revelation of.IL-4 mRNA was decresed in RBL-2H3 cells as concetration. 6. Revelation of COX-2 mRNA was decresed in RBL-2H3 cells. Conclusion : According to above results, to evaluate the effect of SSG on the anti-allergic effects.

Abstract

Objective : The purpose of this study was find out the anti-allergic effects of SESHINGO on the allergic rhinitis. Methods : Cell viability was evaluated by the MTT assay. Cells were treated with the indicated concentration of SSG. TNF-α concentration was measured from cell supernatants using ELISA method. IL-4 concentration was measured from cell supernatants using ELISA method.IFN-γ concentration was measured from cell supernatants using ELISA method. Total RNA was isolated, TNF-α and IL-4 mRNA expression was detected by RT-PCR analysis Total RNA was isolated, COX-1, COX-2 mRNA was analyzed by RT-PCR analysis. Results : 1. β-hexosaminidase release in RBL-2H3 cells were decresed by SESHINGO attentively . 2. Secreting ration of TNF-α was restrained in RBL-2H3 cells by SESHINGO attentively. 3. Secreting ration of IL-4 was restrained in RBL-2H3 cells by SESHINGO attentively 4. Revelation of TNF-α mRNA was decresed in RBL-2H3 cells as concetration. 5. Revelation of.IL-4 mRNA was decresed in RBL-2H3 cells as concetration. 6. Revelation of COX-2 mRNA was decresed in RBL-2H3 cells. Conclusion : According to above results, to evaluate the effect of SSG on the anti-allergic effects.

참고문헌 (69)

