환경유전자 메타바코딩을 활용한 하천 어류 군집 분석
Using eDNA Metabarcoding for the Analysis of Fish Communities in Rivers
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The present study aims to assess the effectiveness of eDNA metabarcoding for the analysis of fish communities in rivers. The findings suggest that the methodology attained an accuracy rate of 80% for species identification and was able to detect between 79.2% and 87.5% of the fish species recorded in the fish monitoring database for three rivers of Anseong, Bokha and Gyeongan during the period 2019 to 2021. Significantly, the eDNA metabarcoding technique enabled the successful detection of comparatively larger fish species, such as Channa argus and Silurus asotus. Furthermore, depending on the river, this method identified 12 to 14 additional species that could not be observed through traditional methodologies. However, it is worth noting that the Mifish primer used amplifies short gene segments, which can pose challenges in identifying species with identical gene sequences. Notwithstanding this limitation, the advantages of eDNA analysis over conventional methods are significant, enabling the identification of a broader range of species within a shorter timeframe, using smaller sample volumes and minimizing risks to both endangered fish and to researchers. As a result, eDNA analysis represents a valuable alternative for assessing biodiversity and for collecting data on fish species that are challenging to analyze.
Abstract
The present study aims to assess the effectiveness of eDNA metabarcoding for the analysis of fish communities in rivers. The findings suggest that the methodology attained an accuracy rate of 80% for species identification and was able to detect between 79.2% and 87.5% of the fish species recorded in the fish monitoring database for three rivers of Anseong, Bokha and Gyeongan during the period 2019 to 2021. Significantly, the eDNA metabarcoding technique enabled the successful detection of comparatively larger fish species, such as Channa argus and Silurus asotus. Furthermore, depending on the river, this method identified 12 to 14 additional species that could not be observed through traditional methodologies. However, it is worth noting that the Mifish primer used amplifies short gene segments, which can pose challenges in identifying species with identical gene sequences. Notwithstanding this limitation, the advantages of eDNA analysis over conventional methods are significant, enabling the identification of a broader range of species within a shorter timeframe, using smaller sample volumes and minimizing risks to both endangered fish and to researchers. As a result, eDNA analysis represents a valuable alternative for assessing biodiversity and for collecting data on fish species that are challenging to analyze.
참고문헌 (27)
- P. Taberlet, "Environmental DNA", Molecular Ecology, 21, pp.1789~1793 (2012)
- P. F. Thomsen, "Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity", Biological Conservation, 183, pp.4~18 (2015)
- M. A. Barnes, "Environmental conditions influence eDNA persistence in aquatic systems", Environmental Science & Technology, 48, pp.1819~1827 (2014)
- K. Deiner, "Environmental DNA reveals that rivers are conveyer belts of biodiversity information", Nature Communication, 7, pp.12544~ (2016)
- A. Valentini, "Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding", Molecular Ecology, 25, pp.929~942 (2016)
- E. E. Sigsgaard, "Seawater environmental DNA reflects seasonality of a coastal fish community", Marine Biology, 164, pp.128~ (2017)
- E. -B. Kim, "Environmental DNA Metabarcoding Analysis of Fish Assemblages and Phytoplankton Communities in a Furrowed Seabed Area Caused by Aggregate Mining", Frontiers in Marine Science, 9 (2022)
- A. R. Kim, "Metabarcoding Analysis of Ichthyoplankton in the East", Japan Sea Using the Novel Fish-Specific Universal Primer Set, 2021, pp.8, ~
- 김가우, "환경DNA 기술을 이용한 국내 담수어류종 탐지 가능성 - 경기도 민물고기생태학습관 중심으로 -", 환경영향평가, 30(1), pp.1~12 (2021), 한국환경영향평가학회
- 송영근, "환경DNA 메타바코딩 기술을 활용한 수생태계 어류종 군집조사의 가능성 – 도시 생태하천 초기분석 자료를 중심으로", 한국환경복원기술학회지, 22(6), pp.125~138 (2019), 한국환경복원기술학회[DOI]
- 김정희, "환경유전자의 국내 담수어류 모니터링 적용 연구 김정희", 생태와 환경, 53(1), pp.63~72 (2020), 한국수생태학회[DOI]
- M. J. Alam, "Assessment of fish biodiversity in four Korean rivers using environmental DNA metabarcoding", PeerJ, 8, pp.e9508~ (2020)
- M. Miya, "MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes : detection of more than 230 subtropical marine species", Royal Society Open Science, 2, pp.150088~ (2015)
- M. Miya, "MiFish metabarcoding : a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples", Fisheries Science, 86, pp.939~970 (2020)
- Y. Sato, "MitoFish and MiFish Pipeline : A Mitochondrial Genome Database of Fish with an Analysis Pipeline for Environmental DNA Metabarcoding", Molecular Biology and Evolution, 35(6), pp.1553~1555 (2018)
- 김근식, "분석조건별 담수어류의 환경 DNA 메타바코딩 효율 비교: 필터, 추출 키트, 프라이머 조합 및 PCR 방법", 생태와 환경, 54(3), pp.199~208 (2021), 한국수생태학회[DOI]
- R. Hinlo, "Methods to maximise recovery of environmental DNA from water samples", PloS one, 12, pp.e0179251~ (2017)
- S. Tsuji, "The detection of aquatic macroorganisms using environmental DNA analysis—A review of methods for collection, extraction, and detection", Environmental DNA, 1, pp.99~108 (2019)
- L. Shu, "Standards for Methods Utilizing Environmental DNA for Detection of Fish Species", Genes, 11, pp.296~ (2020)
- H. -T. Ruan, "Effects of sampling strategies and DNA extraction methods on eDNA metabarcoding : A case study of estuarine fish diversity monitoring", Zoological Research, 43, pp.92~204 (2022)
- H. Itakura, "Environmental DNA analysis reveals the spatial distribution, abundance, and biomass of Japanese eels at the river-basin scale", Aquatic Conservation : Marine and Freshwater Ecosystems, 29, pp.361~373 (2019)
- M. L. Rourke, "Environmental DNA(eDNA)as a tool for assessing fish biomass : A review of approaches and future considerations for resource surveys", Environmental DNA, 4, pp.9~33 (2022)
- M. L. Rourke, "Poor utility of environmental DNA for estimating the biomass of a threatened freshwater teleost; but clear direction for future candidate assessments", Fisheries Research, 258, pp.106545~ (2023)
- R. P. Kelly, "Using Environmental DNA to Census Marine Fishes in a Large Mesocosm", PloS one, 9, pp.e86175~ (2014)
- T. Takahara, "Estimation of Fish Biomass Using Environmental DNA", PloS one, 7, pp.e35868~ (2012)
- L. e. Miranda, "Selecting Gear for Monitoring Fish Assemblages", Management and Ecology of River Fisheries, pp.3~13 (2000)
- G. Kumar, "Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary", PloS one, 17, pp.e0266720~ (2022)