멸종위기 수종 석곡(Dendrobium moniliforme)의 Protocorm-like Body 형성 및 기내증식
In vitro Propagation and Protocorm-like Body Formation of Endangered Species, Dendrobium moniliforme
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
This experiment was conducted to establish the in vitro propagation of Dendrobium moniliforme through protocorm like body (PLB) induction from the culture of leaf, stem and root explants. Frequency of PLB formation from root explants was higher than those of leaf and stem explants. PLB’s proliferation in MS medium including 1.0 mg/L NAA was better than 1.0 mg/L IBA. Frequency of PLB’s proliferation on medium with various concentrations of BA, Kinetin, Tidiazuron (0, 1.0, 3.0 mg/L) and NAA (0, 1.0 mg/L) was tested. The maximun proliferation of PLB’s was obtained on MS medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Addition of 200 mg/L activated charcoal (AC) and 30 g/L sucrose was effective on PLB proliferation for D. moniliforme.
Abstract
This experiment was conducted to establish the in vitro propagation of Dendrobium moniliforme through protocorm like body (PLB) induction from the culture of leaf, stem and root explants. Frequency of PLB formation from root explants was higher than those of leaf and stem explants. PLB’s proliferation in MS medium including 1.0 mg/L NAA was better than 1.0 mg/L IBA. Frequency of PLB’s proliferation on medium with various concentrations of BA, Kinetin, Tidiazuron (0, 1.0, 3.0 mg/L) and NAA (0, 1.0 mg/L) was tested. The maximun proliferation of PLB’s was obtained on MS medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Addition of 200 mg/L activated charcoal (AC) and 30 g/L sucrose was effective on PLB proliferation for D. moniliforme.
참고문헌 (36)
- Arditti J, "Micropropagation of orchids", pp.373~375 (1993), John Wiley and Sons
- Bae KH, "Factors affecting fruit baring in natural habitat and in vitro culture of zygotic embryos of Cypripedium japonicum", Prog Ornament Plant, 11, pp.146~152 (2013)
- Bae KH, "Structural changes of seed coats and stimulation of in vitro germination of fully mature seeds of Cypripedium macranthos Swartz (orchidaceae) by NaOCl pretreatment", Prog Ornament Plant, 10, pp.107~113 (2010)
- Bae KH, "In vitro germination and plantlet conversion from the culture of fully mature seeds of Cypripedium guttatum Swartz", Prog Ornament Plant, 9, pp.160~165 (2009)
- 배기화, "새우난초(Calanthe discolor)의 조직배양으로부터 다신초형성을 통한 토양순화", 한국자원식물학회지, 23(1), pp.7~13 (2010), 한국자원식물학회
- Chen YC, "A reliable protocol for plant regeneration from callus culture of Phalaenopsis", In Vitro Cell Dev Biol Plant, 36, pp.420~423 (2000)
- Chung JD, "Studies on aseptic culture of seeds in Dendrobium monile", J Korean Soc Hort Sci, 22, pp.139~145 (1981)
- Chung MY, "Effect of culture media on asymbiotic seed germination and those seedling growth of Calanthe discolor and Habenaria radiata", Korean J Plant Biotechnol, 25, pp.189~194 (1998)
- Chung JD, "Effect of medium composition on multiple shooting and subsequent growth of mericlone from rhizome of shoot tip cultures of temperate Cymbidium species", J Kor Soc Hort Sci, 39, pp.343~349 (2004)
- Goh CJ, "Micropropagation of the monopodial orchid hybrid Aranda ‘Deborah’ using inflorescence explants", Sci Hort, 44, pp.315~321 (1980)
- Hussey G, "In vitro release of axillary shoots from apical dominance in monocotyledonous plantlets", Ann Bot, 40, pp.1323~1325 (1976)
- Song WS, "Effect of plant growth regulators and medium salt strength on in vitro propagation of Belamcanda chinensis DC", Korean J Plant Res, 10, pp.114~121 (1997)
- Kim MS, "Micropropagation of Dendrobium hybrids through shoot tip culture", Acta Hort (ISHS), 624, pp.527~533 (2003)
- 김영균, "石斛의 항산화 효과", 대한본초학회지, 20(4), pp.53~60 (2005), 대한본초학회
- Koh JC, "Callus culture and plant Regeneration from shoot apex of Iris Sanguinea Donn ex. Horn", J Plant Biotechnol, 31, pp.19~23 (2005)
- Lee EJ, "Studies on in vitro cutting of Dendrobium moniliform native to Korea", J Kor Soc Hort Sci, 11, pp.314~315 (1993)
- Lee K, "The wild orchids of Korea", pp.193~ (1980), Jungsung publisher
- Madubanya LA, "Dierama luteoalbidum:Liquid culture provides an efficient system for the ex situ conservation of an endangered and horticulturally valuable plant", S Afr J Bot, 72, pp.584~588 (2006)
- Malabadi RB, "Efficient regeneration of Vanda coerulea, an endangered orchid using thidiazuron", Plant Cell Tiss Org Cult, 76, pp.289~293 (2004)
- Meyer HJ, "In vitro multiplication of Ixia fkexuosa", Hort Sci, 23, pp.1070~1071 (1998)
- Ministry of Environment (MEV), "Enforcement decrees drafted for the Wildlife Protection Act", 83~ (2005)
- Ministry of Environment (MEV), "Enforcement decrees drafted for the Wildlife Protection Act", 66~ (2012)
- 문흥규, "멸종위기 식물 덩굴용담의 기내번식에 미치는 생장조절제 효과", Journal of Plant Biotechnology, 36(1), pp.13~17 (2009), 한국식물생명공학회
- Murashige T, "A revised medium for rapid growth and bio assays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Nayak NR, "Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae)", Sci Hort, 94, pp.107~116 (2002)
- Park SY, "Protocorm-like body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis", Plant Sci, 164, pp.919~923 (2003)
- Park SY, "An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture", Plant Cell Rep, 21, pp.46~51 (2002)
- Raup DM, "Biological extinction in earth history", Science, 231, pp.1528~1533 (1986)
- Rotor Jr G, "A method of vegetative propagation of Phalaenopsis species and hybrids", Amer Orchid Soc Bull, 18, pp.738~739 (1950)
- Roy J, "Direct and callus- mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae)", Plant Cell Tiss Org Cult, 90, pp.31~39 (2007)
- Ryu JH, "Induction of haploid plants by anther culture in sesame (Sesaum indicum L.)", Kor J Plant Biotech, 19, pp.171~177 (1992)
- Schemske DW, "Evaluating approaches to the conservation of rare and endangered plants", Ecology, 75, pp.584~606 (1994)
- Shibli RA, "Somatic embryogenesis in the endemic Black Iris", Plant Cell Tiss Org Cult, 61, pp.15~21 (2000)
- Tanaka M, "High-tech and micropropagation IV" (1992), Springer
- the brink of extinction: conserving the diversity of life, "Wolfe SC", Worldwatch Institute, 53~, pp.World watch paper 78 (1987)
- 윤미영, "석곡MeOH 추출물이 H2O2에의한신경세포 보호효과에 미치는 영향", Applied Biological Chemistry, 50(1), pp.63~67 (2007), 한국응용생명화학회