升葛湯의 제형변화에 따른 항알레르기 효과의 비교 연구
The Comparative Study on the Difference of Anti-allergic Effects Based on Different form of Seunggal-tang
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Objectives Seunggal-tang is one of the prescriptions of oriental herbal medicine, which has been applied to several allergic diseases such as atopic dermatitis. This study was planned to compare differences of anti-allergic effects based on different form of Seunggal-tang by manufacturing differently. Methods Two types herb medicine products were used; aqueous extract (SG-T, Seunggal-Tang) and powder (SG-S, Seunggal-San) which were made from the same mixed formula of Seunggal-tang. To investigate in vitro anti-allergic activities, rat basophilic leukemia (RBL-2H3) cells were treated with SG-T and SG-S for 1 hour, and then stimulated with the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187. We examined the release of beta-hexosaminidase, as a marker of degranulation, and the releases of tumor necrosis factor (TNF)-alpha and interleukin (IL)-4, as proinflammatory cytokines. Results SG-T and SG-S didn’t have effects on cell viabilities in concentrations under 2㎎/㎖. In additionto that, SG-T more inhibited releasing β-hexosaminidase, TNF-α and IL-4 than SG-S. Conclusions These results indicate that SG-T is more effective against mast cell-mediated allergic reactions than SG-S.
Abstract
Objectives Seunggal-tang is one of the prescriptions of oriental herbal medicine, which has been applied to several allergic diseases such as atopic dermatitis. This study was planned to compare differences of anti-allergic effects based on different form of Seunggal-tang by manufacturing differently. Methods Two types herb medicine products were used; aqueous extract (SG-T, Seunggal-Tang) and powder (SG-S, Seunggal-San) which were made from the same mixed formula of Seunggal-tang. To investigate in vitro anti-allergic activities, rat basophilic leukemia (RBL-2H3) cells were treated with SG-T and SG-S for 1 hour, and then stimulated with the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187. We examined the release of beta-hexosaminidase, as a marker of degranulation, and the releases of tumor necrosis factor (TNF)-alpha and interleukin (IL)-4, as proinflammatory cytokines. Results SG-T and SG-S didn’t have effects on cell viabilities in concentrations under 2㎎/㎖. In additionto that, SG-T more inhibited releasing β-hexosaminidase, TNF-α and IL-4 than SG-S. Conclusions These results indicate that SG-T is more effective against mast cell-mediated allergic reactions than SG-S.
참고문헌 (22)
- 동의학연구소, "동의학개론", pp.333~ (1994), 여강출판사
- 한약제제 제조공정 표준화 사업(연구보고서), "이진영", 1~ (2005)
- Skehan P, "Assays of cell growth and cytotoxicity In: Cell Growth and Apoptosis", pp.180~ (1998), Oxford University press
- Schwartz L.B., "Immunologic release of β-hexosaminidasefrom purified rat serosal mast cells", Journal of Immunology, 123, pp.1445~1450 (1979)
- Key A.B, "Allergy and allergic diseases", N. Engl. J. Med, 344, pp.0~7 (2001)
- 강병수, "한방임상알러지", pp.22~23 (2002), 성보사
- 서울대학교의과대학, "면역학", pp.1~3 (1994), 서울대학교출판부
- Saimah Arif, "Immune, blood and lymphati system", pp.431~436 (2000), 한우리
- 고백화, "실용중의외과학", pp.443~444 (1985), 상해과학기술출판사
- 김미정, "소아 아토피 피부염에 대한 문헌적 고찰", 대한한방소아과학회지, 14, pp.167~182 (2000)
- 전을, "小兒藥證直訣", pp.90~ (1982), 강소과학기술출판사
- 김남권, "승마갈근탕가미방이 마우스의 항 allergy 및 면역반응에 미치는 영향", 대한외관과학회지, 8(1), pp.1~19 (1995)
- Ju BH, "The Study of Seunggal-tang Gamibang on the Anti-Allergy Effects", J Korean Oriental Peditrics, 21(2), pp.109~119 (2007)
- 윤정원, "두드러기 치료조사", 대한안이비인후피부과학회지, 16(2), pp.152~176 (2003)
- 윤소원, "승마갈근탕가미방을 사용한 두드러기 환자에 대한 임상연구", 대한안이비인후피부과학회지, 17(3), pp.61~79 (2004)
- 신상호, "升葛湯을 사용한 아토피 피부염 환자에 대한 임상시험", 한방안이비인후피부과학회지, 20(2), pp.199~212 (2007), 대한한방안이비인후피부과학회
- 김남재, "한방약물의 약리작용", 대한병원약사회지, 12(2), pp.121~138 (1995)
- 김남재, "한약과 양약의 병용투여에 의한 약물상호작용", 대한병원약사회지, 15(2), pp.247~257 (1998)
- Moon PD, "Inhibitory effect of Gamibojungikaitang extract on mast cell-mediated allergic reaction in murine Model", J Pharm Pharm Sci, 8(1), pp.94~101 (2005)
- Ennis M, "Some studies on the release of histamine from mast cells stimulated with polylysine", Br. J. Pharmacol, 70, pp.329~334 (1980)
- 이중달, "그림으로 설명한 병리학", pp.29~ (1991), 고려의학
- 정해영, "생명과학․분자의학을 위한 사이토카인 분자 생물학", pp.117~124 (2002), 월드사이언스