Oligopeptide transporter 관여 유전자 도입 형질전환벼의 고온스트레스 내성 증진
Overexpression of an oligopeptide transporter gene enhances heat tolerance in transgenic rice
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Rice (Oryza sativa) cultivars show an impairment of growth and development in response to abiotic stresses such as drought, salinity, heat and cold at the early seedling stage. The tolerance to heat stress in plants has been genetically modulated by the overexpression of heat shock transcription factor genes or proteins. In addition to a high temperature-tolerance that has also been altered by elevating levels of osmolytes, increasing levels of cell detoxification enzymes and through altering membrane fluidity. To examine the heat tolerance in transgenic rice plants, three OsOPT10 overexpressing lines were characterized through a physiological analysis, which examined factors such as the electrolyte leakage (EL), soluble sugar and proline contents. We further functionally characterized the OsOPT10 gene and found that heat induced the expression of OsOPT10 and P5CS gene related proline biosynthesis. It has been suggested that the expression of OsOPT10 led to elevated heat tolerance in transgenic lines.
Abstract
Rice (Oryza sativa) cultivars show an impairment of growth and development in response to abiotic stresses such as drought, salinity, heat and cold at the early seedling stage. The tolerance to heat stress in plants has been genetically modulated by the overexpression of heat shock transcription factor genes or proteins. In addition to a high temperature-tolerance that has also been altered by elevating levels of osmolytes, increasing levels of cell detoxification enzymes and through altering membrane fluidity. To examine the heat tolerance in transgenic rice plants, three OsOPT10 overexpressing lines were characterized through a physiological analysis, which examined factors such as the electrolyte leakage (EL), soluble sugar and proline contents. We further functionally characterized the OsOPT10 gene and found that heat induced the expression of OsOPT10 and P5CS gene related proline biosynthesis. It has been suggested that the expression of OsOPT10 led to elevated heat tolerance in transgenic lines.
참고문헌 (29)
- Ahuja I, "Plant molecular stress responses face climate change", Trends Plant Sci, 15, pp.664~674 (2010)
- Allen RD, "Use of transgenic plants to study antioxidant defenses", Free Rad Biol Med, 23, pp.473~479 (1997)
- Bray EA, "Plant responses to water deficit", Trends Plant Sci, 2, pp.48~54 (1997)
- Cagnac O, "AtOPT6 Transports Glutathione Derivatives and Is Induced by Primisulfuron", Plant Physiology, 135, pp.1378~1387 (2004)
- Csonka LN, "Prokaryotic osmoregulation:genetics and physiology", Annual Review of microbiology, 45, pp.569~606 (1991)
- Delauney AJ, "Proline biosynthesis and osmoregulation in plants", Plant J, 4, pp.215~223 (1993)[DOI]
- Dionisio-Sese ML, "Antioxidant response of rice seedlings to salinity stress", Plant Sci, 135, pp.1~9 (1998)
- Foucaud C, "Specificity of peptide transport systems in Lactococcus lactis: evidence for a third system which transports hydrophobic di-and tripeptides", Journal of bacteriology, 177(16), pp.4652~4657 (1995)
- Hauser M, "Multiplicity and regulation of genes encoding peptide transporters in Saccharomyces cerevisiae", Molecular membrane biology, 18, pp.105~112 (2001)
- Higgins CF, "ABC transporters: from microorganisms to man", Annual review of cell biology, 8, pp.67~113 (1992)
- Howarth CJ, "Genetic improvements of tolerance to high temperature. Abiotic stresses: plant resistance through breeding and molecular approaches" (2005), Howarth Press Inc
- Igarashi Y, "Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the statement of the gene and salt tolerance in Oryza sativa L", Plant Mol Biol, 33, pp.857~865 (1997)
- 정유진, "Expression analysis and characterization of rice oligopeptide transport gene (OsOPT10) that contributes to salt stress tolerance", Journal of Plant Biotechnology, 37(4), pp.483~493 (2010), 한국식물생명공학회
- Kavi Kishor PB, "Overexpression of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants", Plant Physiology, 108(4), pp.1387~1394 (1995)
- -pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants, "Plant Physiol", 1995, 1387~1394
- Kropff MJ, "Modeling the Impact of Climate Change on Rice production in Asia", pp.27~50 (1995), IRRI and CAB International
- Lichtentaler HK, "Vegetation stress: an introduction to the stress concept in plants", J PlantPhysiol, 148, pp.4~14 (1995)
- Lubkowitz MA, "An oligopeptide transport gene from Candida albicans", Microbiology, 143, pp.387~396 (1997)
- Nagai T, "Differences between rice and wheat in temperature responses of photosynthesis and plant growth", Plant Cell Physiol, 50, pp.744~755 (2009)
- Paulsen IT, "The POT family of transport proteins", Trends Biochem Sci, 10, pp.404~ (1994)
- Pollard A, "Enzyme activities in concentrated solutions of glycinebetaine and other solutes", Planta, 144, pp.291~298 (1979)
- Popp, M., "Environment and Plant Metabolism : Flexibility and Acclimation", pp.199~215 (1995), Bios Scientific oxford
- Roosens NH, "Proline metabolism in the wild-type and in a salt tolerant mutant of Nictiana plumbaginifolia studied by 13C-nuclear magnetic resonance imaging", Plant Physiol, 121, pp.1281~1290 (1999)
- 강시용, "AZCA 저항성 돌연변이 세포주로부터 선발 육성한 내염성 벼 돌연변이 계통의 특성 검정", Journal of Plant Biotechnology, 34(1), pp.61~68 (2007), 한국식물생명공학회
- Stacey MG, "Expression analysis of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction", Planta, 223, pp.291~305 (2006)
- Steiner HY, "The PTR family: a new group of peptide transporters", Molecular microbiology, 16, pp.825~834 (1995)
- Theocharis A, "Physiological and molecular changes in plants grown at low temperatures", Planta, 235, pp.11091~11105 (2012)
- Wahid A, "Heat tolerance in plants: An overview", Environ. Exp. Bot, 61, pp.199~223 (2007)
- Yancey PH, "Water Stress, Osmolytes and Proteins", Amer. Zool, 41, pp.699~709 (2001)