생물반응기를 이용한 Lilium Iongiflorum ‘Geogia’의 대량번식
Micropropagation of Lilium Iongiflorum ‘Geogia’ by Using Bioreactor.
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초록
자구를 형성 및 비대시키기 위하여 BA 0.5 mg/L와 IAA 0.5 mg/L가 첨가된 MS 배지에서 Lilium longiflorum ‘Geogia’의 신초를 증식하였다. 증식된 신초 cluster를 5 L air lift 생물반응기에 배양하였다. 자구비대는 sucrose 60 g/L 및 1～2배 사이의 MS 배지 염류농도에서 효과적이었다. 재료 투입량은 100 g을 투입하는 것이 자구형성 및 자구비대에 적절하였다. 배양방법은 air-lift 방법이 자구의 형성 및 비대를 촉진하였으며, 주입공기량은 200～300 mL․min-1을 주입하는 것이 자구비대에 효과적이었다.
Abstract
Shoot clusters were induced from bulb scales of Lilium longiflorum Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL·min-1 injection of air was suitable in growth of bulblets.
참고문헌 (20)
- Gerrits MM, "Dry-matter partitioning between bulb and leaves in plantlets of Lilium speciosum regenerated in vitro", 41, pp.461~468 (1992)
- Han BH,, "The formation and growth of bulblets from bulblet sections with swollen basal plate in Lilium Oriental Hybrid Casa Blanca'", 40, pp.747~750 (1999)
- Han BH,, "Effect of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulbscales in Lilium Oriental Hybrid Casa Blanca'", 26, pp.103~107 (1999)
- Lian ML, "growth of Lilium oriental hybrid Casablanca' bulblet using bioreactor culture", 97, pp.41~48 (2002)
- Fukui H, "In vitro bulblet regeneration from bulbscale explants of Lilium japonicum Thunb Effect of plant growth regulators and culture environment", pp.289~297 (1994)
- Murashige T, "A revised medium for rapid growth and biossays with tobacco tissue culture", 15, pp.473~497 (1962)
- Nhut DT, "Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo bulblet culture.", 17, pp.913~916 (1998)
- Niimi Y, "Bulblet productivity of explants from scales stems and tepals of Lilium rubellum Barker", pp.391~394 (1984)
- Niimi Y, "Applicatrion of leaf segment culture to in vitrro bulblets production of six Lilium species"
- Niimi Y, "Vegetative propagation of L Comperison of bulblet formation between scaling and scale segment cultured in vitro", o(zawa t), pp.348~349 (1977)
- Novak FJ, "Tissue culture propagation of Lilium hybrids", 14, pp.191~199 (1981)
- Paek KY,, "Application of bioreactors for large scale micropropagation systems of plants. In vitro Cell. Dev", 37, pp.284~292 (2001)
- Priyadarshi S, "A revised scheme for mass propagation of Easter Lily", 30, pp.193~197 (1992)
- Stimart DP, "Tissue culture of bulb-scale sections for asexual propagation of Lilium longiflorum Thunb", 103, pp.182~184 (1978)
- Takayama S, "Differentiation in Lilium bulbscales grown in vitro Effect of various cultural conditions"
- Takayama S, "Differentiation in Lilium bulbscales grown in vitro Effect of activated charcoal physiological age of bulbs and sucrose concentrations on differentiation and scale leaf formation in vitro", pp.121~125 (1980)
- Takayama S, "Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro", pp.67~74 (1982)
- Takayama S, "The mass propagation of Lilium in vitro by stimulation of multiple adventitious bulb-scale formation and by shake culture", 61, pp.224~228 (1983)
- Tanimoto S, "Stimulating effect of spermine on bulblet formation in bulb-scale segments of Lilium longiflorum.", 15, pp.297~300 (1995)
- Wickremesinhe ERM, "A practical mrthod for the production of flowering Easter lilies from callus cultures", 60, pp.143~152 (1994)