본문 바로가기
KERD
논문KCI 등재

분류군별 외래생물 탐지를 위한 환경 DNA 메타바코딩 활용 가능성

Feasibility of Environmental DNA Metabarcoding for Invasive Species Detection According to Taxa

전정은|송영근|한승우|강유진|원수연
한국환경영향평가학회 (Korean Society Of Environmental Impact Assessment)|2023.04|환경영향평가 (Journal of Environmental Impact Assessment)|제32권 제2호|pp. 94-111

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

Metabarcoding외래종Next generation sequencingeDNA차세대 염기서열 분석Invasive speciesBiological monitoring메타바코딩생물 모니터링환경 DNA
주제분류환경공학

초록

효과적인 외래생물 관리 전략 수립을 위해서는 도입 및 확산 여부 평가를 위한 정기 모니터링이 요구된다. 환경 DNA (eDNA, environmental DNA) 메타바코딩은 높은 검출 민감도를 가지고 다수의 종을동시에 검출할 수 있어 외래생물의 출현 여부와 그 영향을 평가하는데 활발히 활용되고 있다. 국내에서는어류를 중심으로 메타바코딩의 적용 가능성 평가가 이루어지고 있으며 타 분류군에 대한 연구는 부족한 실정이다. 따라서 본 연구에서는 환경 DNA 메타바코딩을 활용한 국내 외래생물 탐지 가능성을 확인하고자했다. 분류군별 검출 가능성을 확인하기 위해 어류, 포유류, 조류, 양서류를 목표로 디자인 된 4가지 범용 프라이머(MiFish, MiMammal, Mibird, Amp16S)를 활용하여 대상종 검출 여부를 평가하였다. 그 결과, 총55개 지점 중 17개 지점(Trachemys scripta, 3개 지점; Cervus nippon, 3개 지점; Micropterus salmoides, 7개 지점; Rana catesbeiana, 4개 지점)에서 대상종의 서식이 확인되었다. 대상지 내 조밀한 지점 선정에도 생태적 특성을 반영한 검출 지점에 차이가 나타났다. 큰입배스와 붉은귀거북을 중심으로 외래생물이 출현이 생물 군집구조(종 풍부도, 풍부도, 다양도)에 미치는 영향을 비교한 결과, 외래생물이 서식하는 지점에서의 다양도가 더 높게 나타났다. 또한 외래생물 출현 지점에서 출현 종 수가 1~4종 추가 검출되었으며풍부도 또한 1.7배 높게 나타났다. 메타바코딩을 통한 외래생물 검출 결과 및 군집구조 비교는 eDNA를 통한 다량의 모니터링 데이터 구축이 다차원적 생태계 평가에 효율적으로 활용될 수 있음을 나타냈다. 또한환경의 인위적, 자연적 변화에 따른 생물상 변화를 관찰하고 자연생태 분야의 환경영향평가 등 현황 평가및 예측을 위한 주요한 기초자료로 활용 가능성을 제시하였다.

Abstract

In orderto establish an effective management strategy forinvasive species early detection and regular monitoring are required to assess their introduction or dispersal. Environmental DNA (eDNA) is actively applied to evaluate the fauna including the presence of invasive species as it has high detection sensitivity and can detect multiple species simultaneously. In Korea, the applicability evaluation of metabarcoding is being conducted mainly on fish, and research on other taxa is insufficient. Therefore, this study identified the feasibility of detecting invasive species in Korea using eDNA metabarcoding. In addition, to confirm the possibility of detection by taxa, the detection of target species was evaluated using four universal primers (MiFish, MiMammal, Mibird, Amp16S) designed for fish, mammals, birds, and amphibians. As a result, target species (Trachemys scripta, 3 sites; Cervus nippon, 3 sites; Micropterus salmoides, 7 sites; Rana catesbeiana, 4 sites) were detected in 17 of the total 55 sites. Even in the selection of dense sampling sites within the study area, there was a difference in the detection result by reflecting the ecological characteristics of the target species. A comparison of community structures (species richness, abundance and diversity) based on the presence of invasive species focused on M.salmoides and T.scripta, showed higher diversity at the point where invasive species were detected. Also, 1 to 4 more species were detected and abundance was also up to 1.7 times higher. The results of invasive species detection through metabarcoding and the comparison of community structures indicate that the accumulation of large amounts of monitoring data through eDNA can be efficiently utilized for multidimensional ecosystem evaluation. In addition, it suggested that eDNA can be used as major data for evaluation and prediction, such as tracking biological changes caused by artificial and natural factors and environmental impact assessment.

