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애기장대 H+-pyrophosphatase 발현 형질전환 배추의 건조스트레스에 대한 생리적 반응

Physiological responses to drought stress of transgenic Chinese cabbage expressing Arabidopsis H+-pyrophosphatase

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초록

Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis H+-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and H2O2-3,3’-diaminobenzidine content when compared to wild type plants.

Abstract

Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis H+-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and H2O2-3,3’-diaminobenzidine content when compared to wild type plants.

참고문헌 (23)

  1. Aiguo D, "Drought under global warming: a review", WIREs Clim Change, 2, pp.45~65 (2011)
  2. Baltels D, "Drought and salt tolerance in plants", Crit Rev Plant Sci, 24, pp.23~58 (2005)
  3. Bhargava S, "Drought stress adaptation: metabolic adjustment and regulation of gene expression", Plant Breed, 132, pp.21~32 (2013)
  4. Dietz-Pfeilstetter A, "Stability of transgene expression as a challenge for genetic engineering", Plant Sci, 179, pp.164~167 (2010)
  5. Gaxiola RA, "Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump", Proc Natl Acad Sci USA, 98, pp.11444~11449 (2001)
  6. Genty B, "The relationship between quantum yield of photosynthetic electro transport and quenching of chlorophyll fluorescence", Biochim Biophys Acta, 990, pp.87~92 (1989)
  7. Guan Q, "Genetic transformation and analysis of rice OsAPx2 gene in Medicago sativa", PLoS ONE, 7, pp.1~8 (2012)
  8. Hammond SM, "Post-transcriptional gene siliencing by double-stranded RNA", Nature Genetics, 2, pp.110~119 (2001)
  9. 한증술, "옥수수 calreticulin 과발현 토마토에서 tobamovirus의 상엽 이동 억제", Journal of Plant Biotechnology, 37(4), pp.567~573 (2010), 한국식물생명공학회
  10. Hirschi K, "Vacuolar H+", Ca2+ transport: who’s directing the traffic?, 2001, pp.6, 100~104
  11. Krasensky J, "Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks", J Exp Bot, 63, pp.1593~1608 (2012)
  12. Li J, "Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development", Science, 310, pp.121~125 (2005)
  13. Liu J, "The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance", Proc Natl Acad Sci USA, 97, pp.3730~3734 (2000)
  14. Lu Y, "Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.)", PLoS ONE, 8, pp.1~12 (2013)
  15. 박미희, "애기장대 H+-PPase(AVP1) 과발현 배추에서 바이오매스 증가와 내염성 향상", Journal of Plant Biotechnology, 39(4), pp.253~260 (2012), 한국식물생명공학회
  16. Park S, "Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants", Proc Natl Acad Sci USA, 102, pp.18830~18835 (2005)
  17. Pasapula V, "Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought- and salt tolerance and increases fibre yield in the field conditions", Plant Biotech J, 9, pp.88~99 (2010)
  18. Shou H, "Expression of an active tobacco mitogenactivated protein kinase kinase kinase enhances freezing tolerance in transgenic maize", Proc Natl Acad Sci USA, 101, pp.3298~3303 (2004)
  19. Thapa G, "An insight into the drought stress induced alterations in plants", Biol Plantarum, 55, pp.603~613 (2011)
  20. Wang W, "Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance", Planta, 218, pp.1~14 (2003)
  21. Wu Q, "Ectopic expression of Arabidopsis glutaredoxin AtGRX17 enhances thermotolerance in tomato", Plant Biotechnol J, 10, pp.945~955 (2012)
  22. Yang H, "Enhanced phosphorus nutrition in monocots and dicots over-expressing a phosphorus-responsive type I H+-pyrophosphatase", Plant Biotech J, 5, pp.735~745 (2007)
  23. Zhu JK, "Regulation of ion homeostasis under salt stress", Curr Opin Plant Biol, 6, pp.441~445 (2003)

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