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International Trends in the Application of Environmental DNA Methods to National Aquatic Ecosystem Monitoring Programs and Challenges for Domestic Implementation

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To identify the spatiotemporal distribution of organisms by detecting DNA fragments released into the environment, environmental DNA (eDNA) is readily employed. This technique is receiving significant attention in ecological research, particularly in aquatic ecosystems, as an effective alternative method for national monitoring systems that need to simultaneously investigate diverse biological communities at multiple sites, at low costs and technical discrepancies. However, challenges remain, such as improving poor reliability of genetic information databases, standardizing application of methods, and interpretating of acquired genetic information, including issues of false positives and false negatives. This study explores international cases in which eDNA methods have been actively developed and applied, and summarizes the current state of research in South Korea and the various issues identified in these studies to guide individuals and domestic institutions regarding the application of eDNA technology as a targeted investigation or supplementary tool for future biological surveys.

Abstract

To identify the spatiotemporal distribution of organisms by detecting DNA fragments released into the environment, environmental DNA (eDNA) is readily employed. This technique is receiving significant attention in ecological research, particularly in aquatic ecosystems, as an effective alternative method for national monitoring systems that need to simultaneously investigate diverse biological communities at multiple sites, at low costs and technical discrepancies. However, challenges remain, such as improving poor reliability of genetic information databases, standardizing application of methods, and interpretating of acquired genetic information, including issues of false positives and false negatives. This study explores international cases in which eDNA methods have been actively developed and applied, and summarizes the current state of research in South Korea and the various issues identified in these studies to guide individuals and domestic institutions regarding the application of eDNA technology as a targeted investigation or supplementary tool for future biological surveys.

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