참고문헌 (47)

  1. Czeglédi I, "Congruency between two traditional and eDNA-based sampling methods in characterising taxonomic and trait-based structure of fish communities and community-environment relationships in lentic environment", Ecol Indic, 129, pp.107952~ (2021)
  2. Deiner K, "Transport Distance of Invertebrate Environmental DNA in a Natural River", PLoS One, 9, pp.e88786~ (2014)
  3. Robert M. Dorazio, "ednaoccupancy:An r package for multiscale occupancy modelling of environmental DNA data", Molecular Ecology Resources, 18(2), pp.368~380 (2017), Wiley[DOI]
  4. A Study on the Management and Utilization of the Saetgang Stream in the Han River, "Gang HS", Korea Environment Institute, ~ (2015)
  5. Hashemzadeh Segherloo I, "eDNA metabarcoding as a means to assess distribution of subterranean fish communities:Iranian blind cave fishes as a case study", Environmental DNA, 4(2), pp.402~416 (2022)[DOI]
  6. "Information of Korean Alien Specie"[URL]
  7. Rourke ML, "Environmental DNA(eDNA)as a tool for assessing fish biomass : A review of approaches and future considerations for resource surveys", Environmental DNA, 4(1), pp.9~33 (2022)[DOI]
  8. Aozora Kakuda, "Environmental DNA detection and quantification of invasive red-eared sliders, Trachemy scripta elegans, in ponds and the influence of water quality", PeerJ, 7, pp.e8155~ (2019), PeerJ[DOI]
  9. 김가우, "환경DNA 기술을 이용한 국내 담수어류종 탐지 가능성 - 경기도 민물고기생태학습관 중심으로 -", 환경영향평가, 30(1), pp.1~12 (2021), 한국환경영향평가학회
  10. 김정희, "환경유전자의 국내 담수어류 모니터링 적용 연구 김정희", 생태와 환경, 53(1), pp.63~72 (2020), 한국수생태학회[DOI]
  11. Leempoel K, "A comparison of eDNA to camera trapping for assessment of terrestrial mammal diversity", Proceedings of the Royal Society B, 287(1918) (2020)
  12. Feilong Li, "Application of Environmental DNA Metabarcoding for Predicting Anthropogenic Pollution in Rivers", Environmental Science & Technology (2018), American Chemical Society (ACS)[DOI]
  13. Lintermans M, "Finding the needle in the haystack : Comparing sampling methods for detecting an endangered freshwater fish", Marine and Freshwater Research, 67(11), pp.1740~1749 (2016)[DOI]
  14. Juan Diego Lozano Mojica, "Applications of eDNA Metabarcoding for Vertebrate Diversity Studies in Northern Colombian Water Bodies", Frontiers in Ecology and Evolution, 8 (2021), Frontiers Media SA[DOI]
  15. Toshifumi Minamoto, "Surveillance of fish species composition using environmental DNA", Limnology, 13(2), pp.193~197 (2011), Springer Science and Business Media LLC[DOI]
  16. "University"[URL]
  17. "Act On the Conservation And Use of Biological Diversity" (2022)[URL]
  18. "4th National Natural Environment Survey: Ichthyofauna from midstream catchment of Anyang stream" (2017)[URL]
  19. "4th National Natural Environment Survey: Ichthyofauna from midstream catchment of Anyang stream" (2017)[URL]
  20. "The 2nd Alien Speices Management Plan (2019-2023)" (2019)[URL]
  21. Developing a real-time web-based positioning surveillance system customized for introduced exotic species, "Ministry of Environment", Seoul National, ~ (2022)
  22. Mizumoto H, "Utilizing environmental DNA for wide-range distributions of reproductive area of an invasive terrestrial toad in Ishikari river basin in Japan", Biol Invasions, 24(4), pp.1199~1211 (2022)
  23. Miya M, "MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species", R Soc Open Sci, 2, pp.150088~ (2015)
  24. Muha TP, "Using environmental DNA to improve species distribution models for freshwater invaders", Front Ecol Evol, 5, pp.158~ (2017)
  25. Murakami H, "Dispersion and degradation of environmental DNA from caged fish in a marine environment", Fisheries Science, 85, pp.327~337 (2019)[DOI]
  26. Nevers MB, "Influence of sediment and stream transport on detecting a source of environmental DNA", PLoS One, 15, pp.e0244086~ (2020)
  27. Di MC, "Read counts from environmental DNA (eDNA) metabarcoding reflect fish abundance and biomass in drained ponds", Metabarcoding Metagenom, 4, pp.97~112 (2020)
  28. Rubenson ES, "An invader in salmonid rearing habitat: Current and future distributions of smallmouth bass (micropterus dolomieu) in the Columbia river basin", Canadian Journal of Fisheries and Aquatic Sciences, 77(2), pp.314~325 (2020)[DOI]
  29. Saenz-Agudelo P, "Monitoring vertebrate biodiversity of a protected coastal wetland using eDNA metabarcoding", Environmental DNA, 4(1), pp.77~92 (2022)[DOI]
  30. Sard NM, "Comparison of fish detections, community diversity, and relative abundance using environmental DNA metabarcoding and traditional gears", Environmental DNA, 1(4), pp.368~384 (2019)[DOI]
  31. Sato Y, "MitoFish and MiFish Pipeline: A Mitochondrial Genome Database of Fish with an Analysis Pipeline for Environmental DNA Metabarcoding", Mol Biol Evol, 35(6), pp.1553~1555 (2018)[DOI]
  32. Smart AS, "Environmental DNA sampling is more sensitive than a traditional survey technique for detecting an aquatic invader", Ecological Applications, 25(7), pp.1944~1952 (2015)[DOI]
  33. 송영근, "환경DNA 메타바코딩 기술을 활용한 수생태계 어류종 군집조사의 가능성 – 도시 생태하천 초기분석 자료를 중심으로", 한국환경복원기술학회지, 22(6), pp.125~138 (2019), 한국환경복원기술학회[DOI]
  34. Stewart KA, "Understanding the effects of biotic and abiotic factors on sources of aquatic environmental DNA", Biodiversity and Conservation, 28(5), pp.983~1001 (2019)[DOI]
  35. Stoeckle BC, "Influence of stream characteristics and population size on downstream transport of freshwater mollusk environmental dna", Freshwater Science, 40, pp.191~201 (2021)[DOI]
  36. Takahara T, "Estimation of Fish Biomass Using Environmental DNA", PLoS One, 7, pp.e35868~ (2012)
  37. Thomsen PF, "Monitoring endangered freshwater biodiversity using environmental DNA", Mol Ecol, 21(11), pp.2565~2573 (2012)[DOI]
  38. Thomsen PF, "Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity", Biol Conserv, 183, pp.4~18 (2015)
  39. Ushio M, "Environmental DNA enables detection of terrestrial mammals from forest pond water", Mol Ecol Resour, 17(6), pp.e63~e75 (2017)
  40. Ushio M, "Demonstration of the potential of environmental DNA as a tool for the detection of avian species", Sci Rep, 8(1), pp.1~10 (2018)
  41. Valentini A, "Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding", Mol Ecol, 25(4), pp.929~942 (2016)
  42. Vila M, "Handbook of Alien Species in Europe", pp.265~268 (2009)
  43. Williams KE, "Detection and persistence of environmental DNA from an invasive, terrestrial mammal", Ecol Evol, 8(1), pp.688~695 (2018)[DOI]
  44. Yamamoto S, "Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea", Scientific Reports, 7(1), pp.1~12 (2017)[DOI]
  45. Yates MC, "Meta-analysis supports further refinement of eDNA for monitoring aquatic species-specific abundance in nature", Environmental DNA, 1(1), pp.5~13 (2019)[DOI]
  46. A Study on the Basic Environment of Waterfront Ecological Culture for the Development of Waterfront Culture Revitalization Plan, "Yeoungdeungpo cultural foundation", ~ (2020)
  47. Yu Z, "Comparison of species-specific qPCR and metabarcoding methods to detect small pelagic fish distribution from open ocean environmental DNA", PLOS ONE, 17(9), pp.0273670~ (2022)

