벼(Oryza Sativa L.) 내염성 품종과 감수성 품종의 염 처리 시 아미노산 및 단백질 함량 차이
Amino Acid and Protein Contents in the Seedlings of Salt-Tolerant and Salt-Susceptible Rice Cultivars
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NaCl을 처리하였을 때 벼의 잎과 뿌리에서 proline을 포함한 아미노산 함량과 단백질 함량 변화의 차이를 측정하여 벼 내염성 품종(CI5309)과 감수성 품종(IR28)간 특성을 알아보기 위하여 본 실험을 실시하였다. 잎에서 proline 함량은 내염성 품종인 CI5309에 더 많이 축적되었으며 뿌리보다는 잎에 축적되는 proline이 내염성에 관련될 가능성이 더 높았다. NaCl 처리 7일 후에 -aminobutyric acid, -aminoapidic acid, alanine, serine, isoleucine, leucine, phenylalanine, tyrosine 및 valine 등의 아미노산 함량이 내염성 및 감수성 품종에서 증가하는 경향이었으나 그 폭은 감수성 품종에서 훨씬 더 컸다. NaCl을 처리한 벼 잎에서 감수성 품종인 IR28에 비해 내성품종인 CI5309에서 34 KDa와 55 KDa의 단백질 함량이 증가하였다. 55 KDa의 단백질은 내염성 품종과 감수성 품종 모두에서 가장 많은 함량을 보였다.
Abstract
The changes of the amino acid content including proline and protein content in response to NaCl treatment were investigated in between the salt-resistant variety CI5309 and salt-susceptible variety IR28 of rice. The amino acid contents such as -aminoapidic acid, -aminobutyric acid, alanine, serine, isoleucine, leucine, pheylalanine, tyrosine, and valine increased more in the salt-resistant rice CI5309 than in the salt-susceptible rice IR28 at seven days after NaCl treatment. The 34 KDa and 55 KDa protein content were increased more in the CI5309 than in the IR28 in response to NaCl treatment. A 55 KDa protein was major protein in the two rice varieties. The content of proline increased more in the leaves of CI5309 than in those of IR28 after NaCl treatment. It means that the increased proline in the leaves may be more important factor than the proline in the roots for the salt tolerance in rice.
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