DL-HGF를 주성분으로 한 ethosome 및 liposome 제형화합물이 발모 촉진 활성에 미치는 영향
The Effects of Ethosome and Liposome Formulation Entrapped DL-HGF to Hair Growth Activity in Animal Model
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Objectives : To investigate the effects of DL-HGF to hair growth activity in mouse, various kinds of ethosome and liposome formulations entrapped DL-HGF were produced and this study was carried out. Methods : The B16/BL6 mice were classified into five groups: vehicle control (Con) group, Et-1-applied group, Et-2-applied group, LP-1-applied group, LP-2-applied group. Active hair growth (anagen) was induced in the back skin by application of a wax–osin mixture with subsequent depilation and the activity of hair growth was measured by macroscopic observation and histology. Results : In vehicle control group, there was no hair growth activity during experiment period. In Et-1-applied group, the rate of hair growth was about 100%, LP-1-applied group and Et-2-applied group showed 70-80% and 40-50% of hair growth rates, respectively. The rate of hair growth of LP-2-applied group was lower than other applied groups (20-30%). In H/E staining, Numerous hair folicles and hair shafts were observed in Et-1-applied group and other groups showed lower level of hair folicles and hair shafts formation than Et-1-applied group. Conclusion : Et-1 formulation showed highest hair growth activity than other ethosome and liposome formulations entrapped DL-HGF.
Abstract
Objectives : To investigate the effects of DL-HGF to hair growth activity in mouse, various kinds of ethosome and liposome formulations entrapped DL-HGF were produced and this study was carried out. Methods : The B16/BL6 mice were classified into five groups: vehicle control (Con) group, Et-1-applied group, Et-2-applied group, LP-1-applied group, LP-2-applied group. Active hair growth (anagen) was induced in the back skin by application of a wax–osin mixture with subsequent depilation and the activity of hair growth was measured by macroscopic observation and histology. Results : In vehicle control group, there was no hair growth activity during experiment period. In Et-1-applied group, the rate of hair growth was about 100%, LP-1-applied group and Et-2-applied group showed 70-80% and 40-50% of hair growth rates, respectively. The rate of hair growth of LP-2-applied group was lower than other applied groups (20-30%). In H/E staining, Numerous hair folicles and hair shafts were observed in Et-1-applied group and other groups showed lower level of hair folicles and hair shafts formation than Et-1-applied group. Conclusion : Et-1 formulation showed highest hair growth activity than other ethosome and liposome formulations entrapped DL-HGF.
참고문헌 (28)
- 李元文, "名醫臨證經驗", pp.572~622 (2001), 人民衛生出版社
- 楊思澍, "中醫臨床大全", pp.91~ (1988), 대성출판사
- 陣貴廷, "實用中醫結合診斷治療學", pp.1508~ (1992), 일중사
- 金達鎬, "黃帝內徑注解補注素問, 素問·陰陽應象 大論", pp.128~ (2001), 의성당
- 金達鎬, "黃帝內徑注解補注素問, 素問·痿論", pp.908~ (2001), 의성당
- 임사비나, "탈모에 대한 동서의 학적 고찰", 경산대학교 제한동의학술원 논문집, 4, pp.699~710 (1999)
- 고재승, "인체발생학", pp.483~ (1998), 정문각
- 金達鎬, "黃帝內徑注解補注素問, 素問·上古天眞論", pp.14~ (2001), 의성당
- 金達鎬, "黃帝內徑注解補注素問, 素問·上古天眞論", pp.18~ (2001), 의성당
- 金達鎬, "黃帝內徑注解補注素問, 素問·五藏生成論", pp.236~ (2001), 의성당
- 葛洪, "肘後備急方, 中國醫學大系", pp.500~ (1995), 여강출판사
- 丁光迪, "諸病源候論校注", pp.763~772 (1991), 人民衛生出版社
- Kirjavainen M, "Liposome-skin interactions and their effects on the skin permeation of drugs", Eur. J. Pharm. Sci, 7, pp.279~286 (1999)
- Egbaria K, "Topical application of liposomally entrapped cyclosporin evaluated by in vitro diffusion studies with human skin", Skin Pharmacol. Phys, 4, pp.21~28 (1991)
- Knepp V. M, "Transdermal drug delivery: problems and possibilities", Crit. Rev. Ther. Drug Carrier Syst, 4, pp.13~37 (1987)
- Visor G. C, "Quantitative evaluation of the stability and delivery of interleukin-1B by infusion", J. Parenter. Sci. Technol, 44, pp.130~132 (1990)
- Planas M. E, "Noninvasive percutaneous induction of topical analgesia by a new type of drug carrier, and prolongation of local pain insensitivity by nesthetic liposomes", Anesth. Analg, 75, pp.615~621 (1992)
- Cevc G, "Lipid vesicles penetrate into intact skin owing to the transdermal osmotic gradients and hydration force", Biochim. Biophys. Acta, 1104, pp.226~232 (1992)
- Heim M, "Lateral electrical conductivity of mica-supported lipid bilayer membranes measured by scanning tunneling microscopy", Biophys. J, 69, pp.489~497 (1995)
- Cevc G, "Transfersomes, liposomes and other lipid suspensions on the skin: permeation enhancement, vesicle penetration, and transdermal drug delivery", Crit. Rev. Ther. Drug Carrier Syst, 13, pp.257~388 (1996)
- Seddon J. M, "Phosphatidylcholine-fatty acid membranes: effects of headgroup hydration on the phase behaviour and structural parameters of the gel and inverse hexagonal (H(II)) phases", Biochim. Biophys. Acta., 1327, pp.131~147 (1997)
- Paul A, , Eur. J. Immunol, 25, pp.3521~3524 (1995)
- Trotta M, "Elastic liposomes for skin delivery of dipotassium glycyrrhizinate", Int. J. Pharm, 241, pp.319~327 (2002)
- Trotta M, "Hydrophilic microspheres from water-in-oil emulsions by the water diffusion technique", Pharm. Res, 21, pp.1445~1449 (2004)
- Touitou E, "Ethosomes-novel vesicular carriers for enhanced delivery: characterization and skin penetration properties", J. Control. Release, 65, pp.403~418 (2000)
- Dayan N, "Carriers for skin delivery of trihexyphenidyl HCl: ethosomes vs. liposomes", Biomaterials, 21, pp.1879~1885 (2000)
- Touitou E, "Methods for quantitative determination of drug localized in the skin", J. Control. Relea, 56, pp.7~21 (1998)
- Perrett S, "A simple method for the preparation of liposomes for pharmaceutical applications: characterization of the liposomes", J. Pharm. Pharmacol, 43, pp.154~161 (1991)