  1. Pawankar R, "Allergic rhinitis and asthma: the link, the new ARIA classification and global approaches to treatment", Curr Opin Allergy Clin Immunol, 4, pp.1~4 (2004)
  2. 失數道明, "漢方診療醫典", pp.291~2 (1971), 高文社
  3. Datti F, "Influence of chronic unpredictable stress on the allergic responses in rats", Physiol Behav, 77, pp.79~83 (2002)
  4. Schmid-Ott G, "Different expres sion of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison with healthy controls", J Allergy Clin Immunol, 108, pp.455~62 (2001)
  5. Schmid-Ott G, "Levels of circulating CD8+ T lymphocytes, natural killer cells, and eosinophils increase upon acute psychosocial stress in patients with atopic dermatitis", J Allergy ClinImmunol, 107, pp.171~7 (2001)
  6. Chrousos GP, "Stress, chronic inflammation, and emotional and physical well-being: Concurrent. effects and chronic sequelae", Allergy ClinImmunol, 106, pp.S275~91 (2000)
  7. Theoharides TC, "The role of mast cells in migraine pathophysiology", Brain Res Rev, 49, pp.65~76 (2005)
  8. Wamboldt MZ, "Adolescents with atopic disorders have an attenuated corticol response to laboratory stress", J Allergy Clin Immunol, 111, pp.509~14 (2003)
  9. Schleimer RP, "Interactions between thehypothalamic-pituitary-adrenal axis and allergic inflammation", J Allergy Clin Immunol, 106, pp.S270~4 (2000)
  10. 양재하, "Allergy성 비염에 대한 양 ․한방적 고찰", 경산대학교 한의과대 학교부설 제한동의학술원 논문집, 4(1), pp.436~48 (1999)
  11. 강선미, "알레르기선 비염 면역반응에서의 Interleukin-12 작용효과", 소아 알레르기 및 호흡기학회지, 11(3), pp.213~23 (2001)
  12. 염승철, "알레르기비염 치료의 최신경향에 관한 동서의학적 고찰", 선무학술논집, 17(17), pp.191~214 (2007), 국제선무학회
  13. 허준, "동의보감", pp.591~ (1999), 동의보감 출판사
  14. 이상곤, "신한방이비인후과", pp.151~3 (2007), 정담출판사
  15. 강호건, "RBL-2H3 세포에서 백선피의 항알레르기 효과", 한방안이비인후피부과학회지, 20(1), pp.201~208 (2007), 대한한방안이비인후피부과학회
  16. 조주흠, "RBL-2H3 세포서 포공영의 항알레르기 효과", 20(1), pp.29~17 (2007)
  17. Schwartz LB, "Immunologic release of-hexosaminidase from purifiedrat serosal mast cells", Journal of Immunology, 123, pp.1445~50 (1979)
  18. 蔡炳允, "東醫眼耳鼻咽喉科學", pp.290~331 (1997), 서울:集文堂
  19. 劉完素, "河間三六書", pp.275~6 (1976), 서울. 성보사
  20. 張梅芳, "眼科與耳鼻咽喉科專病中醫臨床診治", pp.410~39 (2000), 北京. 人民衛生出版社
  21. 강기영, "임상 알레르기학", pp.10~9 (1984), 서울:여문각
  22. 王凍應, "中醫耳鼻咽喉科學", pp.132~6 (1993), 北京:科學出版社
  23. 박진구, "여택통기탕과 여택통기탕가미방의 항 알레르기 효과에 대한 실험적연구", 동의학회지, 5(1), pp.76~97 (2001)
  24. 이태헌, "細辛散의 항알레르기 효과에 관한 실험적 연구", 동의학회지, 4(1), pp.70~85 (2000)
  25. 최은성, "辛夷散과 辛夷散加味方의 항알레르기 효과에 대한 실험적 연구", 동의학회지, 3(1), pp.31~47 (1999)
  26. 윤근찬, "蒼耳散이 진통, 소염, 해열, 수면시간 및 히스타민 치사에 미치는 영향", 경희한의대논문집, 9, pp.401~10 (1986)
  27. 황학수, "알레르기성 비염 동물모델에서截敏蜜梅湯이 비염치료에 미치는 영향", 대한안이비인후피부과학회지, 16(3), pp.116~28 (2003)
  28. 이상문, "알레르기성 비염 흰쥐모델에서 理中湯合敗毒散이 비염치료에 미치는 영향", 한방안이비인후피부과학회지, 17(2), pp.72~80 (2004)
  29. 박진미, "加味桂枝湯이 알레르기 비염에 미치는 효과에 대한 연구", 한방안이비인후피부과학회지, 18(1), pp.234~240 (2005), 대한한방안이비인후피부과학회
  30. 심성용, "加味桂枝湯이 알레르기 비염에 미치는 영향에 관한 실험적 연구", 한방안이비인후피부과학회지, 17(1), pp.45~54 (2004)
  31. 박진미, "加味桂枝湯이 알레르기 비염에 미치는 효과에 대한 연구", 한방안이비인후피부과학회지, 18(1), pp.234~240 (2005), 대한한방안이비인후피부과학회
  32. 박주호, "小靑龍湯이 알레르기 비염에 미치는 효과에 대한 실험적 연구", 한방안이비인후피부과학회지, 17(1), pp.55~65 (2004)
  33. 송생엽, "小靑龍湯이 알레르기 비염 유발 백서의 과립구 및 조직학적 변에 미치는 효과", 한방안이비인후피부과학회지, 17(1), pp.75~81 (2004)
  34. 김종성, "補中益氣湯이 알레르기 비염 유발 白鼠의 과립구 및 조직학적 변화에 미치는 효과", 한방안이비인후피부과학회지, 17(1), pp.66~74 (2004)
  35. 박봉규, "十全大補湯이 알레르기 반응에 미치는 영향", 동의생리병리학회지, 17(2), pp.308~15 (2002)
  36. 김희정, "알러지성 비염 병태 모델에 대한 防風通聖散의 항알러지 효과", 한방안이비인후피부과학회지, 19(1), pp.21~30 (2006), 대한한방안이비인후피부과학회
  37. 양희연, "去痰補肺湯이 알레르기성 비염 모델 생쥐의 cytokine 변화에 미치는 영향", 한방안이비인후피부과학회지, 18(2), pp.28~35 (2005), 대한한방안이비인후피부과학회
  38. 정세연, "去痰補肺湯이 알레르기비염 모델 흰쥐에 미치는 영향", pp.2005
  39. 정진영, "通竅湯이 알레르기 비염 모델 흰 쥐에 미치는 영향", 한방안이비인후피부과학회지, 18(2), pp.36~50 (2005), 대한한방안이비인후피부과학회
  40. 천승철, "通竅湯加味方을 투여하여 치료한 알레르기성비염환자의 만족도에 대한 임상보고", 대한안이비인후피부과학회지, 15(2), pp.145~55 (2002)
  41. 박종원, "通關散이 알레르기성 비염 동물모델의 과립구 및 조직학적 변화에 미치는 영향", 석사, 한의학과, pp.2006
