황칠나무(Dendropanax morbifera)의 부정근 유도 및 기내증식조건
Induction and in vitro proliferation of adventitious roots in Dendropanax morbifera
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본 실험은 황칠나무의 기내 부정근 유도 및 증식조건의 확립을 목적으로 수행되었다. 우선 황칠나무(Dendropanax morbifera)의 기 내 발아체로부터 부위(잎, 줄기, 뿌리)를 달리하여 부정근을 유도한 결과 잎은 줄기나 뿌리보다 양호한 부정근의 유도를 보였다. 또한 유도된 부정근을 이용하여 옥신의 종류(IAA, IBA, NAA와 2.4-D) 에 따른 부정근 유도율을 조사한 결과 IBA와 NAA는 IAA와 2.4-D 보다 높은 유도율을 보였다. IBA의 농도에 따른 유도율과 증식효 율은 IBA가 1.0 mg/L첨가되었을 때 가장 높은 유도 및 증식효율을 보였다. 최적의 액체배지조건을 확인하고자 sucrose의 농도와 염농 도를 달리하여 실험한 결과 1/2MS 배지는 MS 배지보다 10%정도 높은 증식을 보였고 sucrose는 30 g/L 첨가 되었을 때 가장 높은 생 중량과 건중량을 나타냈다. 액체배양된 황칠나무의 부정근을 각각 1/2MS 배지에 30 g/L sucrose, 3.0 mg/L IBA가 첨가된 5L 용량의 생물반응기에 4주간 배양한 대조구와 2주후 IBA의 농도를 1.0으로 낮추어 배양한 실험구에서 2주후 IBA의 농도를 낮추어 배양한 실 험구에서 약 2배 높은 부정근의 증식량을 보였다. 본 실험에서는 황칠나무의 종자발아체를 이용하여 부정근의 유도 및 증식조건에 필요한 기내배양조건을 조사하였고 2차적으로 유도된 부정근을 이 용하여 플라스크와 생물반응기 배양을 통한 효율적인 증식조건을 조사하였다.
Abstract
Dendropanax morbifera (Araliaceae) is an endemic species in Korea and distributed in the southern part of Korea. The roots and stems of this plant have been used for folk medicine for the treatment of migraine headache, dysmenorrheal, and remove wind dampness and for Vanishes production. Production of adventitious roots in D. morbifera by in vitro cultures could be used as alternatives materials. Leaf, stem, and root segments from D. morbifera seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the leaf segment. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximum induction of adventitious root was obtained on medium with 1.0 mg/L IBA. In liquid culture, growth of root was best 1/2MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. This study demonstrated for the first time to produce adventitious roots in D. morbifera.
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