본문 바로가기
KERD
논문KCI 등재

NaCl 처리에 따른 감자 (Solanum tuberosum L.) 기내 식물체의 내염성 및 Proline 함량 변화

Salt tolerance and proline accumulation of potato (Solanum tuberosum L.) in vitro plants to NaCl treatment

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류농학

초록

In order to compare salt tolerance among potato cultivars (Solanum tuberosum L.), in vitro plants of each cultivar were cultured on the Murashige and Skoog’s medium containing different levels of NaCl (0, 75, 150, and 225 mM). The suitable level of NaCl for a comparison of the shoot and root growth between potato cultivars was 75mM. Shoot length and weight were better in ‘Dejima’, ‘Superior’, ‘Jayoung’, and ‘Haryeong’ than in other cultivars such as ‘Goun’ and ‘Atlantic’. Normal root growth was observed in ‘Seohong’, ‘Superior’, and ‘Haryeong’, while ‘Goun’, ‘Atlantic’, ‘Dejima’, ‘Jowon’, and ‘Chuback’ showed no root. Proline contents in all the cultivars increased as affected by NaCl levels, then the increase amount was fewer in the cultivars showed a better growth in both shoot and root than in others showed a poor growth. As a result of this study, ‘Superior’ and ‘Haryeong’ were determined as salt tolerant cultivars and therefore using these cultivars it would be possible to potato production at salt arable land such as reclaimed tidal.

Abstract

In order to compare salt tolerance among potato cultivars (Solanum tuberosum L.), in vitro plants of each cultivar were cultured on the Murashige and Skoog’s medium containing different levels of NaCl (0, 75, 150, and 225 mM). The suitable level of NaCl for a comparison of the shoot and root growth between potato cultivars was 75mM. Shoot length and weight were better in ‘Dejima’, ‘Superior’, ‘Jayoung’, and ‘Haryeong’ than in other cultivars such as ‘Goun’ and ‘Atlantic’. Normal root growth was observed in ‘Seohong’, ‘Superior’, and ‘Haryeong’, while ‘Goun’, ‘Atlantic’, ‘Dejima’, ‘Jowon’, and ‘Chuback’ showed no root. Proline contents in all the cultivars increased as affected by NaCl levels, then the increase amount was fewer in the cultivars showed a better growth in both shoot and root than in others showed a poor growth. As a result of this study, ‘Superior’ and ‘Haryeong’ were determined as salt tolerant cultivars and therefore using these cultivars it would be possible to potato production at salt arable land such as reclaimed tidal.

참고문헌 (48)

