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C57BL/6N 마우스 탈모모델에서 미세다륜침과 영지추출물이 모발성장에 미치는 영향

Effects of Ganoderma Lucidum Extract Ethanol Extract and Microneedle Therapy System on Hair Growth in an Alopecia Model of C57BL/6N Mice

이승연|강경화|유선애|주봉현
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2014.05|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제28권 제2호|pp. 72-87

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참고문헌

키워드

AlopeciaMicroneedle therapy systemC57BL/6NGanoderma lucidum extract
주제분류한의학

초록

ObjectivesThis study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedletherapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice. MethodsFive-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTSand GLE + MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determinedphotographically, the hair density, thickness and length were identified by Folliscope and the weights of body andorgans were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzedby RT - PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining. ResultsThe promotion of hair growth was observed in GLE + MTS and MTS compared to CON. The hair density,thickness and length were also improved in GLE + MTS and MTS compared to CON. The mRNA expression ofTGF-α, TGF-β1, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE + MTSand MTS compared to CON. The hair follicles and hair root growth were improved in GLE + MTS and MTScompared to CON. In the above results, GLE + MTS were more effective than MTS. ConclusionsThese results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment ofalopecia.

Abstract

ObjectivesThis study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedletherapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice. MethodsFive-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTSand GLE + MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determinedphotographically, the hair density, thickness and length were identified by Folliscope and the weights of body andorgans were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzedby RT - PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining. ResultsThe promotion of hair growth was observed in GLE + MTS and MTS compared to CON. The hair density,thickness and length were also improved in GLE + MTS and MTS compared to CON. The mRNA expression ofTGF-α, TGF-β1, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE + MTSand MTS compared to CON. The hair follicles and hair root growth were improved in GLE + MTS and MTScompared to CON. In the above results, GLE + MTS were more effective than MTS. ConclusionsThese results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment ofalopecia.

참고문헌 (36)

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