NC/Nga 마우스에서의 아토피피부염 유사 병변에 대한 Light Emitting Diode의 치료 효과
Therapeutic Effects of Light Emitting Diode on Atopic Dermatitis-Like Lesions in NC/Nga Mice
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease. It is caused by immunological abnormalities,abnormalities of the skin barrier, and environmental/genetic factors. We did a preclinical trial to identify the effects of the 633-nm light- emitting diode (LED) and 830-nm LED for AD-like lesions in NC/Nga mice. Methods: AD-like skin lesions were induced by topical application of Dermatophagoides farinae extract on the skin of 5-week-old NC/Nga mice for 2 weeks, and then was treated with 630-nm or 830-nm LED for 1 week. We identified any therapeutic effects on AD using modified SCORAD index, skin biopsy, and measurements of both transepidermal water loss (TEWL) and proinflammatory cytokines. Results: Both of 630-nm and 830-nm LED treatment groups showed significantly reduced SCORAD indices and TEWLs at the end of treatment, compared to the non-treatment group. In addition, the levels of proinflammatory cytokine levels in both of the LED treatment groups were significantly decreased compared to those in the non-treatment group. Conclusion: These results show that 633-nm and 830-nm LED treatments can improve -like lesions in NC/Nga mice.
Abstract
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease. It is caused by immunological abnormalities,abnormalities of the skin barrier, and environmental/genetic factors. We did a preclinical trial to identify the effects of the 633-nm light- emitting diode (LED) and 830-nm LED for AD-like lesions in NC/Nga mice. Methods: AD-like skin lesions were induced by topical application of Dermatophagoides farinae extract on the skin of 5-week-old NC/Nga mice for 2 weeks, and then was treated with 630-nm or 830-nm LED for 1 week. We identified any therapeutic effects on AD using modified SCORAD index, skin biopsy, and measurements of both transepidermal water loss (TEWL) and proinflammatory cytokines. Results: Both of 630-nm and 830-nm LED treatment groups showed significantly reduced SCORAD indices and TEWLs at the end of treatment, compared to the non-treatment group. In addition, the levels of proinflammatory cytokine levels in both of the LED treatment groups were significantly decreased compared to those in the non-treatment group. Conclusion: These results show that 633-nm and 830-nm LED treatments can improve -like lesions in NC/Nga mice.
참고문헌 (21)
- 황선욱, "아토피피부염 환자의 치료경과에 따른 중증도 평가에서 SCORAD Index와 기계적 측정값과의 상관관계", 대한피부과학회지, 48(4), pp.266~271 (2010), 대한피부과학회
- 임정우, "아토피피부염 환자의 발병과 치료에 대한 실태 조사", 대한소아알레르기및호흡기학회지, 15(1), pp.44~52 (2005), 대한 소아알레르기 호흡기학회
- 김정희, "아토피피부염의 최신지견", 대한소아알레르기및호흡기학회지, 14(1), pp.12~23 (2004), 대한 소아알레르기 호흡기학회
- 주영현, "NC", Nga 마우스에서의 아토피피부염 모델 개발 및 피부염 병변에 대한 다래 추출물(Actinidia Extract)의 효과, 2009, pp.47(10), 1105~1112[DOI]
- Barolet D, "Light-emitting diodes (LEDs) in dermatology", Semin Cutan Med Surg, 27, pp.227~238 (2008)
- Minami Y, "Peroxidized cholesterol-induced matrix metalloproteinase- 9 activation and its suppression by dietary beta-carotene in photoaging of hairless mouse skin", J Nutr Biochem, 20, pp.389~398 (2009)
- 김지영, "NC", Nga 마우스에서의 아토피피부염 병변에 대한 신나무 잎 추출물의 효과, 2010, pp.48(11), 913~918[DOI]
- Kochevar IE, "Fundamentals of cutaneous photobiology and photoimmunology, In Fizpatrick’s dermatology in general medicine. 7th ed", pp.797~809 (2008), Mc Graw Hill Medical
- Byun HJ, "Full-spectrum light phototherapy for atopic dermatitis", Int J Dermatol, 50, pp.94~101 (2011)
- Levy ML, "Atopic dermatitis: understanding the disease and its management", Curr Med Res Opin, 23, pp.3091~3103 (2007)
- Brenninkmeijer EE, "Excimer laser vs. clobetasol propionate 0·05% ointment in prurigo form of atopic dermatitis: a randomized controlled trial, a pilot", Br J Dermatol, 163, pp.823~831 (2010)
- Morita H, "Clinical application of low reactive level laser therapy (LLLT) for atopic dermatitis", Keio J Med, 42, pp.174~176 (1993)
- Yang CH, "Photodynamic therapy for bowenoid papulosis using a novel incoherent light-emitting diode device", Br J Dermatol, 149, pp.1297~1299 (2003)
- 박미경, "LED 광 치료기의 최적 광 출력거리 측정", 대한피부과학회지, 49(02), pp.125~130 (2011), 대한피부과학회
- Ablon G, "Combination 830-nm and 633-nm light-emitting diode phototherapy shows promise in the treatment of recalcitrant psoriasis: preliminary findings", Photomed Laser Surg, 28, pp.141~146 (2010)
- Trelles MA, "Combined visible light and infrared light-emitting diode (LED) therapy enhances wound healing after laser ablative resurfacing of photodamaged facial skin", Med Laser Appl, 21, pp.165~175 (2006)
- Whelan HT, "Effect of NASA light-emitting diode irradiation on wound healing", J Clin Laser Med Surg, 19, pp.305~314 (2001)
- Lim W, "The anti-inflammatory mechanism of 635 nm light-emitting-diode irradiation compared with existing COX inhibitors", Lasers Surg Med, 39, pp.614~621 (2007)
- Nomura K, "Inhibition of inter leukin-1beta production and gene expression in human gingival fibroblasts by low-energy laser irradiation", Lasers Med Sci, 16, pp.218~223 (2001)
- Galderhead RG, "One mechanism behind LED phototherapy for wound healing and skin rejuvenation: key role of the mast cell", Laser Therapy, 17, pp.141~148 (2008)
- Graevskaya EE, "Effect of middle-wave ultraviolet irradiation and red light on degranulation of peritoneal mast cell in rats", Bull Exp Biol Med, 129, pp.357~358 (2000)