관련 논문AI추천

환경유전자 메타바코딩을 활용한 하천 어류 군집 분석곽민지, 김태현, 안희준, 오승은, 윤미라, 이강혁, 정주용, 조윤숙, 한송희, 홍순모한국환경분석학회 · 환경분석과 독성보건2023 · 제26권 제2호한강의 어류종 모니터링을 위한 환경 DNA 메타바코딩의 유용성 검토김창배, 문민호, 안형은한국환경분석학회 · 환경분석과 독성보건2024 · 제27권 제1호International Trends in the Application of Environmental DNA Methods to National Aquatic Ecosystem Monitoring Programs and Challenges for Domestic Implementation오혜지, 윤주덕, 장광현, 최예림국립생태원 · 국립생태원회보2025 · 제6권 제1호Modeling Species Distributions to Predict Seasonal Habitat Range of Invasive Fish in the Urban Stream via Environmental DNA강유진, 김영환, 송영근, 윤지원(Jiweon Yun), 신원협(Wonhyeop Shin)국립생태원 · 국립생태원회보2022 · 제3권 제1호환경DNA 기술을 이용한 국내 담수어류종 탐지 가능성 - 경기도 민물고기생태학습관 중심으로 -김가우, 송영근한국환경영향평가학회 · 환경영향평가2021 · 제30권 제1호환경 DNA 메타바코딩을 이용한 국립공원(설악산, 월악산, 덕유산, 한려해상) 하천생태계의 어류 군집구조 및 종 다양성고민섭, 김영래, 김유림, 김정은, 김희수, 서진규, 왕주현, 이혁제, 장지은, 최희규, 황순영한국환경생물학회 · 환경생물2025 · 제43권 제4호

이 간행물의 다른 논문 (7건)

메타개체군 이론을 활용한 도시개발에 따른 생물 종 영향 평가 활용 가능성 분석전윤호, 이동근, 최지영, 김은섭, 박태윤, 모용원pp. 61-71국립공원의 수질조절 생태계서비스 가치평가 연구 - 오대산국립공원을 중심으로 -김가우pp. 72-82전라남도 구례군에 서식하는 멸종위기 야생생물 II급 남생이의 서식지 적합성 모델 개발박창득, 유정우, 윤주덕, 권관익, 유나경, 허문성pp. 83-93토양세척 및 토양경작 정화 토양의 건강성 평가이용민, 성기준pp. 112-122배터리 제조공정에서 배출되는 잠재 유해 물질에 대한 물벼룩과 발광박테리아의 생태독성: 리튬, 니켈, 황산염을 대상으로성기준, 노인혜pp. 123-133
원문 보기