Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Background: Respiratory tract infections are majorpublic health threats, and the identification of theircausative microbes helps clinicians to initiate timelyand appropriate antimicrobial therapy and prevent thesecondary spread of infection. The main goal of thisstudy was to compare two multiplex real-time polymerasechain reaction (PCR) assays used to detectrespiratory viral pathogens in nasopharyngeal swabspecimens. Methods: Between September and October 2017, atotal of 84 nasopharyngeal specimens were obtainedconsecutively from patients in a tertiary hospital usinga flocked swab with 3 mL universal transport medium(COPAN Diagnostics, USA). A total of 64 positiveand 20 negative sample results from the LGAdvanSure RV real-time RT-PCR kit (LG LifeSciences, Korea) were further retested using a newAdvanSure RV-plus a real-time RT-PCR kit to comparetheir performance. Results: Statistical analysis of positive and negativeagreement between the two different kits was conductedbetween the newly introduced AdvanSureRV-plus real-time RT-PCR kit and the AdvanSure RVreal-time RT-PCR. The overall agreement was 96.4%,with positive agreement of 98.4% and negativeagreement of 90%. The evaluated sensitivity andspecificity of AdvanSure RV-plus real-time RT-PCRwere 96.9% and 94.7%, respectively, with a kappavalue of 0.9 (P＜0.001). Conclusion: The performances of LG AdvanSure RVreal-time RT-PCR and the new AdvanSure RV-plusreal-time RT-PCR kit showed strong overall agreement. AdvanSure RV-plus real-time RT-PCR had a betterdetection rate and could detect coronavirus 229Eand enterovirus, especially with a high detection ratein coinfection. AdvanSure RV-plus real-time RT-PCRcan be considered a useful tool for respiratory virusdiagnosis in clinical laboratories.
Abstract
Background: Respiratory tract infections are majorpublic health threats, and the identification of theircausative microbes helps clinicians to initiate timelyand appropriate antimicrobial therapy and prevent thesecondary spread of infection. The main goal of thisstudy was to compare two multiplex real-time polymerasechain reaction (PCR) assays used to detectrespiratory viral pathogens in nasopharyngeal swabspecimens. Methods: Between September and October 2017, atotal of 84 nasopharyngeal specimens were obtainedconsecutively from patients in a tertiary hospital usinga flocked swab with 3 mL universal transport medium(COPAN Diagnostics, USA). A total of 64 positiveand 20 negative sample results from the LGAdvanSure RV real-time RT-PCR kit (LG LifeSciences, Korea) were further retested using a newAdvanSure RV-plus a real-time RT-PCR kit to comparetheir performance. Results: Statistical analysis of positive and negativeagreement between the two different kits was conductedbetween the newly introduced AdvanSureRV-plus real-time RT-PCR kit and the AdvanSure RVreal-time RT-PCR. The overall agreement was 96.4%,with positive agreement of 98.4% and negativeagreement of 90%. The evaluated sensitivity andspecificity of AdvanSure RV-plus real-time RT-PCRwere 96.9% and 94.7%, respectively, with a kappavalue of 0.9 (P＜0.001). Conclusion: The performances of LG AdvanSure RVreal-time RT-PCR and the new AdvanSure RV-plusreal-time RT-PCR kit showed strong overall agreement. AdvanSure RV-plus real-time RT-PCR had a betterdetection rate and could detect coronavirus 229Eand enterovirus, especially with a high detection ratein coinfection. AdvanSure RV-plus real-time RT-PCRcan be considered a useful tool for respiratory virusdiagnosis in clinical laboratories.
