Real - Q RV Detection Kit를 이용한 호흡기 감염을 유발하는 바이러스의 동정 성능 평가
Evaluation of the Real-Q RV Detection Kit for the Identification of Viruses That Result in Respiratory Infections
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참고문헌
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초록
호흡기바이러스 감염은 세계적으로 가장 흔한 감염성 질환이며 사망을 유발하는 주요 질환 중 하나로 신속하고 정확한 원인바이러스의 검출이 중요하다. 이번 연구에서 실시간 다중 PCR kit 인 Real-Q RV Detection kit(BioSewoom, Korea) 의 호흡기 바이러스 검출한계와 임상적 유용성을 평가하고자 하였다. 총 10 주의 표준 바이러스를 대상으로 하여 Real-Q RV Detection kit 의 검출 한계를 측정하였으며, Allplex Respiratory Panel Assay kit (Seegene, Korea) 와의 비교를 위해 123 개의 비인후 도찰물, 객담 등의 호흡기 검체를 대상으로 하여 제조사의 권고방법대로 검사하였다. 두 kit 간 불일치 결과를 보이는 검체는 염기서열 분석을 시행하였다. 표준바이러스 중 Human rhinovirus (HRV) 의 검출한계가 가장 낮았으며, Parainfuenza virus 2 (PIV 2) 와 Parainfuenza virus 3 (PIV 3)는 상대적으로 높은 검출한계를 보였다. 두 kit 간 일치율은 95.9%에서 100% 까지 나타났다. Real-Q RV Detection kit 에서 Adenovirus (AdV), Allplex Respiratory Panel kit 에서 음성을 보인 검체(3건)는 염기서열분석 결과 모두 AdV 가 확인되었고, Real-Q RV Detection kit 에서 HRV, Allplex Respiratory Panel kit 에서 음성을 보인 검체(1 건)는 염기서열분석 결과 HRV 가 확인되었다. Real-Q RV Detection kit 에서 HRV 로 양성 , Allplex Respiratory Panel kit 에서 Human enterovirus (HEV)가 검출된 검체(1건)는 염기서열분석 결과 HRV 가 확인되었다. Real-Q RV Detection kit 는 호흡기 바이러스의 검출에 있어서 유용할 것이다.
Abstract
Viral respiratory infections are one of the most common infections worldwide. It is important to detect the virus early and precisely. In this study, we evaluated the limit of detection (LoD) and usefulness of the Real-Q RV Detection kit (BioSewoom, Seoul, Korea). We measured the LoD of the Real-Q RV Detection kit using 10 strains of standard viruses. We then compared the detection results by the Allplex Respiratory Panel Assay kit (Seegene, Seoul, Korea) using 123 clinical specimens. The discrepant results were confirmed by sequencing. Among the 10 standard viruses, the LoD of human rhinovirus (HRV) was the lowest and that of parainfluenza virus 2 and 3 was relatively high as detected by Real-Q RV Detection kit. Agreements of the two kits ranged from 95.9% to 100%. Three specimens detected negative by the Allplex Respiratory Panel kit were detected as adenovirus (AdV) by the Real-Q RV Detection kit and were confirmed by sequencing. Similarly, a specimen detected negative by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. A specimen detected as human enterovirus by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. Real-Q RV Detection kit showed good diagnostic performance and can be useful for detecting major viruses that cause respiratory infections.
참고문헌 (16)
- Thompson WW, "Mortality associated with influenza and respiratory syncytial virus in the United States", JAMA, 289, pp.179~186 (2003)
- Lee KH, "The role of respiratory viruses in the etiology of bacterial pneumonia : an ecological perspective", Evol Med Public Health, pp.95~109 (2016)
- Garbino J, "Lower respiratory viral illnesses : improved diagnosis by molecular methods and clinical impact", Am J Respir Crit Care Med, 170, pp.1197~1203 (2004)
- Charles PG, "Early diagnosis of lower respiratory tract infections(point-of-care tests)", Curr Opin Pulm Med, 14, pp.176~182 (2008)
- Vallières E, "Clinical and economical impact of multiplex respiratory virus assays", Diag Microbiol Infect Dis, 76, pp.255~261 (2013)
- Mahony JB, "Molecular diagnosis of respiratory virus infections", Crit Rev Clin Lab Sci, 48, pp.217~249 (2011)
- 김솔잎, "사람호흡기바이러스검출에 대한 다중PCR과 단일PCR의 성능 비교", Laboratory Medicine Online, 6(4), pp.240~245 (2016), 대한진단검사의학회
- 임인수, "Evaluation of a Multiplex Real-time PCR Assay for the Detection of Respiratory Viruses in Clinical Specimens", Annals of Laboratory Medicine, 32(6), pp.399~406 (2012), 대한진단검사의학회
- Huh HJ, "Performance evaluation of Allplex Respiratory Panels 1, 2, and 3 for detection of respiratory viruses and influenza A virus subtypes", J Clin Microbiol, 55, pp.479~484 (2017)
- Butt S, "Comparison of three commercial RT-PCR systems for the detection of respiratory viruses", J Clin Virol, 61, pp.406~410 (2014)
- Krunic N, "xTAG RVP assay : analytical and clinical performance", J Clin Virol, 40, pp.S39~S46 (2007)
- Gharabaghi F, "Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children", Clin Microbiol Infect, 17, pp.1900~1906 (2011)
- Jo YA, "Interpretation of weak-positive bands in a multiplex PCR using Seeplex RV12 ACE detection kit", J Lab Med Qual Assur, 33, pp.89~93 (2011)
- Le SY, "Conserved tertiary structure elements in the 5′ untranslated region of human enteroviruses and rhinoviruses", Virology, 191, pp.858~866 (1992)
- Dabisch-Ruthe M, "Comparison of three multiplex PCR assays for the detection of respiratory viral infections : evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay", BMC Infect Dis, 12, pp.163~ (2012)
- 고대현, "Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses", Annals of Laboratory Medicine, 37(5), pp.408~414 (2017), 대한진단검사의학회