대두 칼모듈린 단백질, GmCaM-4를 발현하는 형질전환 감자의 무름병 저항성 확인
Identification of disease resistance to soft rot in transgenic potato plants that overexpress the soybean calmodulin-4 gene (GmCaM-4)
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Calmodulin (CaM) mediates cellular Ca2+ signals in the defense responses of plants. We previously reported that GmCaM-4 and 5 are involved in salicylic acidindependent activation of disease resistance responses in soybean (Glycine max). Here, we generated a GmCaM-4 cDNA construct under the control of the cauliflower mosaic virus (CaMV) 35S promoter and transformed this construct into potato (Solanum tuberosum L.). The constitutive over-expression of GmCaM-4 in potato induced high-level expression of pathogenesis-related (PR) genes, such as PR-2, PR-3, PR-5, phenylalanine ammonia-lyase (PAL), and proteinase inhibitorII (pinII). In addition, the transgenic potato plants exhibited enhanced resistance against a bacterial pathogen, Erwinia carotovora ssp. Carotovora (ECC), that causes soft rot disease and showed spontaneous lesion phenotypes on their leaves. These results strongly suggest that a CaM protein in soybean, GmCaM-4, plays an important role in the response of potato plants to pathogen defense signaling.
Abstract
Calmodulin (CaM) mediates cellular Ca2+ signals in the defense responses of plants. We previously reported that GmCaM-4 and 5 are involved in salicylic acidindependent activation of disease resistance responses in soybean (Glycine max). Here, we generated a GmCaM-4 cDNA construct under the control of the cauliflower mosaic virus (CaMV) 35S promoter and transformed this construct into potato (Solanum tuberosum L.). The constitutive over-expression of GmCaM-4 in potato induced high-level expression of pathogenesis-related (PR) genes, such as PR-2, PR-3, PR-5, phenylalanine ammonia-lyase (PAL), and proteinase inhibitorII (pinII). In addition, the transgenic potato plants exhibited enhanced resistance against a bacterial pathogen, Erwinia carotovora ssp. Carotovora (ECC), that causes soft rot disease and showed spontaneous lesion phenotypes on their leaves. These results strongly suggest that a CaM protein in soybean, GmCaM-4, plays an important role in the response of potato plants to pathogen defense signaling.
참고문헌 (45)
- Bevan M, "Binary Agrobacterium vectors for plant transformation", Nucleic Acids Res, 12, pp.8711~8721 (1984)
- Bouché N, "Plantspecific calmodulin-binding proteins", Annu Rev Plant Biol, 56, pp.435~466 (2005)
- Bush DS, "Calcium regulation in plant cells and its role in signaling", Annu Rev Plant Physiol Plant Mol Biol, 46, pp.95~122 (1995)
- Cheng SH, "Calcium signaling through protein kinases. The Arabidopsis calciumdependent protein kinase gene family", Plant Physiol, 129, pp.469~485 (2002)
- Cho MJ, "Reciprocal regulation of mammalian nitric oxide synthase and calcineurin by plant calmodulin isoforms", Biochemistry, 37, pp.15593~15597 (1998)
- 전현진, "애기장대 칼모듈린 결합 단백질 AtCBP63을 발현시킨 형질전환 감자의 무름병 저항성 증가", Journal of Plant Biotechnology, 38(1), pp.62~68 (2011), 한국식물생명공학회
- Chung WS, "Identification of a calmodulin-regulated soybean Ca2+-ATPase (SCA1) that is located in the plasma membrane", Plant Cell, 12, pp.1393~1407 (2000)
- Day IS, "Analysis of EF-hand-containing proteins in Arabidopsis", Genome Biol, 3 (2002)
- Dietrich RA, "Arabidopsis mutants simulating disease resistance response", Cell, 77, pp.565~577 (1994)
- Dixon RA, "Early events in the activation of plant defense responses", Annu Rev Phytopathol, 32, pp.479~501 (1994)
- Gifford JL, "Comparing the calcium binding abilities of two soybean calmodulins: towards understanding the divergent nature of plant calmodulins", Plant Cell, 25, pp.4512~4524 (2013)
- 이신우, "Expressional Characterization of Dehydroascorbate Reductase cDNA in lransgenic Potato Plants", Journal of Plant Biology(한국식물학회지), 51(1), pp.35~41 (2008), 한국식물학회
- Goodman RN, "The hypersensitive reaction in plants to pathogens: a resistance phenomenon" (1994), APS Press
- MN Heo WD, "Involvement of specific calmodulin isoforms in salicylic acid-independent activation of plant disease resistance responses", Proc Natl Acad Sci USA, 96, pp.766~771 (1999)
