대두 calmoduine유전자 SCaM-5를 발현하는 형질전환 토마토의 병 저항성 검정
SCaM-5, a soybean calmodulin, is required for disease resistance against both a bacterial and fungal pathogen in tomato, Lycopersicum esculentum
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Abstract
The calmodulin as a Ca2+-binding protein mediates cellular Ca2+ signals in response to a wide array of stimuli in higher eukaryotes. Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca2+ signals. SCaM-5 is a soybean calmodulin that is involved in plant defense signaling. Here, we constructed a SCaM-5 cDNA under control of CaMV 35S promoter and transformed it into tomato (Lycopersicon esculentum). The constitutive over-expression of SCaM-5 in tomato plants exhibited a high levels of pathogenesis-related (PR) gene expression, and conferred an enhanced resistance to two fungal pathogen (Phytophthora capsici, Fusarium oxysporum), and a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, this results collectively suggest that SCaM-5 plays an important role in plant defense of tomato.