Soybean mosaic virus의 외피단백질유전자 형질전환 담배의 Heterologous potyvirus에 대한 저항성
Resistance to Heterologous Potyvirus in Nicotiana tabacum Expressing the Coat Protein Gene of Soybean mosaic virus
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초록
콩 모자이크 바이러스 계통 중 최근 급속히 확산되고 있으며, 병원성이 비교적 강한 것으로 알려진 G5H와 G7H의 CP 유전자를 분리하여 클로닝하고, 염기서열을 확인하였으며, 확인된 CP 유전자들을 pCAMBIA3301 binary vector에 클로닝하여 형질전환용 운반체를 제작하였고, 이를 Agrobacterium을 이용하여 담배를 형질전환한 후, PPT에 대한 저항성을 보이는 형질전환 담배식물을 대상으로 potyvirus인 PVY를 접종하여 저항성을 검정하였다. 그 결과, 형질전환 담배식물체는 병징의 발현이 지연되고, 발병후에도 회복되는 경향을 보여, CP 유전자의 형질전환에 의해 바이러스 저항성 식물체를 얻을 수 있음을 나타내었다.
Abstract
The coat protein (CP) gene of the G5H and G7H strains of Soybean mosaic virus (SMV) were cloned, sequenced and transformed into the tobacco plant (Nicotiana tabacum. cv. Havana SR1) via Agrobacterium-mediated transformation. Transformation was confirmed by PCR and phosphinothricin (PPT) treatment and the expression of SMV CP gene in transgenic tobacco plant was investigated by ELISA using SMV antibody. The development of disease symptom was delayed and the symptom was alleviated in the transgenic plants inoculated with Potato virus Y (PVY) strain, PVY8 or PVY10. Here we show that the virus resistant transgenic plants could be developed by CP gene transformation.
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