PMA와 A23187로 자극된 RBL-2H3 세포에 미치는 黃連解毒湯의 영향
Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA Plus A23187-stimulated RBL-2H3 Cells
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The purpose of this study was to investigate Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA plus A23187-stimulated RBL-2H3 cells. We examined MTT assay, β-hexosaminidase activity, IL-4 and TNF-α from RBL-2H3 after pre-treatment with HHT. In addition, we investigated expression of COX-1 and COX-2 mRNA using RT-PCR study. The results are as follows: All the concentrations of HHT from 1 to 4 ㎎/㎖ didn't have an effect on cell viability. In RBL-2H3 cells, β-hexosaminidase release, IL-4 and TNF-α were significantly reduced by treated all concentrations of HHT. COX-2 on mRNA expression was reduced at treatment 2mg of HHT prior to stimulating by A23187. These results indicate that HHT has inhibition effects on allergy in this study.
Abstract
The purpose of this study was to investigate Anti-allergic Inflammatory Effects of Hwangyounhaedog-tang on PMA plus A23187-stimulated RBL-2H3 cells. We examined MTT assay, β-hexosaminidase activity, IL-4 and TNF-α from RBL-2H3 after pre-treatment with HHT. In addition, we investigated expression of COX-1 and COX-2 mRNA using RT-PCR study. The results are as follows: All the concentrations of HHT from 1 to 4 ㎎/㎖ didn't have an effect on cell viability. In RBL-2H3 cells, β-hexosaminidase release, IL-4 and TNF-α were significantly reduced by treated all concentrations of HHT. COX-2 on mRNA expression was reduced at treatment 2mg of HHT prior to stimulating by A23187. These results indicate that HHT has inhibition effects on allergy in this study.
참고문헌 (25)
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