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희첨 추출물이 B16F10 세포에서 Melanin 생성과 Tyrosinase 활성에 미치는 억제효과

Inhibitory Effects of Siegesbeckia Herba Extracts on the Melanin Production and Tyrosinase Activity in B16F10 cells

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Objective : Siegesbeckia Herba can treat various skin disease by expelling wind and removing dampness andclearing away heat and toxic material effects. This study was designed to investigate effects of Siegesbeckia HerbaExtracts(SHE) on skin elasticity and whitening using B16F10 cell lines. Method : In this experiment, We observed effect of SHE on cell viability, inhibition of melanin synthesis andinhibitory effect on tyrosinase and elastase. Results : 1. SHE treated group showed decreased cell viability rates significantly compared with non-treatedgroup. More than SHE 250 ㎍/㎖, 500 ㎍/㎖ and 1,000 ㎍/㎖ of treated groups were lower levels ofmelanin synthesis respectively. 2. SHE significantly showed tyrosinase inhibitory activity in vitro, SHE increased tyrosinase inhibitoryactivity and elastase inhibitory activity in B16F10 cells, and tyrosinase inhibitory activity in vitro. 3. Tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, tyrosinase inhibitoryactivity in vitro were not accepted statistical significance compared with non-treated group. 4. SHE treated group showed increased SOD-like activity rates significantly compared with non-treatedgroup. More than SHE 250 ㎍/㎖, 500 ㎍/㎖ and 1,000 ㎍/㎖ of treated groups were lower levels ofmelanin synthesis respectively. Conclusion : These results suggest that SHE can inhibit melanin synthesis and tyrosinase inhibtory activity. So,We suggest that SHE can be maintained skin whitening.

Abstract

Objective : Siegesbeckia Herba can treat various skin disease by expelling wind and removing dampness andclearing away heat and toxic material effects. This study was designed to investigate effects of Siegesbeckia HerbaExtracts(SHE) on skin elasticity and whitening using B16F10 cell lines. Method : In this experiment, We observed effect of SHE on cell viability, inhibition of melanin synthesis andinhibitory effect on tyrosinase and elastase. Results : 1. SHE treated group showed decreased cell viability rates significantly compared with non-treatedgroup. More than SHE 250 ㎍/㎖, 500 ㎍/㎖ and 1,000 ㎍/㎖ of treated groups were lower levels ofmelanin synthesis respectively. 2. SHE significantly showed tyrosinase inhibitory activity in vitro, SHE increased tyrosinase inhibitoryactivity and elastase inhibitory activity in B16F10 cells, and tyrosinase inhibitory activity in vitro. 3. Tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, tyrosinase inhibitoryactivity in vitro were not accepted statistical significance compared with non-treated group. 4. SHE treated group showed increased SOD-like activity rates significantly compared with non-treatedgroup. More than SHE 250 ㎍/㎖, 500 ㎍/㎖ and 1,000 ㎍/㎖ of treated groups were lower levels ofmelanin synthesis respectively. Conclusion : These results suggest that SHE can inhibit melanin synthesis and tyrosinase inhibtory activity. So,We suggest that SHE can be maintained skin whitening.

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