펄스형 전자기장(PEMF) 자극을 이용한 DNCB로 유발된 아토피성 피부염 개선 효과
Effect on Improvement of Atopic Dermatitis Induced by DNCB using PEMF(Pulsed Electromagnetic Fields) Stimulation
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Objective: The purpose of this study is to evaluate the improvement of atopic dermatitis induced by DNCB using pulsed electromagnetic fields (PEMF) stimulation through animal models. Methods: Twenty 6-week Hairless Mouse (SKH1) models were randomly divided into normal groups (n=5), PEMF 15 Hz groups (n=5), PEMF 75 Hz groups (n=5), and Sham groups (n=5). To induce uniform atopic dermatitis, the DNCB(2,4-Dinitrochlorobenzene) solution was applied for 6 weeks. During the process of skin inflammation, sensory evaluations were conducted every 3~4 days to grade symptoms of atopic dermatitis. Following the onset of atopic dermatitis symptoms, the PEMF stimulation (15 mT) in the PEMF group (15 Hz & / 75 Hz) was stimulated for 8 hours per day for 1 week. After all experiments, the dosal skin tissue was sectioned at 4 μm, and after H&E Staining, the skin tissue was observed through the light microscopy to analyze the skin lesion size. Results: As a result of the sensory evaluation, the difference in average sensory evaluation scores between PEMF groups (15 Hz & 75 Hz) was 0.4 points, indicating a low statistical significance. Between the PEMF 15 Hz Group and the Sham Group, the difference in average sensory evaluation scores was reduced to 1.6 points, resulting in high statistical significance (P<0.05) and the best effect. As a result of analyzing the size of the lesion, the difference in size of the lesion area between PEMF groups (15 Hz & 75 Hz) was 807 pixels, indicating a low statistical significance. Between the PEMF group (15 Hz & 75 Hz) and the Sham group, the size difference of the lesion area was 12946 pixels, 13753 pixels, respectively, indicating high statistical significance (P<0.001). It means that inflammation has improved in dorsal skin tissue. Conclusions: This study confirms that PEMF (15 Hz / 75 Hz, 15 mT) stimulation can improve symptoms of DNCB-induced atopic dermatitis.
Abstract
Objective: The purpose of this study is to evaluate the improvement of atopic dermatitis induced by DNCB using pulsed electromagnetic fields (PEMF) stimulation through animal models. Methods: Twenty 6-week Hairless Mouse (SKH1) models were randomly divided into normal groups (n=5), PEMF 15 Hz groups (n=5), PEMF 75 Hz groups (n=5), and Sham groups (n=5). To induce uniform atopic dermatitis, the DNCB(2,4-Dinitrochlorobenzene) solution was applied for 6 weeks. During the process of skin inflammation, sensory evaluations were conducted every 3~4 days to grade symptoms of atopic dermatitis. Following the onset of atopic dermatitis symptoms, the PEMF stimulation (15 mT) in the PEMF group (15 Hz & / 75 Hz) was stimulated for 8 hours per day for 1 week. After all experiments, the dosal skin tissue was sectioned at 4 μm, and after H&E Staining, the skin tissue was observed through the light microscopy to analyze the skin lesion size. Results: As a result of the sensory evaluation, the difference in average sensory evaluation scores between PEMF groups (15 Hz & 75 Hz) was 0.4 points, indicating a low statistical significance. Between the PEMF 15 Hz Group and the Sham Group, the difference in average sensory evaluation scores was reduced to 1.6 points, resulting in high statistical significance (P<0.05) and the best effect. As a result of analyzing the size of the lesion, the difference in size of the lesion area between PEMF groups (15 Hz & 75 Hz) was 807 pixels, indicating a low statistical significance. Between the PEMF group (15 Hz & 75 Hz) and the Sham group, the size difference of the lesion area was 12946 pixels, 13753 pixels, respectively, indicating high statistical significance (P<0.001). It means that inflammation has improved in dorsal skin tissue. Conclusions: This study confirms that PEMF (15 Hz / 75 Hz, 15 mT) stimulation can improve symptoms of DNCB-induced atopic dermatitis.
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