기내 배양 사과 대목 M.9의 순화 후 휘묻이 번식 시 발근 관련 생리적 특성 변화
Changes of Root Physiology of Tissue Cultured M.9 Apple Rootstock after Layering
1
조회
0
다운로드
1
인용
0
참고문헌
초록
본 실험은 사과 왜성대목 M.9 조직배양묘를 12회 이상 계대배양 시켜 발근, 순화 후 포장에서 휘묻이에 의해 번식을 할 경우, 복토된 발근 예정 부위의 호르몬, 무기성분 등의 변화와 발근에 미치는 영향을 보고자 실시하였다. 휘묻이 후 첫 부정근 출현 일수는 기내유래 M.9는 25일, 일반 M.9는 30일이었다. 발근율은 기내유래 M.9는 100%였으며 일반 M.9는 83%였다. 휘묻이 할 줄기 부분의 IAA 함량은 기내유래 M.9가 일반 M.9에 비해 높았으나 휘묻이 20일 및 30일 후에는 일반 M.9가 더 높았다. 그러나 ABA 함량은 일반 M.9가 휘묻이 직전, 10일 후 및 20일 후의 기내유래 M.9에 비해 훨씬 더 많았다. 기내 유래 대목의 질소, 붕소, 망간, 아연의 함량은 모두 일반 M.9 보다 유의하게 많 았다. 기내유래 대목의 sucrose, glucose, fructose, maltose 함량과 일반 기외대목의 함량 간에는 유의성이 인정되지 않았다. 기내 유래 및 일반 M.9 대목의 리그닌 변화는 휘묻 이 전, 10일 후, 30일 후에는 고도의 유의성이 인정되었다. 그러므로 기내유래 대목의 발근력의 증가는 당류와 호르몬의 함량 변화 보다는 무기성분과 리그닌 함량 변화에 더 많 이 기인하는 것으로 보인다.
Abstract
This work was conducted to evaluate the efects of rooting on tissue cultured M.9 (Malus domesticaBork. cv, tcM.9) after layering in field. of first root in shoot, rooting ratio, contents of indole-3-acetic acid (IAA), abscisic acid (ABA), inorganic matters, sugars, and lignin in rooting areas of stems by layering. First root in shoot of tcM.9 and natural M.9 appeared 25 and 30 days after layering (DAL), respectively. Rooting ratio was much higher in tcM.9 than in natural M.9. The content of IAA was higher in tcM.9 than in natural M.9 before layering, but it was reversed at 20 and 30 DAL. In contrast, the content of ABA was much higher in natural M.9 than in tcM.9 in case of both before and 10 and 20 DAL. The contents of N, B, Mn, and Zn were significantly higher in tcM.9 than in of sugars in tcM.9 had the similar pattern of the contents of inorganic materials. There were statisticaly significant differences in the contents of sucrose and glucose at 30 DAL as well as the content of maltose at 20 and 30 DAL. The content of lignin was significantly higher in tcM.9 than in natural M.9 before layering and 10 and 30 DAL while there was no difference 20 DAL. Therefore, improvement of rooting ability in the tissue cultured root stock M.9 might be due to the changes of inorganic matters or lignin rather than that of sugars and hormones.
참고문헌 (19)
- Biasi LA, "Use of etiolation in plant propagation", 26, pp.309~314 (1996)
- Chistine AW, "Micropropagation of somecold-hardy dwarfing rootstock for apple", pp.1~6 (1991)
- Domoto PA, "Low temperature tolerance of ‘StarkspurSupreme Delicious’ on nine rootstock in Iowa NC-140cooperative planting", pp.224~229 (1991)
- Eugene J, "Rapid productionmethods for Ottawa-3 rootstock and branched applenursery stock", pp.1416~26 1419 (1991)
- Gomaa AH, "Effects of various promoterson bud burst and side branching of MM Egyptian J of Hort 16", sti(gr), pp.1~8 (1989)
- Goo YB, "A study on mass propagation of Larixleptolepis gordon by cutting and its rooting mechanism" (1995), Seoul Univ
- Howard BH, "Increase during winter in capacity forroot regeneration in detached shoots of fruit tree rootstocks", pp.912~208 13 (1965)
- Karhu ST,, "The effect of differentcarbohydrates on the rooting of micropropagated appleshoots and their adaption after transplantation", 30, pp.87~101 (1995)
- Kim IY, "Changes in calcium contents of leaf and fruitduring growth effect of foliar sprays of calcium on fruitquality of ‘niitaka’ pear" (2004), Kyungpook Univ
- Lee KH, "Growth andflowering responses and changes of gibberellins andabscisic acid contents by cold treatment and cultivationtemperature on chrysanthemum cv", 45, pp.354~358 (2004)
- Pawlicki N, "The effects of benzyladenineand gibberellic acid on adventitious root formation inapple stem clises", agro(me 12), pp.783~788 (1992)
- Zhu ML, "A study of the effect ofPyrus betuli folia Bunge as a nurse stock on the rootingabelity of apple dwarfing rootstocks", pp.20~21 (1994)
- Quamme HA, "Improved rooting of Ottawa3 apple rootstock by softwood cuttings using micropropagatedplants as a cutting source", pp.170~173 (1994)
- Roy CR, "Rootstocks for fruit crops Application of near infrared spectroscopy forquality evaluation of apple fruit", pp.136~138
- Mullins MG, "Induction ofadventitious rooting in vitro in difficult-to-propagate cultivarsof apple", pp.1~4 (1982)
- Stolz LP, "The effects of girdling upon rootinitiation carbohydrates and amino acids", pp.744~751 (1966)
- Webster CA, "Micropropagation of theapple rootstock M effect of sustained subculture onapparent rejuvenation in vitro", pp.421~426 (1989)
- Yae BW, "Factors affecting shootproliferation and root initiation of apple ‘Fuji’ in vitro", pp.353~358 (1986)
- Yoo YK, "Seasonal variation in rooting ability starch and solublesugar contents in cuttings of white forsythia", 37, pp.554~560 (1996)