  1. Amira MS, "Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.)", J Saudi Soc Agricul Sci, 10, pp.7~15 (2011)
  2. Arvin MJ, "Screening potato cultivars and wild species to abiotic stresses using an electrolyte leakage bioassay", J Agric Sci Technol, 10, pp.33~42 (2008)
  3. Asish KP, "Salt tolerance and salinity effects on plants: a review", Ecotox Environ Safety, 60, pp.324~349 (2005)
  4. Bates LS, "Rapid determination of free proline for water stress studies", Plant Soil, 39, pp.205~207 (1973)
  5. Bilski JJ, "The response of four potato cultivars to chloride and sulfate salinity", Am Potato J, 65, pp.85~90 (1988)
  6. Bruns S, "Shoot development and tuber yield of several potato cultivars under high salt concentrations at different stages of development", Potato Res, 33, pp.23~32 (1990)
  7. Burgutin AB, "In vitro selection of potato for tolerance to sodium chloride", Russ J Plant Physio, 143, pp.524~531 (1996)
  8. Cano EA, "Responses to NaCl sress of cultivated and wild tomato species and their hybrids in callus cultures", Plant Cell Rep, 15, pp.791~794 (1996)
  9. Delauney AJ, "Proline biosynthesis and osmoregulation in plants", Plant J, 4, pp.215~223 (1993)
  10. Feitosa De Lacerta C, "Plant growth and solute accumulation and distribution in two sorghum genotypes under NaCl stress", Braz J Plant Physiol, 13, pp.270~284 (2001)
  11. Fukutaku Y, "Sources of proline nitrogen in water-stressed soybean II. Fate of 5N-labeled protein", Plant Physiol, 61, pp.622~628 (1984)
  12. Gulati A, "In vitro selection of salt-resistant Vigna radiata (L.) Wilczek plants by adventitious shoot formation from cultured cotyledon explants", J Plant Physiol, 142, pp.99~102 (1993)
  13. Hasegawa PM, "Plant cellular and molecular responses to high salinity", Annu Rev Plant Physiol Plant Mol Biol, 51, pp.463~499 (2000)
  14. Hmida-Sayari A, "Overexpression of Delta(1)-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants", Plant Sci, 169, pp.746~752 (2005)
  15. Jaarsma R, "Effect of salt stress on growth, Na+ accumulation and proline metabolism in potato (Solanum tuberosum)", PLoS ONE, 8, pp.e60183~ (2013)
  16. Jefferies RA, "Evaluation of seedling selection for salinity tolerance in potato (Solanum tuberosum L.)", Euphytica, 88, pp.207~213 (1996)
  17. Khavarinejad RA, "Effects of NaCl on photosynthetic pigments, saccharides, and chloroplast ultrastructure in leaves of tomato cultivars", Photosynthetica, 35, pp.151~154 (1998)
  18. Khrais T, "Relative salinity tolerance of potato cultivars assessed by in vitro screening", Amer J of Potato Res, 75, pp.207~210 (1998)
  19. Kim HS, "In vitro selection of salt-resistant Solanum tuberosum L. varieties", J Kor Soc Hort Sci, 36, pp.172~178 (1995)
  20. 김선, "토양염농도에 따른 감자 품종들의 생장특성", 한국작물학회지, 58(2), pp.85~90 (2013), 한국작물학회[DOI]
  21. Levy D, "Influence of water and soil salinity on emergence and early development of potato (Solanum tuberosum L.) cultivars and effect of physiological age of seed tubers", Potato Res, 36, pp.335~340 (1993)
  22. Levy D, "Adaptation of potato to high temperatures and salinity—a review", Potato Res, 84, pp.487~506 (2007)
  23. Maas EV, "Crop tolerance to saline sprinkling water", Plant Soil, 89, pp.273~284 (1985)
  24. Maas EV, "Crop salt tolerance-current assessment", J Irrig Drain Div Proc Am Soc Civil Eng, 103, pp.115~134 (1977)
  25. Mansour MMF, "Protection of plasma membrane of onion epidermal cells by glycine betaine and proline against NaCl stress", Plant Physiol Biochem, 36, pp.767~772 (1998)
  26. Martinez CA, "In vitro salt tolerance and proline accumulation in Andean potato (Solanum spp.) differing in frost resistance", Plant Sci, 116, pp.177~184 (1996)
  27. Morpurgo R, "Correlation between potato clones grown in vivo and in vitro under sodium chloride stress conditions", Plant Breed, 107, pp.80~82 (1991)
  28. Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol plantarum, 15, pp.473~497 (1962)
  29. Naik PS, "Comparison of tissue culture and whole plant responses to salinity in potato", Plant Cell Tissue and Organ Culture, 33, pp.273~280 (1993)
  30. Nanjo T, "Biologica functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana", Plant J, 18, pp.185~193 (1999)
  31. Paleg LG, "Proline and glycine betaine influence protein solvation", Plant Physiol, 75, pp.974~978 (1984)
  32. Paliwal KV, "Effect of saline irrigation water on the yield of potato", Indian J Agricultural Sci, 50, pp.1~33 (1980)
  33. Queiros F, "In vitro selection of salt tolerant cell lines in Solanum tuberosum L", Biologia Plantarum, 51, pp.728~734 (2007)
  34. Rahnama H, "The effect of NaCl on proline accumulation in potato seedlings and calli", Acta Physiol Plantarum, 26, pp.263~270 (2004)
  35. Rains DW, "Cell Culture and Somatic Cell Genetics of Plants", pp.537~547 (1986), Academic Press Inc
  36. Rhoades JD, "Irrigation of Agricultural Crops", pp.1089~1142 (1990), American Society of Agronomy
  37. Ruiz Carrasco KB, "Identification of two phenotypes of Arabidopsis thaliana under in vitro salt stress conditions", Biologia Plantarum, 51, pp.436~442 (2007)
  38. Rural Development Administration, "The potato", pp.208~209 (2011), RDA
  39. Silva JAB, "Microtuberization of Andean potato species (Solanum spp.) as affected by salinity", Scientia Horticulturae, 89, pp.91~101 (2001)
  40. Steward CR, "The rate of proline accumulation in halophytes", Planta, 120, pp.279~289 (1974)
  41. Szabados L, "Proline: a multifunctional amino acid", Trends in Plant Sci, 15, pp.89~97 (2010)
  42. Tai M, "Salinity Tolerance in Plants, Strategies for Crop Improvement", pp.301~320 (1984), Wiley
  43. 탕리, "다양한 환경스트레스에 대한 감자2품종의 감수성 분석", Journal of Plant Biotechnology, 30(4), pp.405~410 (2003), 한국식물생명공학회
  44. van Hoorn JW, "Effect of saline water on soil salinity and on water stress, growth, and yield of wheat and potatoes", Agric Water Mgmt, 23, pp.247~265 (1993)
  45. Werner JE, "Arabidopsis mutants with reduced response to NaCl and osmostic stress", Physiol Plantarum, 93, pp.659~666 (1995)
  46. 윤대진, "식물의 고염 스트레스에 대한 반응 및 적응기작(총설)", Journal of Plant Biotechnology, 32(1), pp.1~14 (2005), 한국식물생명공학회
  47. Zidan M, "Does salinity reduce growth in maize root epidermal cells by inhibiting their capacity for cell wall acidification", Plant Physiol, 93, pp.7~11 (1990)

관련 논문AI추천

이 간행물의 다른 논문 (9건)