SCaM4 과발현 형질전환 감자의 농업적 특성 및 감자 무름병 저항성 평가
Agronomic characteristics and field resistance to bacterial soft rot of transgenic potato overexpressing the soybean calmodulin 4 gene (SCaM4)
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초록
SCaM4 과발현 감자의 무름병에 대한 저항성은 대비 품종인 ‘대지’에 비해 실내･외 검정에서 저항성인 것으로나타났으며, SCaM4 과발현 SCaM4-4 계통의 경우 실내·외에서 무름병 저항성이 강하였다. SCaM4 과발현 형질전환 감자 계통들에 대한 포장 검정 결과 공시된 4 계통은 꽃색, 줄기형 등에서 대조품종인 대지와 차이가 없었다. 괴경특성 및 수량성의 경우 주당 괴경수는 유사했으나 과발현체의 경우 줄기 무름병 저항성으로 동화산물의생성 및 집적이 원활하여 괴경 무게 증가하여 수량이 증가하는 경향성을 보였다. 실내외검정에서 무름병에 강한저항성을 보인 SCaM4-4 라인은 대조품종인 대지와 실질적인 동등성을 확인할 수 있어 향후 식품학적 안전성과환경위해성 평가가 이루어진다면 실용화 가능성이 클 것으로 사료된다.
Abstract
We performed in vitro assay and field trials to assess levels of changes in intrinsic properties and resistance against soft rot of the potato cv. Dejima upon the introduction of a soybean calmodulin 4 gene (SCaM4). Field trials with four lines overexpressing SCaM4 gene were conducted over two seasons, and harvested tubers were evaluated in bioassay for resistance to Pectobacterium carotovorum ssp. carotovorum. The SCaM4 transgenic potato lines inoculated with 108CFU/ml of P. carotovorum ssp. carotovorum showed enhanced resistance compared to control. Among the SCaM4 transgenic lines, the transgenic line SCaM4-4 exhibited the highest tolerance to soft rot in vitro assays, so did in field trials. In the field trial, the soft rot resistance of SCaM4-4 line was more than 5 times higher compared to that of control cultivar,Dejima. The major agronomic characteristics of the SCaM4transgenic lines were not different from those of the nontransgenic ‘Dejima’. The result demonstrated that the transformation of a calmodulin 4 gene was a successful strategy in development of potato cultivar enhanced to soft rot.
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