  42. 채병윤, "알레르기성 비염에 이용되는 通關散의藥效學的 硏究", 대한한의학회지, 11(1), pp.165~79 (1990)
  43. 채병윤, "생약복합제제약효연구 通關散의 效能에 대하여", 생약학회지, 21(2), pp.163~72 (1990)
  44. 전국한의과대학, "본초학교실", pp.135~455 (2000), 영림사
  45. Seldin DC, "Homology of the rat basophilic leukemia cell and the rat mucosal mast cell", Proc Natl Acad Sci, 82, pp.3871~5 (1985)
  46. Eccleston E, "Basophilic leukemia in the albino rat and a demonstration of the basopoietin", Nat New Biol, 244(133), pp.73~ (1973)
  47. Siraganian RP, "Mast cells and basophils, Inflammation: basic principles and clinical correlates", pp.513~ (1988), New York: Raven Press
  48. Kawakami, T, "Regulation of mast cell and basophil function and survival by IgE", Nature Reviews Immunology, 2, pp.773~86 (2002)
  49. Kay, A.B, "Allergy and allergic diseases", New England Journal of Medicine, 344(a), pp.30~7 (2001)
  50. Kay, A.B, "Allergy and allergic diseases", New England Journal of Medicine, 344(b), pp.109~13 (2001)
  51. Lee JH, "Inhibitory activity of Chrysanthemi sibirici herba extract on RBL-2H3 mast cells and compound 48", 80-induced anaphylaxis, 2004, pp.95, 425~30
  52. Marquardt DL, "Mast cells in allergic diseases and mastocytosis", West J Med, 37(3), pp.195~212 (1982)
  53. Razin E, "IgE-mediated release of leukotriene C4, chondroitin sulfate E proteoglycan, beta-hexosaminidase, and histamine from cultured bone marrow-derived mouse mast cells", J Exp Med, 157, pp.189~ (1983)
  54. Abassi YA, "Label-free, real-time monitoring of IgE-mediated mast cell activation on microelectronic cell sensor array", J Immuno Methods, 292, pp.195~205 (2004)
  55. Lee JH, "Inhibitory activity of Chrysanthemi sibirici herba extract on RBL-2H3 mast cells and compound 48", 80-induced anaphylaxis, 2004, pp.95, 425~30
  56. Bradding, P, "Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines", American Journal of Respiratory Cell and Molecular Biology, 10, pp.471~80 (1994)
  57. Ji-Sook Lee, "Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response", Journal of Ethnopharmacology, 117, pp.34~40 (2008)
  58. Baumgartner, R.A, "Secretion of TNF-α from a rat mast cell line is a brefeldin A-sensitive and a calcium", protein kinase C-regulated process, 1994, pp.153, 2609~17
  59. Phil-Dong Moon, "Use of scopoletin to inhibit the production of inf lammatory cytokines through inhibition of the Iκ B", NF-κB signal cascade in the human mast cell line HMC-1, 2007, pp.555, 218~25
  60. Abbas AK, "Functional diversity of helper T lymphocytes. Nature (Lond). 383:787-793, 1996; Paul WE. Interleukin-4: a prototypic immunoregulatory lyphokine", Blood, 77, pp.1859~70 (1991)
  61. Ji-Sook Lee, "Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response", Journal of Ethnopharmacology, 117, pp.34~40 (2008)
  62. Heijink, I.H, "Targeting T cells for asthma", Current Opinion in Pharmacology, 5, pp.227~31 (2005)
  63. Homey, B, "Cytokines and chemokines orchestrate atopic skin inflammation", Journal of Allergy and Clinical Immunology, 118, pp.178~89 (2006)
  64. Chatila, T.A, "Interleukin-4 receptor signaling pathways in asthma pathogenesis Interleukin-4 receptor signaling pathways in asthma pathogenesis", Trends in Molecular Medicine, 10, pp.493~9 (2004)
  65. Ji-Sook Lee, "Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response", Journal of Ethnopharmacology, 117, pp.34~40 (2008)
  66. Hata AM, "Pharmacology and signaling of prostaglandin receptors: Multiple roles in inflammation and immune modulation", Pharmacology & Threapeutics, 103, pp.147~66 (2004)
  67. Mitchell JA, "Cyclooxygenase-2: regulation and relevance inflammation", Biochem. Pharmacol, 50, pp.1535~ (1995)
  68. Herschman HR, "Prostaglandin synthase 2", Biochem. Biophys. Acta, 1299, pp.125~ (1996)
  69. Guan Z, "Induction of cyclooxygenase-2 by the activated MEKK-1 → SEK1", MKK4 → p38 mitogen-activated protein kinase pathway, 1998, pp.273, 12901~

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