배 ‘원황’의 재분화와 발근에 미치는 식물생장조절물질과 탄소원의 영향
Effect of plant growth regulators and carbon source on the shoot regeneration and rooting of ‘Wonhwang’ pear (Pyrus pyrifolia L.)
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초록
본 연구의 목적은 배 ‘원황’의 효율적인 재분화 조건을 규명하기 위해 NAA 0.01, 0.05, 0.1, 0.5 mg/L과 BA 3, 5, 10 mg/L 을 조합하여 처리한 결과, NAA 0.05 mg/L, BA 3 mg/L의조성에 plant agar 8 g/L와 sucrose 30 g/L의 MS 배지에서재분화율이 13.3%로 나왔다. 기존의 ‘신고’와 ‘황금배’의발근에 사용하였던 배지 조성과 ‘배연3호’의 발근 배지조성에서는 ‘원황’ 품종의 발근이 전혀 이뤄지지 않았다. 식물생장조절물질이 ‘원황’의 발근에 미치는 영향을 알아보기 위하여 NAA 0.5, 1.0 mg/L, IBA 0.3, 0.5, 1.0 mg/L 을 조합하여 처리한 결과 IBA 1.0 mg/L와 NAA 1.0 mg/L 의 조합에 1/4 LS vitamin이 포함된 배지에서 발근률이24%로 나타났으며 효율적인 ‘원황’의 발근을 위해서는탄소원으로 glucose 7.5 g/L가 사용되었다.
Abstract
The aim of this research is to establish shoot regeneration system for ‘Wonhwang’ pear (Pyrus pyrifolia L.) using various concentrations of 1-Naphthaleneacetic acid (NAA) 0.01, 0.05, 0.1, 0.5 mg/L in combination with benzylaminopurine (BA) 3, 5, 10 mg/L. Medium containing 4.4 g/L of Murashige and Skoog (MS) medium with vitamins containing 8 g/L of plant agar and 30 g/L of sucrose with NAA 0.05 mg/L and BA 3 mg/L showed 13.3% of shoot regeneration rate. ‘Wonhwang’ showed no root growth on existing rooting media of P. pyrifolia cv. Niitaka, ‘Whangkeumbae’ and ‘Bae Yun No. 3’. We evaluated the effect of concentration and kinds of plant growth regulators and carbon source to establish efficient rooting condition for ‘Wonhwang’ pear. In the result of using various concentrations of NAA 0.5 mg/L and 1.0 mg/L in combination with indolebutyric acid (IBA) 3, 5, 10 mg/L, rooting rate of 24% was observed using 1/4 Linsmaier and Skoog (LS) medium supplemented with 7.5 g/L glucose as carbon source and IBA 1.0 mg/L with NAA 1.0 mg/L.
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