- Hoeflich KP, "Calmodulin in action: diversity in target recognition and activation mechanisms", Cell, 108, pp.739~742 (2002)
- Jackson AO, "Plant-microbe interactions: life and death at the interface", Plant Cell, 8, pp.1651~1668 (1996)
- Kim MC, "Calcium and calmodulin-mediated regulation of gene expression in plants", Mol Plant, 2, pp.13~21 (2009)
- Kondo R, "A point mutation in a plant calmodulin is responsible for its inhibition of nitric-oxide synthase", J Biol Chem, 274, pp.36213~36218 (1999)
- Report of Global Atmosphere Watch, "Korea Meterological Adminstration", 177~ (2008)
- Lee SH, "Different regulation of Ca2+", calmodulindependent enzymes by plant calmodulin isoforms and free Ca2+ concentration, 2000, pp.350, 299~306
- Lee SH, "Identification of a novel divergent calmodulin isoform from soybean which has differential ability to activate calmodulin-dependent enzymes", J Biol Chem, 270, pp.21806~21812 (1995)
- Luan S, "Calmodulins and calcineurin B-like proteins:calcium sensors for specific signal response coupling in plants", Plant Cell, 14(Suppl), pp.S389~S400 (2002)
- McAinsh MR, "Encoding specificity in Ca2+ signaling systems", Trends Plant Sci, 3, pp.32~36 (1998)
- McAinsh MR, "Shaping the calcium signature", New Phytol, 181, pp.275~294 (2009)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plantarum, 15, pp.473~497 (1962)
- 박찬영, "Pathogenesis-related Gene Expression by Specific Calmodulin Isoforms Is Dependent on NIM1, a Key Regulator of Systemic Acquired Resistance", Molecules and Cells, 18(2), pp.207~213 (2004), 한국분자세포생물학회
- Park HC, "Pathogenand NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor", Plant Physiol, 135, pp.2150~2161 (2004)
- 박형철, "Functional Analysis of the Stress-Inducible Soybean Calmodulin Isoform-4 (GmCaM-4) Promoter in Transgenic Tobacco Plants", Molecules and Cells, 27(4), pp.475~480 (2009), 한국분자세포생물학회
- Park HC, "Specificity of sequences reconized by the Zinc finger homeodomain protein, GmZF-HD1 in soybean", Phytochemistry, 71, pp.1832~1838 (2010)
- Park HC, "Pathogen-induced binding of the soybean zinc finger homeodomain proteins GmZF-HD1 and GmZF-HD2 to two repeats of ATTA homeodomain binding site in the calmodulin isoform 4 (GmCaM4)promoter", Nucl Acids Res, 35, pp.3612~3623 (2007)
- Poovaiah BW, "Regulated expression of a calmodulin isoform alters growth and development in patato", J Plant Physiol, 149, pp.553~558 (1996)
- Rao SS, "Overexpression of GmCaM4 in soybean enhances resistance to pathogens and tolerance to salt stress", Mol Plant Pathol, 15, pp.145~160 (2014)
- Reddy AS, "Calcium: silver bullet in signaling", Plant Sci, 160, pp.381~404 (2001)
- Ryals JA, "Systemic acquired resistance", Plant Cell, 8, pp.1809~1819 (1996)
- Snedden WA, "Calmodulin, calmodulin-related proteins and plant responses to the environment", Trends Plant Sci, 3, pp.299~304 (1998)
- Snedden WA, "Calmodulin as a versatile calcium signal transducer in plants", New Phytol, 151, pp.35~66 (2001)
- 손황배, "SCaM4 과발현 형질전환 감자의 농업적 특성 및 감자 무름병 저항성 평가", Journal of Plant Biotechnology, 39(4), pp.295~299 (2012), 한국식물생명공학회
- Takezawa D, "Calmodulin gene family in potato: developmental and touch-induced expression of the mRNA encoding a novel isoform", Plant Mol Biol, 27, pp.693~703 (1995)
- The Arabidopsis Genome Initiative, "Analysis of the genome sequence of the flowering plant Arabidopsis thaliana", Nature, 408, pp.796~815 (2002)
- Visser RGF, "Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments", Plant Mol Biol, 12, pp.329~337 (1989)
- Yamakawa H, "Transcriptionally and post-transcriptionally regulated response of 13 calmodulin genes to tobacco mosaic virusinduced cell death and wounding in tobacco plant", Eur J Biochem, 268, pp.3916~3929 (2001)
- Yang T, "Calcium", calmodulin-mediated signal network in plants, 2003, pp.8, 505~512
- Yang T, "Characterization of the calmodulin gene family in wheat:structure, chromosomal location, and evolutionary aspects", Mol Gen Genet, 252, pp.684~694 (1996)
- Yoo JH, "Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis", J Biol Chem, 280, pp.3697~3706 (2005)
- Zhu JK, "Salt and drought stress signal transduction in plants", Annu Rev Plant Biol, 53, pp.247~273 (2002)
