蕎麥의 비만세포 염증매개물질의 분비와 FcεRI 신호전달에 미치는 효과
Fagopyrum esculentum Extract Suppresses the Release of Inflammatory Mediator and Proximal Signal Events in FcεRI-mediated RBL-2H3 Cell Activation
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초록
Fagopyrum esculentum(FE) is an important food crop and medicinal plant that is used to improve diabetes, obesity, hypertension, hypercholesterolemia and constipation in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We investigated the effects on the release of inflammatory mediator and proximal signal events in FcεRI-mediated RBL-2H3 cell activation. FE reduced antigen (DNP–HSA)-induced release of histamine, prostaglandin D2 (PGD2) and cysteinyl Leukotriene (cysLT) in IgE-sensitized RBL-2H3 cells. In addition, it inhibited antigen-induced HDC2 and COX-2 and 5-LO mRNA expression in IgE-sensitized RBL-2H3 cells. FE also suppressed antigen-induced FcεRIβ and FcεRIγ subunit mRNA expression in these cells. To identify the mechanisms underpinning the inhibition of release of inflammatory mediators such as histamine and PGD2 and cysLT by FE, we examined the proximal signal events of intracellular FceRI signaling molecules. FE suppressed antigen-induced phosphorylation of Lyn, Syk, LAT, PLCγ1, PI3K, Akt and cPLA2. Collectively, the anti-allergic effects of FE in vitro suggest its possible therapeutic application to inflammatory allergic diseases, in which its inhibition of inflammatory mediator and FceRI-dependent signaling events in mast cells may be hugely beneficial.
Abstract
Fagopyrum esculentum(FE) is an important food crop and medicinal plant that is used to improve diabetes, obesity, hypertension, hypercholesterolemia and constipation in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We investigated the effects on the release of inflammatory mediator and proximal signal events in FcεRI-mediated RBL-2H3 cell activation. FE reduced antigen (DNP–HSA)-induced release of histamine, prostaglandin D2 (PGD2) and cysteinyl Leukotriene (cysLT) in IgE-sensitized RBL-2H3 cells. In addition, it inhibited antigen-induced HDC2 and COX-2 and 5-LO mRNA expression in IgE-sensitized RBL-2H3 cells. FE also suppressed antigen-induced FcεRIβ and FcεRIγ subunit mRNA expression in these cells. To identify the mechanisms underpinning the inhibition of release of inflammatory mediators such as histamine and PGD2 and cysLT by FE, we examined the proximal signal events of intracellular FceRI signaling molecules. FE suppressed antigen-induced phosphorylation of Lyn, Syk, LAT, PLCγ1, PI3K, Akt and cPLA2. Collectively, the anti-allergic effects of FE in vitro suggest its possible therapeutic application to inflammatory allergic diseases, in which its inhibition of inflammatory mediator and FceRI-dependent signaling events in mast cells may be hugely beneficial.
참고문헌 (24)
- Metcalfe, D. D., "Mast cells", Physiol Rev, 77(4), pp.1033~1079 (1997)
- Gilfillan, A. M., "The tyrosine kinase network regulating mast cell activation", Immunol Rev, 228(1), pp.149~169 (2009)
- 허준, "(신대역) 동의보감", pp.1808~ (2007), 법인문화사
- 중약대사전 편찬위원회, "중약대사전", pp.392~393 (2006), 정담
- Hur, S. J., "Effect of buckwheat extract on the antioxidant activity of lipid in mouse brain and its structural change during in vitro human digestion", J. Agric. Food Chem, 59(19), pp.10699~10704 (2011)
- van den Berg, A. J. J., "An in vitro examination of the antioxidant and anti-inflammatory properties of buckwheat honey", J Wound Care, 17(4), pp.172~178 (2008)
- Ishii, S., "Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice", Bioscience, Biotechnology, and Biochemistry, 72(12), pp.3148~3157 (2008)
- Li, S. Q., "Advances in the development of functional foods from buckwheat", Crit Rev Food Sci Nutr, 41(6), pp.451~464 (2001)
- Lee, C.C, "Fagopyrum tataricum (Buckwheat) Improved High-Glucose-Induced Insulin Resistance in Mouse Hepatocytes and Diabetes in Fructose-Rich Diet-Induced Mice", Exp Diabetes Res (2012)
- 노성택, "교맥 에탄올 추출물의 피부 미백기전 연구", 동의생리병리학회지, 21(5), pp.1243~1249 (2007), 대한동의병리학회
- Fukui, H, "Progress in Allergy Signal Research on Mast Cells: Up-Regulation of Histamine Signal-Related Gene Expression in Allergy Model Rats", Journal of Pharmacological Sciences, 106(3), pp.325~331 (2008)
- Murphy, R. C., "Biosynthesis and metabolism of leukotrienes", Biochemical Journal, 405(3), pp.379~ (2007)
- Boyce, J. A, "Mast cells and eicosanoid mediators: a system of reciprocal paracrine and autocrine regulation", Immunological Reviews, 217(1), pp.168~185 (2007)
- Tanaka, K., "Cutting Edge: Differential Production of Prostaglandin D2 by Human Helper T Cell Subsets", J Immunol, 164(5), pp.2277~2280 (2000)
- Westcott, J. Y., "Arachidonate-induced eicosanoid synthesis in RBL-2H3 cells: stimulation with antigen or A23187 induces prolonged activation of 5-lipoxygenase", Biochim. Biophys. Acta, 1303(1), pp.74~81 (1996)
- Blank, U., "Complete structure and expression in transfected cells of high affinity IgE receptor", Nature, 337(6203), pp.187~189 (1989)
- Ra, C., "Complete structure of the mouse mast cell receptor for IgE (Fc epsilon RI) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells", J. Biol. Chem, 264(26), pp.15323~15327 (1989)
- Ravetch, J. V., "Fc Receptors", Annual Review of Immunology, 9(1), pp.457~492 (1991)
- Blank, U., "Characterization of truncated alpha chain products from human, rat, and mouse high affinity receptor for immunoglobulin E", J. Biol. Chem, 266(4), pp.2639~2646 (1991)
- Lin, S., "The FcεRIβ Subunit Functions as an Amplifier of FcεRIγ-Mediated Cell Activation Signals", Cell, 85(7), pp.985~995 (1996)
- Kinet, J. P., "The high-affinity IgE receptor (Fc epsilon RI): from physiology to pathology", Annu. Rev. Immunol, 17, pp.931~972 (1999)
- Zhang, J., "Phosphorylation of Syk Activation Loop Tyrosines Is Essential for Syk Function AN IN VIVO STUDY USING A SPECIFIC ANTI-Syk ACTIVATION LOOP PHOSPHOTYROSINE ANTIBODY", J. Biol. Chem, 275(45), pp.35442~35447 (2000)
- Kalesnikoff, J., "New developments in mast cell biology", Nat Immunol, 9(11), pp.1215~1223 (2008)
- Fukao, T., "Role of phosphoinositide 3-kinase signaling in mast cells: new insights from knockout mouse studies", J. Mol. Med, 81(9), pp.524~535 (2003)