팔손이 액아배양을 통한 기내증식
In vitro propagation from axillary bud explants of Fatsia japonica Decne. et Planch.
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초록
액아가 포함된 줄기절편을 0.5 mgl−1 BA와 함께 IAA,NAA, 2,4-D를 농도별 (1, 2, 3 mgl−1)로 조합한 MS기본배지에 치상하여 신초를 유도하였다. 액아로부터 신초의 형성은 0.5 mgl−1 BA와 2 mgl−1 IAA를 혼합처리한 배지에서 57%의 효율로 가장 높게 나타났다. 신초로부터 발근은 MS배지에 IBA를 1 mgl−1를 첨가하였을 때 가장 좋았다. 발생한 root의 길이는 평균 4주 후 약 4㎝로 측정되었다.
Abstract
A propagation for Fatsia japonica using axillary bud explants were established. Cultures were initiated from axillary bud explants on MS medium supplemented with IAA (1, 2, 3 mgl.1), 2,4-D (1, 2, 3 mgl.1) or NAA (1, 2, 3 mgl.1) in combination with BA (0.5 mgl.1). The maximum shoot bud formation was obtained in MS medium supplemented with 0.5 mgl.1 BA and 2 mgl.1 IAA after 4 weeks culture. The microshoot rooted within 4 week in MS medium containing 1.0 mgl.1 IBA.
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