참고문헌 (29)
- Moesker FM, "Viruses as sole causative agents of severe acute respiratory tract infections in children", PLoS One, 11, pp.e0150776~ (2016)
- Tregoning JS, "Respiratory viral infections in infants:causes, clinical symptoms, virology, and immunology", Clin Microbiol Rev, 23, pp.74~98 (2010)
- Legand A, "Addressing the public health burden of respiratory viruses : the Battle against Respiratory Viruses(BRaVe)initiative", Future Virol, 8, pp.953~968 (2013)
- Pinky L, "Coinfections of the respiratory tract: viral competition for resources", PLoS One, 11, pp.e0155589~ (2016)
- Mehta S, "Ambient particulate air pollution and acute lower respiratory infections : a systematic review and implications for estimating the global burden of disease", Air Qual Atmos Health, 6, pp.69~83 (2013)
- Salez N, "Evaluation of four commercial multiplex molecular tests for the diagnosis of acute respiratory infections", PLoS One, 10, pp.e0130378~ (2015)
- Peaper DR, "Rapid diagnosis of influenza : state of the art", Clin Lab Med, 34, pp.365~385 (2014)
- Hodinka RL, "Point-counterpoint: is the era of viral culture over in the clinical microbiology laboratory?", J Clin Microbiol, 51, pp.2~8 (2013)
- Basile K, "Point-of-care diagnostics for respiratory viral infections", Expert Rev Mol Diagn, 18, pp.75~83 (2018)
- Souf S., "Recent advances in diagnostic testing for viral infections", Biosci Horiz, 9, pp.hwz010~ (2016)
- Loeffelholz MJ, "Rapid diagnosis of viral infections", Lab Med, 33, pp.283~286 (2002)
- Gowin E, "Assessment of the usefulness of multiplex real-time PCR tests in the diagnostic and therapeutic process of pneumonia in hospitalized children: a single-center experience", Biomed Res Int, 2017, pp.8037963~ (2017)
- Huang HS, "Multiplex PCR system for the rapid diagnosis of respiratory virus infection : systematic review and meta-analysis", Clin Microbiol Infect, 24, pp.1055~1063 (2018)
- Kralik P, "A basic guide to real time PCR in microbial diagnostics: definitions, parameters, and everything", Front Microbiol, 8, pp.108~ (2017)
- Krause JC, "The role of multiplex PCR in respiratory tract infections in children", Dtsch Arztebl Int, 111, pp.639~645 (2014)
- To KK, "Additional molecular testing of saliva specimens improves the detection of respiratory viruses", Emerg Microbes Infect, 6, pp.e49~ (2017)
- Cho CH, "Evaluation of the AdvanSureTM real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses", Diagn Microbiol Infect Dis, 79, pp.14~18 (2014)
- Kuan CS, "Detection of respiratory viruses from ARTI patients by xTAG RVP Fast v2assay and conventional methods", Malays J Med Sci, 24, pp.33~43 (2017)
- Piltcher OB, "How to avoid the inappropriate use of antibiotics in upper respiratory tract infections? A position statement from an expert panel", Braz J Otorhinolaryngol, 84, pp.265~279 (2018)
- Marshall DJ, "Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory setting", J Clin Microbiol, 45, pp.3875~3882 (2007)
- Kim H, "Comparison of two multiplex PCR assays for the detection of respiratory viral infections", Clin Respir J, 8, pp.391~396 (2014)
- Bibby DF, "Comparative evaluation of the Seegene Seeplex RV15 and real-time PCR for respiratory virus detection", J Med Virol, 83, pp.1469~1475 (2011)
- Christaki E, "New technologies in predicting, preventing and controlling emerging infectious diseases", Virulence, 6, pp.558~565 (2015)
- Jung YJ, "Comparison of the AdvanSureTM real-time RT-PCR and Seeplex®RV12 ACE assay for the detection of respiratory viruses", J Virol Methods, 224, pp.42~46 (2015)
- Boivin G, "Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses", J Clin Microbiol, 42, pp.45~51 (2004)
- Seung Gyu Yun, "Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV", Journal of Clinical Laboratory Analysis, 32(2), pp.e22230~ (2018), Wiley[DOI]
- Huh HJ, "Performance evaluation of Allplex respiratory panels 1, 2, and 3for detection of respiratory viruses and influenza A virus subtypes", J Clin Microbiol, 55, pp.479~484 (2017)
- Yan L, "Crystal structure of the post-fusion core of the Human coronavirus 229E spike protein at 1.86 Å resolution", Acta Crystallogr D Struct Biol, 74, pp.841~851 (2018)
- Asner SA, "Clinical severity of rhinovirus", enterovirus compared to other respiratory viruses in children, 2014, pp.8, 436~442